Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rasgrf1provided by MGI
Official Full Name: RAS protein-specific guanine nucleotide-releasing factor 1provided by MGI
Primary source: MGI:MGI:99694
See related: Ensembl:ENSMUSG00000032356 AllianceGenome:MGI:99694
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gnrp; Grf1; p190; CDC25; P190-A; CDC25Mm; Grfbeta; Ras-GRF1; p190RhoGEF
Summary: Enables glutamate receptor binding activity. Involved in activation of GTPase activity; neuron projection development; and regulation of small GTPase mediated signal transduction. Acts upstream of or within regulation of NMDA receptor activity; regulation of neuronal synaptic plasticity; and type B pancreatic cell proliferation. Located in cytosol and growth cone. Is expressed in several structures, including alimentary system; genitourinary system; musculoskeletal system; nervous system; and sensory organ. Orthologous to human RASGRF1 (Ras protein specific guanine nucleotide releasing factor 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in cerebellum adult (RPKM 26.4), frontal lobe adult (RPKM 25.1) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E3.1; 9 47.31 cM

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (89791565..89909032)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (89909508..90026979)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of MLL Induces Epigenetic Dysregulation of Rasgrf1 to Attenuate Kras-Driven Lung Tumorigenesis.
Title: Loss of MLL Induces Epigenetic Dysregulation of Rasgrf1 to Attenuate Kras-Driven Lung Tumorigenesis.
Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Macrophages Negatively Affects M1 Polarization and Inflammatory Responses.
Title: Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Macrophages Negatively Affects M1 Polarization and Inflammatory Responses.
RasGRF1 has no effect on self administration for cocaine.
Title: The Inhibition of RasGRF2, But Not RasGRF1, Alters Cocaine Reward in Mice.
p190RhoGEF may play a role in the stage of plasma cell differentiation during T-cell-dependent humoral immune responses.
Title: Over-expression of p190RhoGEF enhances B-cell activation and germinal center formation in T-cell-dependent humoral immune responses.
The deletion of RasGRF1 attenuated myocardial fibrosis and improved cardiac function in diabetic mice through inhibiting inflammation and oxidative stress.
Title: Deletion of RasGRF1 Attenuated Interstitial Fibrosis in Streptozotocin-Induced Diabetic Cardiomyopathy in Mice through Affecting Inflammation and Oxidative Stress.
hypermethylation of RASgrf1 after kainate induced status epilepticus could be reversed with corresponding changes of RASgrf1 expression
Title: RASgrf1, a Potential Methylatic Mediator of Anti-epileptogenesis?
These findings demonstrate that RASgrf1 is closely associated with epilepsy via the aberrant methylation of RASgrf1, and regulating the methylation status of relevant genes might be an intriguing topic in future research on epilepsy.
Title: Association of RASgrf1 methylation with epileptic seizures.
Novel mercaptoacetamide-based class II histone deacetylase inhibitor HDACIW2 treatment regulated the levels of RasGRF1 and p-ERK, and that W2 requires RasGRF1 and ERK signaling to alter dendritic spine formation, suggesting that W2 regulates dendritic spine formation via a RasGRF1/ERK-dependent signaling pathway
Title: A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway.
Data (including data from studies in knockout mice) suggest mechanisms involving RasGRF1 exist to regulate hypothalamic-pituitary-adrenal axis in early-adolescent females; such mechanisms appear absent in younger/older female or adolescent male mice.
Title: RasGRF1 regulates the hypothalamic-pituitary-adrenal axis specifically in early-adolescent female mice.
VLDLR requires RasGRF1/CaMKII to alter dendritic spine formation.
Title: Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.

Submit: New GeneRIF Correction See all GeneRIFs (50)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (2) 1 citation
Endonuclease-mediated (2)
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI844718 (e-PCR)
- UniSTS:165652

D9Mit10 (e-PCR), detects polymorphism
- UniSTS:116776

X59868 (e-PCR)
- UniSTS:125814

D9Mit157 (e-PCR), detects polymorphism
- UniSTS:131710

D9Mit157.1 (e-PCR), detects polymorphism
- UniSTS:131711

D8Rat203 (e-PCR), detects polymorphism
- UniSTS:228270

RH132198 (e-PCR)
- UniSTS:215481

D9Mit240 (e-PCR), detects polymorphism
- UniSTS:131805

STS-L26584 (e-PCR)
- UniSTS:14301

Rasgrf1 (e-PCR), detects polymorphism
- UniSTS:507886

D9Dcr24 (e-PCR), detects polymorphism
- UniSTS:498940

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables receptor tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in activation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in neuron projection development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Ras protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of fibroblast proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of NMDA receptor activity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of Ras protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of neuronal synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic plasticity	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic plasticity	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to endoplasmic reticulum stress	IEA Inferred from Electronic Annotation more info
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within type B pancreatic cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical dendrite	ISO Inferred from Sequence Orthology more info
located_in apicolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ras-specific guanine nucleotide-releasing factor 1

Names: Ras guanine release factor beta; ras-specific nucleotide exchange factor CDC25

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001039655.1 → NP_001034744.1 ras-specific guanine nucleotide-releasing factor 1 isoform 2

See identical proteins and their annotated locations for NP_001034744.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks multiple 3' coding exons and uses an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 2, also known as GRF beta) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC102545, AK140227

Consensus CDS

CCDS40723.1

UniProtKB/TrEMBL

A0A087WS68, Q9QZR7

Related

ENSMUSP00000034909.5, ENSMUST00000034909.11

Conserved Domains (2) summary

pfam00169
Location:24 → 82

PH; PH domain

cl17171
Location:19 → 92

PH-like; Pleckstrin homology-like domain
NM_001357743.1 → NP_001344672.1 ras-specific guanine nucleotide-releasing factor 1 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is 2 aa shorter compared to isoform 1.

Source sequence(s)

AC102545

Conserved Domains (6) summary

smart00147
Location:1021 → 1257

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00325
Location:246 → 427

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

pfam00618
Location:636 → 685

RasGEF_N; RasGEF N-terminal motif

cl02520
Location:925 → 994

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...

cl17171
Location:19 → 155

PH-like; Pleckstrin homology-like domain

cl25508
Location:105 → 218

Spc7; Spc7 kinetochore protein
NM_001357744.1 → NP_001344673.1 ras-specific guanine nucleotide-releasing factor 1 isoform 4

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks multiple 3' coding exons and uses an alternate 3' terminal exon compared to variant 1. The resulting isoform (4) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC102545, DV043084

UniProtKB/TrEMBL

P70317

Conserved Domains (1) summary

cl17171
Location:19 → 108

PH-like; Pleckstrin homology-like domain
NM_011245.2 → NP_035375.1 ras-specific guanine nucleotide-releasing factor 1 isoform 1

See identical proteins and their annotated locations for NP_035375.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC102545, AK079638, AK140380, CD775453

Consensus CDS

CCDS40722.1

UniProtKB/Swiss-Prot

P27671

UniProtKB/TrEMBL

B2RS27

Related

ENSMUSP00000034912.5, ENSMUST00000034912.6

Conserved Domains (6) summary

smart00147
Location:1023 → 1259

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00325
Location:248 → 429

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

pfam00169
Location:24 → 130

PH; PH domain

pfam00618
Location:638 → 687

RasGEF_N; RasGEF N-terminal motif

cl02520
Location:927 → 996

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...

cl17171
Location:19 → 155

PH-like; Pleckstrin homology-like domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

89791565..89909032

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154723.1 → XP_036010616.1 ras-specific guanine nucleotide-releasing factor 1 isoform X1

Conserved Domains (4) summary

smart00147
Location:597 → 833

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

pfam00618
Location:212 → 261

RasGEF_N; RasGEF N-terminal motif

cl02520
Location:501 → 570

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...

cl17171
Location:9 → 160

PH-like; Pleckstrin homology-like domain