DMS3 defective in meristem silencing 3 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: DMS3
Official Full Name: defective in meristem silencing 3
Primary source: TAIR:AT3G49250
Locus tag: AT3G49250
See related: Araport:AT3G49250
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: DEFECTIVE IN MERISTEM SILENCING 3; IDN1; INVOLVED IN DE NOVO 1
Summary: Similar to hinge-domain region of structural maintenance of chromosomes (SMC)proteins.Putative chromosome architecture protein that can potentialy link nucleic acids in facilitating an RNA1-mediated epigenetic modification involving secondary siRNA and spreading of DNA methylation.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See DMS3 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 7

Sequence:: Chromosome: 3; NC_003074.8 (18255960..18260998, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that all DDR complex components DNA-Directed RNA Polymerases (DRD1), defective in meristem silencing 3 (DMS3) and RNA-DIRECTED DNA METHYLATION 1 (RDM1) co-localize with RNA polymerase V (Pol V).
Title: CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation.
We found that a substantial set of RdDM loci was commonly de-repressed in apc/c and pol v mutants, and that the defects in RdDM activity resulted from up-regulated DMS3 protein levels, which finally caused reduced Pol V recruitment. DMS3 was ubiquitinated by anaphase-promoting complex/cyclosome(APC/C) for degradation in a D box-dependent manner.
Title: Anaphase-promoting complex/cyclosome regulates RdDM activity by degrading DMS3 in Arabidopsis.
Genome-wide chromatin association of NRPE1 is dependent on all members of a putative chromatin-remodeling complex termed DDR, which includes the proteins DRD1, DMS3 and RDM1.
Title: DDR complex facilitates global association of RNA polymerase V to promoters and evolutionarily young transposons.
DMS11 provides the missing ATPase function for DMS3 and that these proteins cooperate in the RNA-directed DNA methylation pathway to promote transcriptional repression.
Title: Involvement of a GHKL ATPase in RNA-directed DNA methylation in Arabidopsis thaliana.
IDN1 (DMS3) and IDN2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway.
Title: IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana.
This finding implicates a putative chromosome architectural protein DMS3 that can potentially link nucleic acids in facilitating an RNAi-mediated epigenetic modification involving secondary siRNAs and spreading of DNA methylation.
Title: A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_114784.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in gene silencing by siRNA-directed DNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulatory ncRNA-mediated gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RNA polymerase V complex	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: defective in meristem silencing 3

NP_566916.1

DEFECTIVE IN MERISTEM SILENCING 3 (DMS3); BEST Arabidopsis thaliana protein match is: gamma-irradiation and mitomycin c induced 1 (TAIR:AT5G24280.1); Has 82 Blast hits to 81 proteins in 17 species: Archae - 6; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 71; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

18255960..18260998 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_114784.3 → NP_566916.1 defective in meristem silencing 3 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_566916.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94A79, Q9M3A7

UniProtKB/TrEMBL

A0A178VBM0, A0A5S9XJH9, A0A7G2EWE8

Conserved Domains (1) summary

COG1196
Location:54 → 351

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]