Skp1 S-phase kinase-associated protein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Skp1provided by MGI
Official Full Name: S-phase kinase-associated protein 1provided by MGI
Primary source: MGI:MGI:103575
See related: Ensembl:ENSMUSG00000036309 AllianceGenome:MGI:103575
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: OCP2; p19A; 15kDa; EMC19; Skp1a; OCP-II; Tceb1l; p19Skp1; 2610043E24Rik; 2610206H23Rik
Summary: Predicted to enable several functions, including F-box domain binding activity; beta-catenin binding activity; and cullin family protein binding activity. Predicted to contribute to ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process. Acts upstream of or within protein K48-linked ubiquitination and protein monoubiquitination. Located in centrosome and cytosol. Part of Cul7-RING ubiquitin ligase complex and SCF ubiquitin ligase complex. Is expressed in several structures, including central nervous system; early conceptus; genitourinary system; notochord; and sensory organ. Orthologous to human SKP1 (S-phase kinase associated protein 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E18 (RPKM 68.9), CNS E11.5 (RPKM 64.4) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B1.3; 11 31.86 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (52121349..52137685)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (52230522..52246858)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

miR-582-5p targets Skp1 and regulates NF-kappaB signaling-mediated inflammation.
Title: miR-582-5p targets Skp1 and regulates NF-κB signaling-mediated inflammation.
Loss of SKP1 prior to meiosis leads to aberrant localization of DSB repair proteins and a failure in synapsis initiation in meiosis of both males and females as well as a defect in sister chromatid cohesion. FBXO47 interacts with SKP1 and HORMAD1 and targets HORMAD1 for polyubiquitination and degradation in HEK293T cells.
Title: SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.
SKP1 localizes to synapsed chromosome axes and evicts HORMAD proteins from these regions in meiotic spermatocytes. SKP1 maintains synapsis in meiosis of both sexes. SKP1-deficient spermatocytes display premature desynapsis, precocious pachytene exit, loss of PLK1 and BUB1 at centromeres. SKP1-deficient spermatocytes show sharply reduced MPF activity and fail to enter MI despite treatment with okadaic acid.
Title: SKP1 drives the prophase I to metaphase I transition during male meiosis.
Consistent with Skp1a functioning through regulation of Nmnat2, Skp1a knockdown fails to protect axons from Nmnat2 knockdown.
Title: An Atypical SCF-like Ubiquitin Ligase Complex Promotes Wallerian Degeneration through Regulation of Axonal Nmnat2.
Lysine 29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.
Title: Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.
Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex.
Title: Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex.
In this review, deficiency of SKP1A in SN4741 cells closely recapitulates cardinal features of the dopamine (DA) neuron pathology of human Parkinson's disease, such as decreased expression of DA phenotypic markers and cell cycle aberrations.
Title: Genetic reduction of the E3 ubiquitin ligase element, SKP1A and environmental manipulation to emulate cardinal features of Parkinson's disease.
phosphorylated NIPA is degraded in late mitosis in an APC/C(Cdh1)-dependent manner
Title: APC/C(Cdh1)-mediated degradation of the F-box protein NIPA is regulated by its association with Skp1.
Skp1 is a potential modifier in sporadic Parkinson disease neurodegeneration
Title: A sporadic Parkinson disease model via silencing of the ubiquitin-proteasome/E3 ligase component SKP1A.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11Pjn4 (e-PCR), detects polymorphism
- UniSTS:498690

RH137096 (e-PCR)
- UniSTS:219184

Tceb1l (e-PCR), detects polymorphism
- UniSTS:144552

Tceb1l-rs1 (e-PCR), detects polymorphism
- UniSTS:144553

Skp1a (e-PCR), detects polymorphism
- UniSTS:527537

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables F-box domain binding	IEA Inferred from Electronic Annotation more info
enables F-box domain binding	ISO Inferred from Sequence Orthology more info
enables beta-catenin binding	IEA Inferred from Electronic Annotation more info
enables beta-catenin binding	ISO Inferred from Sequence Orthology more info
enables cullin family protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables cullin family protein binding	IEA Inferred from Electronic Annotation more info
enables cullin family protein binding	ISO Inferred from Sequence Orthology more info
enables molecular function activator activity	IEA Inferred from Electronic Annotation more info
enables molecular function activator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin ligase complex scaffold activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin ligase complex scaffold activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-like ligase-substrate adaptor activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like ligase-substrate adaptor activity	ISO Inferred from Sequence Orthology more info
contributes_to ubiquitin-protein transferase activity	IC Inferred by Curator more info	PubMed

Process	Evidence Code	Pubs
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in maintenance of protein location in nucleus	IEA Inferred from Electronic Annotation more info
involved_in maintenance of protein location in nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein monoubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
part_of Cul7-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cul7-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of PcG protein complex	IEA Inferred from Electronic Annotation more info
part_of PcG protein complex	ISO Inferred from Sequence Orthology more info
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SCF ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of SCF ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in gap junction	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: S-phase kinase-associated protein 1

Names: S-phase kinase-associated protein 1A; cyclin-A/CDK2-associated protein p19

NP_035673.3

EC 3.1.1.3

XP_006532849.1

EC 3.1.1.3

XP_036012398.1

EC 3.1.1.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011543.4 → NP_035673.3 S-phase kinase-associated protein 1

See identical proteins and their annotated locations for NP_035673.3

Status: VALIDATED

Source sequence(s)

AK012498, AK078330, BU841654

Consensus CDS

CCDS24669.1

UniProtKB/Swiss-Prot

Q8C5H6, Q9WTX5

UniProtKB/TrEMBL

Q3TL58, Q5SUR3

Related

ENSMUSP00000038744.6, ENSMUST00000037324.12

Conserved Domains (2) summary

cd18322
Location:3 → 127

BTB_POZ_SKP1; BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in S-phase kinase-associated protein 1 (SKP1) and similar proteins

pfam01466
Location:113 → 160

Skp1; Skp1 family, dimerization domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

52121349..52137685

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532786.2 → XP_006532849.1 S-phase kinase-associated protein 1 isoform X1

See identical proteins and their annotated locations for XP_006532849.1

UniProtKB/Swiss-Prot

Q8C5H6, Q9WTX5

UniProtKB/TrEMBL

Q3TL58, Q5SUR3

Conserved Domains (2) summary

cd18322
Location:3 → 127

BTB_POZ_SKP1; BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in S-phase kinase-associated protein 1 (SKP1) and similar proteins

pfam01466
Location:113 → 160

Skp1; Skp1 family, dimerization domain
XM_036156505.1 → XP_036012398.1 S-phase kinase-associated protein 1 isoform X2

Conserved Domains (2) summary

cd18322
Location:2 → 92

BTB_POZ_SKP1; BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in S-phase kinase-associated protein 1 (SKP1) and similar proteins

pfam01466
Location:78 → 125

Skp1; Skp1 family, dimerization domain