FAB1A 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: FAB1A
Official Full Name: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A
Primary source: TAIR:AT4G33240
Locus tag: AT4G33240
See related: Araport:AT4G33240
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: F4I10.170; F4I10_170; FORMS APLOID AND BINUCLEATE CELLS 1A
Summary: Encodes a protein that is predicted to act as a 1-phosphatidylinositol-3-phosphate (PtdIns3P) 5-kinase based on its homology to Fab1 from yeast. It contains an FYVE domain required for binding to PtdIns3P-containing membranes in yeast, as well as a Cpn60_TCP1 homology domain plus a kinase domain. fab1a/fab1b pollen grains not viable and have defective vacuolar organization. FAB1A and FAB1B complement the enlarged vacuolar phenotype of the fission yeast ste12delta mutant.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See FAB1A in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 13

Sequence:: Chromosome: 4; NC_003075.7 (16029209..16038324, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

overexpression of FAB1A-GFP reassembles SNX2b-mRFP on the endosomal membrane despite the absence of SNX1.
Title: Overexpression of FAB1A-GFP recruits SNX2b on the endosome membrane in snx1-1 mutant in Arabidopsis.
A loss of FAB1 function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane, indicating that FAB1, or its product PtdIns(3,5)P2, mediates the maturation process of the late endosomes.
Title: Phosphatidylinositol 3-Phosphate 5-Kinase, FAB1/PIKfyve Kinase Mediates Endosome Maturation to Establish Endosome-Cortical Microtubule Interaction in Arabidopsis.
Data indicate that single and double mutants of phosphatidylinositol 3-phosphate 5-kinases (PI3P5Ks) FAB1A, FAB1B, FAB1C and FAB1D exhibited slow stomatal closure upon ABA treatment.
Title: Rapid structural changes and acidification of guard cell vacuoles during stomatal closure require phosphatidylinositol 3,5-bisphosphate.
Results suggest that impairment of the function of Arabidopsis FAB1A/B might cause a defect in the membrane recycling capabilities of the auxin transporters and inhibit proper auxin transport into the cells in Arabidopsis.
Title: Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters.
Imbalanced expression of FAB1A/B impairs endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification.
Title: Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis.
Activity of FAB1 proteins is essential for the development of viable pollen. [FAB1A]
Title: Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

General gene information

Go to the top of the page Help

NM_001085015.5

Supported by ESTs:

NM_001203970.1

Supported by ESTs:

NM_001342205.1

Supported by ESTs:

NM_001342206.1

Supported by ESTs:

NM_001342207.1

Supported by ESTs:

NM_001342208.1

Supported by ESTs:

NM_119478.7

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IEA Inferred from Electronic Annotation more info
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within endomembrane system organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylinositol metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pollen development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within vacuole organization	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

Go to the top of the page Help

Preferred Names: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A

NP_001078484.4

FORMS APLOID AND BINUCLEATE CELLS 1A (FAB1A); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup (InterPro:IPR016034), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: phosphatidylinositol-4-phosphate 5-kinase family protein (TAIR:AT3G14270.1); Has 8860 Blast hits to 8121 proteins in 458 species: Archae - 591; Bacteria - 19; Metazoa - 3908; Fungi - 1681; Plants - 1001; Viruses - 3; Other Eukaryotes - 1657 (source: NCBI BLink).

NP_001190899.1

FORMS APLOID AND BINUCLEATE CELLS 1A (FAB1A); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup (InterPro:IPR016034), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: phosphatidylinositol-4-phosphate 5-kinase family protein (TAIR:AT3G14270.1).

NP_001320122.1

FORMS APLOID AND BINUCLEATE CELLS 1A (FAB1A); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: phosphatidylinositol-4-phosphate 5-kinase family protein (TAIR:AT3G14270.1); Has 9196 Blast hits to 8390 proteins in 488 species: Archae - 593; Bacteria - 340; Metazoa - 3784; Fungi - 1828; Plants - 987; Viruses - 3; Other Eukaryotes - 1661 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

16029209..16038324 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001203970.1 → NP_001190899.1 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001190899.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178V4L9, A0A5S9XY67, F4JW01

Conserved Domains (3) summary

cd00139
Location:1412 → 1716

PIPKc; Phosphatidylinositol phosphate kinases (PIPK) catalyze the phosphorylation of phosphatidylinositol phosphate on the fourth or fifth hydroxyl of the inositol ring, to form phosphatidylinositol bisphosphate. CD alignment includes type II ...

cd03334
Location:374 → 646

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cd15725
Location:32 → 99

FYVE_PIKfyve_Fab1; FYVE domain found in metazoan PIKfyve, fungal and plant Fab1, and similar proteins
NM_001342206.1 → NP_001328750.1 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178V4L9, A0A1P8B5L8, A0A5S9XY67
NM_001342207.1 → NP_001328749.1 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178V4L9, A0A1P8B5M2, A0A5S9XY67
NM_001342205.1 → NP_001320122.1 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WUR5, Q9SMY5

UniProtKB/TrEMBL

A0A178V4L9, A0A5S9XY67
NM_001342208.1 → NP_001328751.1 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WUR5, Q9SMY5

UniProtKB/TrEMBL

A0A178V4L9, A0A5S9XY67
NM_119478.7 → NP_195050.6 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_195050.6

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WUR5, Q9SMY5

UniProtKB/TrEMBL

A0A178V4L9, A0A5S9XY67

Conserved Domains (3) summary

cd00139
Location:1416 → 1720

PIPKc; Phosphatidylinositol phosphate kinases (PIPK) catalyze the phosphorylation of phosphatidylinositol phosphate on the fourth or fifth hydroxyl of the inositol ring, to form phosphatidylinositol bisphosphate. CD alignment includes type II ...

cd03334
Location:374 → 646

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cd15725
Location:32 → 99

FYVE_PIKfyve_Fab1; FYVE domain found in metazoan PIKfyve, fungal and plant Fab1, and similar proteins
NM_001085015.5 → NP_001078484.4 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078484.4

Status: REVIEWED

UniProtKB/TrEMBL

A0A178V4L9, A0A5S9XY67, F4JW00

Conserved Domains (3) summary

cd00139
Location:1415 → 1719

PIPKc; Phosphatidylinositol phosphate kinases (PIPK) catalyze the phosphorylation of phosphatidylinositol phosphate on the fourth or fifth hydroxyl of the inositol ring, to form phosphatidylinositol bisphosphate. CD alignment includes type II ...

cd03334
Location:373 → 645

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cd15725
Location:32 → 99

FYVE_PIKfyve_Fab1; FYVE domain found in metazoan PIKfyve, fungal and plant Fab1, and similar proteins