PEX5 peroxin 5 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PEX5
Official Full Name: peroxin 5
Primary source: TAIR:AT5G56290
Locus tag: AT5G56290
See related: Araport:AT5G56290
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS PEROXIN 5; ATPEX5; EMB2790; EMBRYO DEFECTIVE 2790; MXK23.3; MXK23_3; peroxin 5
Summary: Encodes the peroxisomal targeting signal type 1 receptor that facilitates peroxisomal protein translocation. It recognizes proteins with the PTS1 consensus sequence (tripeptide SKL or a conserved variant) at the extreme C terminus. The protein has several domains, including C-terminal tetratricopeptide repeat motifs which act in binding the C-terminal ''SKL'' targeting signal. The mechanism of transport has been worked out in other organisms: The receptor recognizes and binds cytosolic PTS1-containing proteins. The PEX5-PTS1 complex binds a PEX14/PEX13 receptor complex at the peroxisome membrane and is translocated into the peroxisome matrix in a process dependent on PEX2,PEX10, and PEX12. In the peroxisome matrix, PEX5 releases its cargo and is recycled to the cytosol in a process dependent on PEX1, PEX4, PEX6 and PEX22. It is also involved, in conjunction with PEX7, in PTS1- and PTS2-dependent peroxisomal protein import. RNAi experiments suggest that PEX5 is necessary for the maintenance of both glyoxysomal and leaf peroxisomal functions.
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Genomic context

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See PEX5 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 15

Sequence:: Chromosome: 5; NC_003076.8 (22786496..22792306)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

beyond PEX5 recycling, PEX1 and PEX6 have additional functions in peroxisome homeostasis and perhaps in oil body utilization
Title: A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling.
Growth at elevated temperature did not reduce the fraction of membrane-associated PEX5 in pex4-1 mutants, suggesting that elevated temperature did not restore PEX4 enzymatic function in the mutant.
Title: Elevated growth temperature decreases levels of the PEX5 peroxisome-targeting signal receptor and ameliorates defects of Arabidopsis mutants with an impaired PEX4 ubiquitin-conjugating enzyme.
PEX2 and PEX10 cooperate to support import of matrix proteins into plant peroxisomes and peroxin5 retrotranslocation in Arabidopsis.
Title: Peroxisomal ubiquitin-protein ligases peroxin2 and peroxin10 have distinct but synergistic roles in matrix protein import and peroxin5 retrotranslocation in Arabidopsis.
PEX5 binding to the PEX14N-terminal domain (PEX14N) has no effect on the affinity of PEX5 for a PTS1 containing peptides.
Title: PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor.
PEX7 facilitates PEX5 accumulation and import of PTS1 cargo into peroxisomes
Title: Interdependence of the peroxisome-targeting receptors in Arabidopsis thaliana: PEX7 facilitates PEX5 accumulation and import of PTS1 cargo into peroxisomes.
Pex5p recognizes Cat1 in a manner distinct from typical PTS1.
Title: Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import.
The Arabidopsis pex5-1 mutant is specifically defective in PTS2 (but not PTS1) protein import into peroxisomes, probably because of reduced interaction with the PEX7 PTS2 receptor.
Title: The Arabidopsis peroxisomal targeting signal type 2 receptor PEX7 is necessary for peroxisome function and dependent on PEX5.
A genetic screen for peroxisome defective mutants revealed a pex5 mutant that is sucrose dependent during germination and resistant to the inhibitory effects of IBA on root elongation.
Title: Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes.
Pex5p and Pex7p play different roles in the maintenance of glyoxysomal and leaf peroxisomal functions in plants
Title: Differential contribution of two peroxisomal protein receptors to the maintenance of peroxisomal functions in Arabidopsis.
The expression profile of AtLACS6, AtLACS7, AtPEX5, and AtPEX7 revealed that AtLACS6 and 7 have distinct patterns of expression.
Title: AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_125012.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within protein targeting to peroxisome	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to auxin	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info
located_in plastid	HDA more info	PubMed

General protein information

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Preferred Names: peroxin 5

NP_200440.1

peroxin 5 (PEX5); CONTAINS InterPro DOMAIN/s: Tetratricopeptide TPR-1 (InterPro:IPR001440), Tetratricopeptide-like helical (InterPro:IPR011990), Tetratricopeptide repeat-containing (InterPro:IPR013026), Tetratricopeptide repeat (InterPro:IPR019734); BEST Arabidopsis thaliana protein match is: Tetratricopeptide repeat (TPR)-like superfamily protein (TAIR:AT3G11540.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

22786496..22792306

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_125012.5 → NP_200440.1 peroxin 5 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_200440.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O82467, Q9FMA3

UniProtKB/TrEMBL

A0A178UC50, A0A5S9YER1

Conserved Domains (3) summary

COG0457
Location:472 → 726

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:590 → 618

TPR; TPR repeat [structural motif]

pfam00515
Location:658 → 690

TPR_1; Tetratricopeptide repeat