Megf8 multiple EGF-like-domains 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Megf8provided by RGD
Official Full Name: multiple EGF-like-domains 8provided by RGD
Primary source: RGD:621190
See related: EnsemblRapid:ENSRNOG00000052687 AllianceGenome:RGD:621190
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Egfl4
Summary: Predicted to enable calcium ion binding activity. Predicted to be involved in several processes, including axon development; embryonic morphogenesis; and left/right pattern formation. Predicted to act upstream of or within several processes, including blood vessel development; embryonic brain development; and embryonic digit morphogenesis. Predicted to be located in nucleus. Predicted to be part of ubiquitin ligase complex. Predicted to be active in basement membrane. Human ortholog(s) of this gene implicated in Carpenter syndrome. Orthologous to human MEGF8 (multiple EGF like domains 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 207.8), Kidney (RPKM 99.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 42

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (90030057..90079423)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (80902236..80951614)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (82184671..82234045)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role and mechanism of miR-871-3p/Megf8 in regulating formaldehyde-induced cardiomyocyte inflammation and congenital heart disease.
Title: Role and mechanism of miR-871-3p/Megf8 in regulating formaldehyde-induced cardiomyocyte inflammation and congenital heart disease.

Submit: New GeneRIF Correction

General gene information

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Markers

RH135154 (e-PCR)
- UniSTS:218431

RH141844 (e-PCR)
- UniSTS:223836

RH129690 (e-PCR)
- UniSTS:212986

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in BMP signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in BMP signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in animal organ morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in aorta development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within aorta development	ISO Inferred from Sequence Orthology more info
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration involved in gastrulation	IEA Inferred from Electronic Annotation more info
involved_in cell migration involved in gastrulation	ISO Inferred from Sequence Orthology more info
involved_in coronary vasculature development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within coronary vasculature development	ISO Inferred from Sequence Orthology more info
involved_in craniofacial suture morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in craniofacial suture morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in determination of digestive tract left/right asymmetry	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within determination of digestive tract left/right asymmetry	ISO Inferred from Sequence Orthology more info
involved_in determination of digestive tract left/right asymmetry	ISS Inferred from Sequence or Structural Similarity more info
involved_in determination of heart left/right asymmetry	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within determination of heart left/right asymmetry	ISO Inferred from Sequence Orthology more info
involved_in determination of heart left/right asymmetry	ISO Inferred from Sequence Orthology more info
involved_in determination of heart left/right asymmetry	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within determination of left/right symmetry	ISO Inferred from Sequence Orthology more info
involved_in embryonic brain development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryonic brain development	ISO Inferred from Sequence Orthology more info
involved_in embryonic digit morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryonic digit morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in embryonic heart tube left/right pattern formation	IEA Inferred from Electronic Annotation more info
involved_in embryonic heart tube left/right pattern formation	ISO Inferred from Sequence Orthology more info
involved_in embryonic heart tube left/right pattern formation	ISS Inferred from Sequence or Structural Similarity more info
involved_in embryonic heart tube morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in embryonic heart tube morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in embryonic heart tube morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in embryonic limb morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in embryonic limb morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in embryonic skeletal system morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in embryonic skeletal system morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in embryonic skeletal system morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in epiboly involved in gastrulation with mouth forming second	IEA Inferred from Electronic Annotation more info
involved_in epiboly involved in gastrulation with mouth forming second	ISO Inferred from Sequence Orthology more info
NOT acts_upstream_of_or_within epithelial cilium movement involved in determination of left/right asymmetry	ISO Inferred from Sequence Orthology more info
involved_in fasciculation of sensory neuron axon	IEA Inferred from Electronic Annotation more info
involved_in fasciculation of sensory neuron axon	ISO Inferred from Sequence Orthology more info
involved_in fasciculation of sensory neuron axon	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within heart development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within left/right pattern formation	ISO Inferred from Sequence Orthology more info
involved_in left/right pattern formation	ISO Inferred from Sequence Orthology more info
involved_in left/right pattern formation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within limb development	ISO Inferred from Sequence Orthology more info
involved_in limb morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smoothened signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of smoothened signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smoothened signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in pharyngeal arch artery morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pharyngeal arch artery morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension involved in axon guidance	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of axon extension involved in axon guidance	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension involved in axon guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein-containing complex assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in regulation of gene expression	ISS Inferred from Sequence or Structural Similarity more info
involved_in smoothened signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within smoothened signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in tissue development	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in basement membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
NOT located_in membrane	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: multiple epidermal growth factor-like domains protein 8

Names: EGF-like domain-containing protein 4; epidermal growth factor-like protein 4; multiple EGF-like domain protein 4; multiple EGF-like domains protein 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053628.2 → NP_446080.1 multiple epidermal growth factor-like domains protein 8 precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

Q9QYP0

UniProtKB/TrEMBL

R9PXW3

Related

ENSRNOP00000074834.1, ENSRNOT00000077313.3

Conserved Domains (10) summary

PLN02153
Location:203 → 484

PLN02153; epithiospecifier protein

cd00041
Location:49 → 139

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00055
Location:1210 → 1259

EGF_Lam; Laminin-type epidermal growth factor-like domain; laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in ...

sd00038
Location:1673 → 1721

Kelch; KELCH repeat [structural motif]

pfam01437
Location:950 → 998

PSI; Plexin repeat

pfam12947
Location:1078 → 1112

EGF_3; EGF domain

pfam13415
Location:1630 → 1680

Kelch_3; Galactose oxidase, central domain

pfam13418
Location:227 → 276

Kelch_4; Galactose oxidase, central domain

pfam13964
Location:1728 → 1775

Kelch_6; Kelch motif

cl00057
Location:1011 → 1035

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

90030057..90079423

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039106569.2 → XP_038962497.1 multiple epidermal growth factor-like domains protein 8 isoform X3

UniProtKB/TrEMBL

A0A8I5ZPN6

Conserved Domains (9) summary

PLN02153
Location:1715 → 1802

PLN02153; epithiospecifier protein

cd00041
Location:49 → 139

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00055
Location:1210 → 1259

EGF_Lam; Laminin-type epidermal growth factor-like domain; laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in ...

sd00038
Location:1673 → 1721

Kelch; KELCH repeat [structural motif]

pfam01437
Location:950 → 998

PSI; Plexin repeat

pfam12947
Location:1078 → 1112

EGF_3; EGF domain

pfam13415
Location:1633 → 1680

Kelch_3; Galactose oxidase, central domain

pfam13418
Location:227 → 276

Kelch_4; Galactose oxidase, central domain

cl21504
Location:2124 → 2163

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...
XM_039106490.2 → XP_038962418.1 multiple epidermal growth factor-like domains protein 8 isoform X2

UniProtKB/TrEMBL

A0A8I5ZPN6

Conserved Domains (9) summary

PLN02153
Location:1715 → 1802

PLN02153; epithiospecifier protein

cd00041
Location:49 → 139

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00055
Location:1210 → 1259

EGF_Lam; Laminin-type epidermal growth factor-like domain; laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in ...

sd00038
Location:1673 → 1721

Kelch; KELCH repeat [structural motif]

pfam01437
Location:950 → 998

PSI; Plexin repeat

pfam12947
Location:1078 → 1112

EGF_3; EGF domain

pfam13415
Location:1633 → 1680

Kelch_3; Galactose oxidase, central domain

pfam13418
Location:227 → 276

Kelch_4; Galactose oxidase, central domain

cl21504
Location:2124 → 2163

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...
XM_006228364.5 → XP_006228426.1 multiple epidermal growth factor-like domains protein 8 isoform X1

UniProtKB/Swiss-Prot

Q9QYP0

Conserved Domains (10) summary

PLN02153
Location:203 → 484

PLN02153; epithiospecifier protein

cd00041
Location:49 → 139

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00055
Location:1207 → 1256

EGF_Lam; Laminin-type epidermal growth factor-like domain; laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in ...

sd00038
Location:1670 → 1718

Kelch; KELCH repeat [structural motif]

pfam01437
Location:947 → 995

PSI; Plexin repeat

pfam12947
Location:1075 → 1109

EGF_3; EGF domain

pfam13415
Location:1627 → 1677

Kelch_3; Galactose oxidase, central domain

pfam13418
Location:227 → 276

Kelch_4; Galactose oxidase, central domain

pfam13964
Location:1725 → 1772

Kelch_6; Kelch motif

cl00057
Location:1008 → 1032

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_039106583.1 → XP_038962511.1 multiple epidermal growth factor-like domains protein 8 isoform X4

Conserved Domains (7) summary

PLN02153
Location:987 → 1074

PLN02153; epithiospecifier protein

cd00055
Location:482 → 531

EGF_Lam; Laminin-type epidermal growth factor-like domain; laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in ...

sd00038
Location:945 → 993

Kelch; KELCH repeat [structural motif]

pfam01437
Location:222 → 270

PSI; Plexin repeat

pfam12947
Location:350 → 384

EGF_3; EGF domain

pfam13415
Location:905 → 952

Kelch_3; Galactose oxidase, central domain

pfam13418
Location:837 → 880

Kelch_4; Galactose oxidase, central domain