AMI1 amidase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Official Symbol: AMI1
Official Full Name: amidase 1
Primary source: TAIR:AT1G08980
Locus tag: AT1G08980
See related: Araport:AT1G08980
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: amidase 1; AMIDASE-LIKE PROTEIN 1; ARABIDOPSIS THALIANA TRANSLOCON AT THE OUTER MEMBRANE OF CHLOROPLASTS 64-I; ATAMI1; ATTOC64-I; TOC64-I; TRANSLOCON AT THE OUTER MEMBRANE OF CHLOROPLASTS 64-I
Summary: Encodes an enzyme with similarity to bacterial acylamidohydrolases and exhibits indole-3-acetamide amidohydrolase activity in vitro. This enzyme may be involved in the in vivo biosynthesis of indole-acetic acid from indole-3-acetamide, a native metabolite of A. thaliana. It appears to exist as a monomer.
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Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Two homologous INDOLE-3-ACETAMIDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis.
Title: Two homologous INDOLE-3-ACETAMIDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis.
analysis of amidase 1 in rat and Arabidopsis
Title: Arabidopsis amidase 1, a member of the amidase signature family.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

NM_100769.4

Function	Evidence Code	Pubs
enables amidase activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	IDA Inferred from Direct Assay more info	PubMed
enables indoleacetamide hydrolase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in auxin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within indoleacetic acid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in extracellular region	HDA more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed

NP_563831.1

amidase 1 (AMI1); FUNCTIONS IN: amidase activity, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, indoleacetamide hydrolase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Amidase, conserved site (InterPro:IPR020556), Amidase (InterPro:IPR000120); BEST Arabidopsis thaliana protein match is: translocon at the outer membrane of chloroplasts 64-V (TAIR:AT5G09420.1); Has 18462 Blast hits to 18443 proteins in 2488 species: Archae - 262; Bacteria - 10888; Metazoa - 543; Fungi - 1390; Plants - 376; Viruses - 0; Other Eukaryotes - 5003 (source: NCBI BLink).