FKF1 flavin-binding, kelch repeat, f box 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: FKF1
Official Full Name: flavin-binding, kelch repeat, f box 1
Primary source: TAIR:AT1G68050
Locus tag: AT1G68050
See related: Araport:AT1G68050
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ''flavin-binding; ADO3; f box 1; f box 1''; flavin-binding; kelch repeat; T23K23.10; T23K23_10
Summary: Encodes FKF1, a flavin-binding kelch repeat F box protein, is clock-controlled, regulates transition to flowering. Forms a complex with GI on the CO promoter to regulate CO expression.
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Genomic context

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See FKF1 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 2

Sequence:: Chromosome: 1; NC_003070.9 (25508639..25510907)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Disrupting FKF1 homodimerization increases FT transcript levels in the evening by enhancing CO stabilization.
Title: Disrupting FKF1 homodimerization increases FT transcript levels in the evening by enhancing CO stabilization.
GI plays a pivotal role in CO stability for the precise control of flowering by coordinating balanced functional properties of FKF1 and ZTL.
Title: GIGANTEA Regulates the Timing Stabilization of CONSTANS by Altering the Interaction between FKF1 and ZEITLUPE.
results indicate that COP1 homodimers serve as basic building blocks that assemble into COP1 multimeric complexes with diverse target proteins; light-activated FKF1 inhibits COP1 homodimerization, mainly by destabilizing 440-669kDa COP1 complexes, resulting in the repression of CONSTANS-degrading COP1 activity in the late afternoon in long days, but not in short days, thereby regulating photoperiodic flowering
Title: Light-dependent suppression of COP1 multimeric complex formation is determined by the blue-light receptor FKF1 in Arabidopsis.
FKF1 role in the circadian clock and flowering.
Title: Decoys Untangle Complicated Redundancy and Reveal Targets of Circadian Clock F-Box Proteins.
FKF1 inhibits CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-dependent CO degradation. Light-activated FKF1 has no apparent effect on COP1 stability but can interact with and negatively regulate COP1. We show that FKF1 can inhibit COP1 homo-dimerization. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time.
Title: The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering.
The genetic, physiological, and biochemical evidence, when taken together, leads us to propose that PIF1 and CTG10 coexist, and even accumulate, in the nucleus in darkness, but that, following illumination, CTG10 assists in reducing PIF1 amounts, thus promoting the completion of seed germination and subsequent seedling development.
Title: KELCH F-BOX protein positively influences Arabidopsis seed germination by targeting PHYTOCHROME-INTERACTING FACTOR1.
study demonstrates that FKF1 protein stabilizes CONSTANS (CO)protein in the afternoon in long days; together with CO transcriptional regulation, FKF1 protein controls robust FLOWERING LOCUS T FT) mRNA induction through multiple feedforward mechanisms that accurately control flowering timing
Title: FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering.
Both LKP2 and ZTL suppress early flowering, especially under short-day (noninductive) conditions, and that such suppression is dependent on FKF1.
Title: LOV KELCH PROTEIN2 and ZEITLUPE repress Arabidopsis photoperiodic flowering under non-inductive conditions, dependent on FLAVIN-BINDING KELCH REPEAT F-BOX1.
This result indicates that the conformational change in the loop region represents a major change in the FKF1- light-oxygen-voltage domain photoreaction.
Title: Kinetics of conformational changes of the FKF1-LOV domain upon photoexcitation.
ZTL, FKF1, and LKP2 together regulate TOC1 and PRR5 degradation and are major contributors to determining the period of circadian oscillation and enhancing robustness.
Title: F-box proteins FKF1 and LKP2 act in concert with ZEITLUPE to control Arabidopsis clock progression.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_105475.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within chloroplast relocation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within circadian rhythm	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of cellulose biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of flower development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to blue light	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: flavin-binding, kelch repeat, f box 1

NP_564919.1

''flavin-binding, kelch repeat, f box 1'' (FKF1); FUNCTIONS IN: ubiquitin-protein ligase activity, signal transducer activity; INVOLVED IN: response to blue light, positive regulation of flower development, circadian rhythm, ubiquitin-dependent protein catabolic process, regulation of transcription; LOCATED IN: cellular_component unknown; EXPRESSED IN: whole plant, root tip, male gametophyte, leaf, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: PAC motif (InterPro:IPR001610), Galactose oxidase/kelch, beta-propeller (InterPro:IPR011043), Kelch repeat type 1 (InterPro:IPR006652), PAS fold (InterPro:IPR013767), PAS (InterPro:IPR000014), Kelch repeat type 2 (InterPro:IPR011498), F-box domain, Skp2-like (InterPro:IPR022364), Kelch-type beta propeller (InterPro:IPR015915); BEST Arabidopsis thaliana protein match is: Galactose oxidase/kelch repeat superfamily protein (TAIR:AT5G57360.1); Has 8646 Blast hits to 5789 proteins in 832 species: Archae - 91; Bacteria - 2197; Metazoa - 2116; Fungi - 922; Plants - 2047; Viruses - 0; Other Eukaryotes - 1273 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

25508639..25510907

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_105475.4 → NP_564919.1 flavin-binding, kelch repeat, f box 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_564919.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9C9W9, Q9M648

UniProtKB/TrEMBL

A0A178W8F6, A0A5S9WQF2, A0A654EM32

Conserved Domains (5) summary

PLN02193
Location:292 → 595

PLN02193; nitrile-specifier protein

PRK05784
Location:2 → 69

PRK05784; phosphoribosylamine--glycine ligase; Provisional

PRK13558
Location:53 → 163

PRK13558; bacterio-opsin activator; Provisional

sd00038
Location:305 → 353

Kelch; KELCH repeat [structural motif]

pfam12937
Location:211 → 256

F-box-like