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    USP35 ubiquitin specific peptidase 35 [ Homo sapiens (human) ]

    Gene ID: 57558, updated on 10-Dec-2024

    Summary

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    Official Symbol
    USP35provided by HGNC
    Official Full Name
    ubiquitin specific peptidase 35provided by HGNC
    Primary source
    HGNC:HGNC:20061
    See related
    Ensembl:ENSG00000118369 MIM:620959; AllianceGenome:HGNC:20061
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene is upregulated by the let-7a microRNA and reduced expression has been observed in human tumor tissues. [provided by RefSeq, Jul 2017]
    Expression
    Ubiquitous expression in adrenal (RPKM 3.3), brain (RPKM 2.1) and 25 other tissues See more
    Orthologs
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    Genomic context

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    See USP35 in Genome Data Viewer
    Location:
    11q14.1
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (78188919..78237219)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (78122182..78166945)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (77899965..77926278)

    Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene ALG8 alpha-1,3-glucosyltransferase Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5314 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5315 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5316 Neighboring gene H3K27ac hESC enhancer GRCh37_chr11:77851405-77852115 Neighboring gene RNA, U6 small nuclear 126, pseudogene Neighboring gene KCTD21 antisense RNA 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr11:77884885-77885386 Neighboring gene H3K27ac hESC enhancer GRCh37_chr11:77890493-77890992 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3808 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3809 Neighboring gene potassium channel tetramerization domain containing 21 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr11:77921405-77922031 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3810 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5317 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5318 Neighboring gene GRB2 associated binding protein 2 Neighboring gene Sharpr-MPRA regulatory region 2371 Neighboring gene uncharacterized LOC105369402 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5319 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 5320 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:78076162-78077074 Neighboring gene H3K27ac hESC enhancer GRCh37_chr11:78081224-78082174 Neighboring gene zinc finger protein 75C, pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation. Lv T, et al. Int J Oncol, 2023 Oct. PMID 37594129, Free PMC Article
    2. The deubiquitylating enzyme USP35 restricts regulated cell death to promote survival of renal clear cell carcinoma. Wang S, et al. Cell Death Differ, 2023 Jul. PMID 37173391, Free PMC Article
    3. Expansion of DUB functionality generated by alternative isoforms - USP35, a case study. Leznicki P, et al. J Cell Sci, 2018 May 16. PMID 29685892, Free PMC Article
    4. Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin. Tang Z, et al. Clin Transl Med, 2021 Apr. PMID 33931967, Free PMC Article
    5. Ubiquitin-specific Protease 35 Promotes Gastric Cancer Metastasis by Increasing the Stability of Snail1. Ma C, et al. Int J Biol Sci, 2024. PMID 38250150, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ubiquitin-specific Protease 35 Promotes Gastric Cancer Metastasis by Increasing the Stability of Snail1.
      Title: Ubiquitin-specific Protease 35 Promotes Gastric Cancer Metastasis by Increasing the Stability of Snail1.
    2. USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination-mediated degradation.
      Title: USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation.
    3. The deubiquitylating enzyme USP35 restricts regulated cell death to promote survival of renal clear cell carcinoma.
      Title: The deubiquitylating enzyme USP35 restricts regulated cell death to promote survival of renal clear cell carcinoma.
    4. USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.
      Title: USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.
    5. Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin.
      Title: Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin.
    6. Deubiquitinase USP35 restrains STING-mediated interferon signaling in ovarian cancer.
      Title: Deubiquitinase USP35 restrains STING-mediated interferon signaling in ovarian cancer.
    7. Regulation of survivin protein stability by USP35 is evolutionarily conserved.
      Title: Regulation of survivin protein stability by USP35 is evolutionarily conserved.
    8. USP35, regulated by estrogen and AKT, promotes breast tumorigenesis by stabilizing and enhancing transcriptional activity of estrogen receptor alpha.
      Title: USP35, regulated by estrogen and AKT, promotes breast tumorigenesis by stabilizing and enhancing transcriptional activity of estrogen receptor α.
    9. Genome-wide suppressor screen identifies USP35/USP38 as therapeutic candidates for ciliopathies.
      Title: Genome-wide suppressor screen identifies USP35/USP38 as therapeutic candidates for ciliopathies.
    10. Two different USP35 isoforms that localise to different intracellular compartments and have distinct functions.
      Title: Expansion of DUB functionality generated by alternative isoforms - USP35, a case study.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables cysteine-type deubiquitinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cysteine-type deubiquitinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein deubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of protein stability IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin carboxyl-terminal hydrolase 35
    Names
    deubiquitinating enzyme 35
    ubiquitin specific protease 35
    ubiquitin thioesterase 35
    ubiquitin thiolesterase 35
    ubiquitin-specific-processing protease 35
    NP_065849.1
    XP_011543489.1
    XP_011543490.1
    XP_047283288.1
    XP_047283290.1
    XP_047283291.1
    XP_054225458.1
    XP_054225459.1
    XP_054225460.1
    XP_054225461.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_020798.4 → NP_065849.1  ubiquitin carboxyl-terminal hydrolase 35

      See identical proteins and their annotated locations for NP_065849.1

      Status: REVIEWED

      Source sequence(s)
      AB037793, AI263341, AJ586137, AK056231, AP002985, CN287538, DB095282
      Consensus CDS
      CCDS41693.1
      UniProtKB/Swiss-Prot
      Q9P2H5
      Related
      ENSP00000431876.1, ENST00000529308.6
      Conserved Domains (2) summary
      cl02553
      Location:442 → 924
      Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
      pfam00443
      Location:441 → 923
      UCH; Ubiquitin carboxyl-terminal hydrolase

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

      Range
      78188919..78237219
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047427335.1 → XP_047283291.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X1

      UniProtKB/Swiss-Prot
      Q9P2H5

    2. XM_047427334.1 → XP_047283290.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X1

      UniProtKB/Swiss-Prot
      Q9P2H5

    3. XM_047427332.1 → XP_047283288.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X1

      UniProtKB/Swiss-Prot
      Q9P2H5

    4. XM_011545188.3 → XP_011543490.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X2

      See identical proteins and their annotated locations for XP_011543490.1

      UniProtKB/Swiss-Prot
      Q9P2H5
      Related
      ENSP00000434942.1, ENST00000526425.1
      Conserved Domains (2) summary
      cl02553
      Location:173 → 655
      Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
      pfam00443
      Location:172 → 654
      UCH; Ubiquitin carboxyl-terminal hydrolase

    5. XM_011545187.3 → XP_011543489.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X2

      See identical proteins and their annotated locations for XP_011543489.1

      UniProtKB/Swiss-Prot
      Q9P2H5
      Conserved Domains (2) summary
      cl02553
      Location:173 → 655
      Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
      pfam00443
      Location:172 → 654
      UCH; Ubiquitin carboxyl-terminal hydrolase

    RNA

    1. XR_001747930.2 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060935.1 Alternate T2T-CHM13v2.0

      Range
      78122182..78166945
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054369484.1 → XP_054225459.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X1

    2. XM_054369483.1 → XP_054225458.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X1

    3. XM_054369486.1 → XP_054225461.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X2

    4. XM_054369485.1 → XP_054225460.1  ubiquitin carboxyl-terminal hydrolase 35 isoform X2

    RNA

    1. XR_008488421.1 RNA Sequence

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9P2H5.3 GenPept UniProtKB/Swiss-Prot:Q9P2H5

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