E2f3 E2F transcription factor 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: E2f3provided by MGI
Official Full Name: E2F transcription factor 3provided by MGI
Primary source: MGI:MGI:1096340
See related: Ensembl:ENSMUSG00000016477 AllianceGenome:MGI:1096340
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: E2F3b; E2f3a
Summary: Enables DNA binding activity and DNA-binding transcription factor activity. Involved in negative regulation of fat cell proliferation. Acts upstream of or within positive regulation of DNA-templated transcription and positive regulation of cell population proliferation. Predicted to be located in nucleus. Predicted to be part of RNA polymerase II transcription regulator complex. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma and prostate cancer. Orthologous to human E2F3 (E2F transcription factor 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 9.4), limb E14.5 (RPKM 7.5) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 A3.2; 13 13.09 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (30090558..30171172, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (29906575..29986064, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identifying the E2F3-MEX3A-KLF4 signaling axis that sustains cancer cells in undifferentiated and proliferative state.
Title: Identifying the E2F3-MEX3A-KLF4 signaling axis that sustains cancer cells in undifferentiated and proliferative state.
Matrix stiffness modulates hepatic stellate cell activation into tumor-promoting myofibroblasts via E2F3-dependent signaling and regulates malignant progression.
Title: Matrix stiffness modulates hepatic stellate cell activation into tumor-promoting myofibroblasts via E2F3-dependent signaling and regulates malignant progression.
Circ_PGPEP1 Serves as a Sponge of miR-1297 to Promote Gastric Cancer Progression via Regulating E2F3.
Title: Circ_PGPEP1 Serves as a Sponge of miR-1297 to Promote Gastric Cancer Progression via Regulating E2F3.
RB-mediated repression of E2F3 is critical for the maintenance of cell cycle exit and terminal differentiation in adult mouse Sertoli cells.
Title: Retinoblastoma protein represses E2F3 to maintain Sertoli cell quiescence in mouse testis.
Findings suggest that E2F3 has a pivotal role in muscle and bone development, and affect normal mouse growth.
Title: Critical Roles of E2F3 in Growth and Musculo-skeletal Phenotype in Mice.
E2F3a is a novel, upstream regulator of the addiction-mediating transcription factor DeltaFosB in NAc.
Title: Fosb Induction in Nucleus Accumbens by Cocaine Is Regulated by E2F3a.
The study showed that E2f3a, but not E2f3b, overexpression or knockdown in mouse nucleus accumbens regulates cocaine-induced locomotor and place conditioning behavior.
Title: Transcription Factor E2F3a in Nucleus Accumbens Affects Cocaine Action via Transcription and Alternative Splicing.
HIV-1 Tat protein promotes neuronal dysregulation by inhibiting E2F transcription factor 3
Title: HIV-1 Tat protein promotes neuronal dysregulation by inhibiting E2F transcription factor 3 (E2F3).
E2F3 transcription factor variant impairs STAT5 signaling and mammary gland remodeling during pregnancy in mice
Title: An Rb family-independent E2F3 transcription factor variant impairs STAT5 signaling and mammary gland remodeling during pregnancy in mice.
Findings identify E2f3 as a key transcription factor in tumor-associated macrophages (TAMs), which influences the tumor microenvironment and tumor cell metastasis.
Title: E2f3 in tumor macrophages promotes lung metastasis.

Submit: New GeneRIF Correction See all GeneRIFs (57)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (15) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC85656P1 (e-PCR)
- UniSTS:273486

PMC85656P2 (e-PCR)
- UniSTS:273487

PMC85656P3 (e-PCR)
- UniSTS:273488

PMC85656P4 (e-PCR)
- UniSTS:273489

E2f3 (e-PCR), detects polymorphism
- UniSTS:506869

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
contributes_to DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in G1/S transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of fat cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of cell population proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of vascular associated smooth muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in protein import into nucleus	IEA Inferred from Electronic Annotation more info
involved_in protein import into nucleus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase II transcription regulator complex	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: transcription factor E2F3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001289920.1 → NP_001276849.1 transcription factor E2F3 isoform E2f3b

See identical proteins and their annotated locations for NP_001276849.1

Status: VALIDATED

Description

Transcript Variant: This variant (2, also known as E2f3b) differs in the 5' UTR and the 5' coding region and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform E2f3b, which is shorter and has a distinct N-terminus, compared to isoform E2f3a.

Source sequence(s)

AK129048, AK133828, AL513025, BQ176318, BU705601

Consensus CDS

CCDS88434.1

UniProtKB/TrEMBL

Q3TMJ9, Q8R1S8

Related

ENSMUSP00000152368.2, ENSMUST00000222730.2

Conserved Domains (2) summary

cd14660
Location:123 → 223

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:49 → 112

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
NM_001359994.1 → NP_001346923.1 transcription factor E2F3 isoform 3

Status: VALIDATED

Source sequence(s)

AL513025

Consensus CDS

CCDS88435.1

UniProtKB/TrEMBL

A0A1Y7VML1, Q8R1S8

Related

ENSMUSP00000152782.2, ENSMUST00000221536.2

Conserved Domains (2) summary

cd14660
Location:129 → 229

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:55 → 118

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
NM_010093.3 → NP_034223.1 transcription factor E2F3 isoform E2f3a

See identical proteins and their annotated locations for NP_034223.1

Status: VALIDATED

Description

Transcript Variant: This variant (1, also known as E2f3a) represents the longer transcript and encodes the longer isoform (E2f3a).

Source sequence(s)

AK133828, AV509156, BC059262

Consensus CDS

CCDS26413.1

UniProtKB/Swiss-Prot

O35261, Q5T0I6

UniProtKB/TrEMBL

Q3UZJ0

Related

ENSMUSP00000100012.4, ENSMUST00000102948.11

Conserved Domains (2) summary

cd14660
Location:246 → 346

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:172 → 235

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

30090558..30171172 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030247136.2 → XP_030102996.1 transcription factor E2F3 isoform X3

Conserved Domains (2) summary

cd14660
Location:240 → 340

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:165 → 229

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_011244276.4 → XP_011242578.1 transcription factor E2F3 isoform X2

Conserved Domains (2) summary

cd14660
Location:250 → 350

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:175 → 239

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_011244275.4 → XP_011242577.1 transcription factor E2F3 isoform X1

Conserved Domains (2) summary

cd14660
Location:256 → 356

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:181 → 245

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_036157843.1 → XP_036013736.1 transcription factor E2F3 isoform X4

UniProtKB/TrEMBL

Q8R1S8

Conserved Domains (2) summary

cd14660
Location:144 → 244

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:70 → 133

E2F_TDP; E2F/DP family winged-helix DNA-binding domain