U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    CAO chloroplast signal recognition particle component (CAO) [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 819358, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    CAO
    Official Full Name
    chloroplast signal recognition particle component (CAO)
    Primary source
    TAIR:AT2G47450
    Locus tag
    AT2G47450
    See related
    Araport:AT2G47450
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    CHAOS; CHLOROPLAST SIGNAL RECOGNITION PARTICLE 43; CPSRP43; T30B22.25
    Summary
    A component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. It is downregulated in response to high light. It recognizes the DPLG motif in Lhcb1.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See CAO in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    1
    Sequence:
    Chromosome: 2; NC_003071.7 (19472573..19474387)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Signal transduction histidine kinase Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene myb domain protein 12 Neighboring gene thioredoxin family protein Neighboring gene DUF3511 domain protein, putative (DUF3511)

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone. Siegel A, et al. J Mol Biol, 2020 Dec 4. PMID 33188783, Free PMC Article
    2. A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Klimyuk VI, et al. Plant Cell, 1999 Jan. PMID 9878634, Free PMC Article
    3. Involvement of the chloroplast signal recognition particle cpSRP43 in acclimation to conditions promoting photooxidative stress in Arabidopsis. Klenell M, et al. Plant Cell Physiol, 2005 Jan. PMID 15659446
    4. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43. Stengel KF, et al. Science, 2008 Jul 11. PMID 18621669
    5. Mechanism of an ATP-independent protein disaggregase: I. structure of a membrane protein aggregate reveals a mechanism of recognition by its chaperone. Nguyen TX, et al. J Biol Chem, 2013 May 10. PMID 23525109, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock.
      Title: Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock.
    2. A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone.
      Title: A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone.
    3. Site-directed mutagenesis identified a class of cpSRP43 mutants that bind tightly to the L18 sequence but are defective in chaperoning full-length chlorophyll a/b-binding proteins. These mutations mapped to hydrophobic surfaces near the bridging helix and the beta-hairpins lining the ankyrin repeat motifs of cpSRP43, suggesting that these regions are potential sites for interaction with the client transmembrane domains .
      Title: Two distinct sites of client protein interaction with the chaperone cpSRP43.
    4. CpSRP54 drives cpSRP43 to an active state, binding substrate in the H2O compartment. Bidentate interactions with Alb3 drive it to partial inactivity, selectively releasing LHCP transmembrane domains in a productive unloading complex at the membrane.
      Title: Conformational dynamics of a membrane protein chaperone enables spatially regulated substrate capture and release.
    5. cpSRP43 chromodomain 3 interacts with a linear motif in the Alb3 C-terminal tail. Study describes the structural basis for selectivity of CPSRP43 chromodomains 2 and 3 for their respective ligands cpSRP54 and Alb3.
      Title: Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction.
    6. results support the conclusion that substrate binding to the chloroplast SRP is modulated by protein structural dynamics in which a major role of cpSRP54 is to improve substrate binding efficiency to the cpSRP.
      Title: Regulation of Structural Dynamics within a Signal Recognition Particle Promotes Binding of Protein Targeting Substrates.
    7. The action of cpSRP43 can be dissected into two steps, initial recognition, during which cpSRP43 binds specifically to a recognition motif on the surface of the aggregate, followed by aggregate remodeling.
      Title: Mechanism of an ATP-independent protein disaggregase: II. distinct molecular interactions drive multiple steps during aggregate disassembly.
    8. A mechanism for recognition of the LHC aggregate by cpSRP43 elucidated by a biophysical and biochemical approach.
      Title: Mechanism of an ATP-independent protein disaggregase: I. structure of a membrane protein aggregate reveals a mechanism of recognition by its chaperone.
    9. cpSRP43 inserts into a dimeric Alb3 translocation pore during cpSRP-dependent delivery of light-harvesting chlorophyll a,b-binding proteins. [SRP43]
      Title: Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein.
    10. The authors report the crystal structure of the chloroplast signal recognition particle (cpSRP) core from Arabidopsis thaliana, with the cpSRP54 tail comprising an arginine-rich motif bound to the second chromodomain of cpSRP43.
      Title: Chromodomains read the arginine code of post-translational targeting.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_130313.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within protein import into chloroplast thylakoid membrane TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to high light intensity IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast envelope HDA PubMed 
    located_in chloroplast thylakoid membrane HDA PubMed 
    located_in cytosol HDA PubMed 
    located_in intracellular anatomical structure TAS
    Traceable Author Statement
    more info
    		 PubMed 
    part_of signal recognition particle, chloroplast targeting IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    chloroplast signal recognition particle component (CAO)
    NP_566101.1
    • CHAOS (CAO); FUNCTIONS IN: chromatin binding; INVOLVED IN: response to high light intensity, protein import into chloroplast thylakoid membrane; LOCATED IN: chloroplast thylakoid membrane, chloroplast, signal recognition particle, chloroplast targeting, chloroplast envelope; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ankyrin repeat-containing domain (InterPro:IPR020683), Chromo domain-like (InterPro:IPR016197), Chromo domain (InterPro:IPR000953), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: ankyrin repeat-containing protein 2 (TAIR:AT4G35450.5); Has 25373 Blast hits to 13489 proteins in 848 species: Archae - 87; Bacteria - 2575; Metazoa - 11620; Fungi - 2006; Plants - 1049; Viruses - 137; Other Eukaryotes - 7899 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      19472573..19474387
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_130313.3 → NP_566101.1  chloroplast signal recognition particle component (CAO) [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_566101.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O22265, Q93V50, Q940I7, Q9SAU3
      UniProtKB/TrEMBL
      A0A5S9X8K6
      Conserved Domains (5) summary
      smart00298
      Location:85 → 133
      CHROMO; Chromatin organization modifier domain
      cd00204
      Location:126 → 236
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      pfam00385
      Location:321 → 368
      Chromo; Chromo (CHRromatin Organization MOdifier) domain
      pfam12796
      Location:135 → 224
      Ank_2; Ankyrin repeats (3 copies)
      sd00045
      Location:129 → 157
      ANK; ANK repeat [structural motif]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O22265.2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools