Mgat5 mannoside acetylglucosaminyltransferase 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mgat5provided by MGI
Official Full Name: mannoside acetylglucosaminyltransferase 5provided by MGI
Primary source: MGI:MGI:894701
See related: Ensembl:ENSMUSG00000036155 AllianceGenome:MGI:894701
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GlcNAc-TV; 4930471A21Rik; 5330407H02Rik
Summary: Enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within protein N-linked glycosylation. Located in Golgi apparatus. Is expressed in several structures, including alimentary system; brain; integumental system; sensory organ; and urinary system. Orthologous to human MGAT5 (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in colon adult (RPKM 9.9), frontal lobe adult (RPKM 9.4) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 E3; 1 55.71 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (127132450..127413760)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (127204919..127488334)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N-glycosylation by Mgat5 imposes a targetable constraint on immune-mediated tumor clearance.
Title: N-glycosylation by Mgat5 imposes a targetable constraint on immune-mediated tumor clearance.
Loss of N-acetylglucosaminyl transferase V is involved in the impaired osteogenic differentiation of bone marrow mesenchymal stem cells.
Title: Loss of N-acetylglucosaminyl transferase V is involved in the impaired osteogenic differentiation of bone marrow mesenchymal stem cells.
Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.
Title: Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.
Deficiency in Branched Glycosylation in MGAT5 Null or Heterozygous Mice Is Associated with Early-Onset Disease and Increased Severity of Colitis>
Title: Metabolic control of T cell immune response through glycans in inflammatory bowel disease.
Study indicates that Mgat5 genotype affects both behaviour and physical outcomes in response to early life stress, suggesting some shared pathways for both in a mouse model. These results provide a starting point for studying the mechanisms by which protein N-glycosylation mediates the effects of early life adversity.
Title: Mgat5 modulates the effect of early life stress on adult behavior and physical health in mice.
Overexpression of GnT-V exacerbated murine experimental colitis by inducing macrophage dysfunction, thereby enhancing colorectal tumorigenesis
Title: N-Acetylglucosaminyltransferase V exacerbates murine colitis with macrophage dysfunction and enhances colitic tumorigenesis.
Expression of GnT-V was also elevated in bleomycin (BLM)-injected sclerotic skin, and MGAT5(-/-) mice were resistant to BLM-induced skin sclerosis with reduced collagen type 1 alpha1 content.
Title: Oligosaccharide modification by N-acetylglucosaminyltransferase-V in macrophages are involved in pathogenesis of bleomycin-induced scleroderma.
GnT-V regulates the canonical Wnt/beta-catenin signaling pathway by modulating N-glycosylation of Wnt receptors, which changes cancer stem cells in colorectal tumors, causing altered colon tumorigenesis and adenoma progression in Apc(min/+) mice
Title: Post-translational glycoprotein modifications regulate colon cancer stem cells and colon adenoma progression in Apc(min/+) mice through altered Wnt receptor signaling.
this study suggests that the GnT-V expression is inversely correlated with radiation sensitivity in prostate cancer(PCa)cells
Title: Inhibition of N-acetylglucosaminyltransferase V enhances sensitivity of radiotherapy in human prostate cancer.
The GnT-V prevented steatohepatitis progression through modulating lymphocyte and HSC functions.
Title: N-Acetylglucosaminyltransferase V regulates TGF-β response in hepatic stellate cells and the progression of steatohepatitis.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (1) 1 citation

General gene information

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Markers

AI480971 (e-PCR)
- UniSTS:164126

Mgat5 (e-PCR), detects polymorphism
- UniSTS:546863

MT1318 (e-PCR)
- UniSTS:128559

D1Mit218.2 (e-PCR), detects polymorphism
- UniSTS:128560

RH126208 (e-PCR)
- UniSTS:162916

D1Jcs10 (e-PCR), detects polymorphism
- UniSTS:142232

NoName (e-PCR)
- UniSTS:234202

AU049455 (e-PCR)
- UniSTS:145902

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity	ISO Inferred from Sequence Orthology more info
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables glycosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables manganese ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein phosphatase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables protein phosphatase inhibitor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of receptor signaling pathway via STAT	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of receptor signaling pathway via STAT	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein N-linked glycosylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein N-linked glycosylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in perikaryon	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Names: GNT-V; N-acetylglucosaminyl-transferase V; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; beta1,6N-acetylglucosaminyltransferase V; glcNAc-T V

NP_660110.2

EC 2.4.1.155

XP_006529118.1

EC 2.4.1.155

XP_006529119.1

EC 2.4.1.155

XP_006529120.1

EC 2.4.1.155

XP_030110888.1

EC 2.4.1.155

XP_036022125.1

EC 2.4.1.155

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145128.3 → NP_660110.2 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

See identical proteins and their annotated locations for NP_660110.2

Status: VALIDATED

Source sequence(s)

AK030402, BC125533, BY752687

Consensus CDS

CCDS15245.1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Related

ENSMUSP00000038359.5, ENSMUST00000038361.11

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

127132450..127413760

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006529056.5 → XP_006529119.1 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

See identical proteins and their annotated locations for XP_006529119.1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18
XM_006529055.5 → XP_006529118.1 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

See identical proteins and their annotated locations for XP_006529118.1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18
XM_030255028.2 → XP_030110888.1 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18
XM_006529057.5 → XP_006529120.1 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

See identical proteins and their annotated locations for XP_006529120.1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18
XM_036166232.1 → XP_036022125.1 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

UniProtKB/Swiss-Prot

Q8R4G6

UniProtKB/TrEMBL

Q059T5

Related

ENSMUSP00000129166.2, ENSMUST00000171405.2

Conserved Domains (2) summary

pfam15027
Location:7 → 137

DUF4525; Domain of unknown function (DUF4525)

pfam15024
Location:171 → 725

Glyco_transf_18; Glycosyltransferase family 18