Trim63 tripartite motif containing 63 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Trim63provided by RGD
Official Full Name: tripartite motif containing 63provided by RGD
Primary source: RGD:619964
See related: EnsemblRapid:ENSRNOG00000016543 AllianceGenome:RGD:619964
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Murf; Murf1; Rnf28
Summary: Enables ubiquitin-protein transferase activity. Involved in several processes, including regulation of muscle adaptation; response to interleukin-1; and skeletal muscle atrophy. Acts upstream of or within muscle contraction. Located in contractile muscle fiber. Used to study muscular dystrophy. Biomarker of muscular atrophy and muscular dystrophy. Orthologous to human TRIM63 (tripartite motif containing 63). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Muscle (RPKM 585.1), Heart (RPKM 371.4) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 5q36

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (151817209..151831026)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (146533492..146547332)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (152533362..152547138)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MuRF-1 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G-Actin Ubiquitination.
Title: MuRF-1 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G-Actin Ubiquitination.
[Effects of different types of exercise on contractile properties and expressions of MuRF1, PGC-1alpha /FNDC5 in soleus muscle of unloaded rats].
Title: [Effects of different types of exercise on contractile properties and expressions of MuRF1, PGC-1α /FNDC5 in soleus muscle of unloaded rats].
Targeting MuRF1 by small molecules in a HFpEF rat model improves myocardial diastolic function and skeletal muscle contractility.
Title: Targeting MuRF1 by small molecules in a HFpEF rat model improves myocardial diastolic function and skeletal muscle contractility.
Leucine-Rich Diet Improved Muscle Function in Cachectic Walker 256 Tumour-Bearing Wistar Rats.
Title: Leucine-Rich Diet Improved Muscle Function in Cachectic Walker 256 Tumour-Bearing Wistar Rats.
Systematic Review: Models of Changes in Gene Expression of MTOR, MURF-1, and MAFBX in Rats and Mice.
Title: Systematic Review: Models of Changes in Gene Expression of MTOR, MURF-1, and MAFBX in Rats and Mice.
Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading.
Title: Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading.
Exercise down-regulated the mRNA levels of murf1 and atrogin-1, decreased reactive oxygen species level, increased antioxidant capacity, regulated the expression of insulin-like growth factor 1 (IGF1), mechano growth factor (MGF), Neuregulin1 (NRG1) and Myostatin (MSTN), and activated Akt and Erk1/2 signalings in soleus muscle.
Title: Effects of different types of exercise on skeletal muscle atrophy, antioxidant capacity and growth factors expression following myocardial infarction.
UBE2D2 is not the cognate ubiquitinating enzyme for MuRF1
Title: UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension.
Data suggest that FOXO3a plays a role in muscle atrophy through regulation of expression of MuRF1 and atrogin-1 in skeletal muscle in Cushing's syndrome. (FOXO3a = forkhead box protein O3a; MuRF1 = muscle-specific RING finger protein 1)
Title: Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.
Independent of the metabolic condition, after muscle contraction, both TRIM63 and FBXO32 proteins correlated significantly with miR-1-3p, miR-29a/b-3p, and miR-133a/b-3p.
Title: Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.

Submit: New GeneRIF Correction See all GeneRIFs (37)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables titin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to dexamethasone stimulus	ISO Inferred from Sequence Orthology more info
involved_in innate immune response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within muscle contraction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within myotube cell development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of glycolytic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in response to denervation involved in regulation of muscle adaptation	ISO Inferred from Sequence Orthology more info
involved_in response to electrical stimulus involved in regulation of muscle adaptation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to interleukin-1	IEP Inferred from Expression Pattern more info	PubMed
involved_in skeletal muscle atrophy	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of Cul4-RING E3 ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
located_in M band	IEA Inferred from Electronic Annotation more info
located_in Z disc	IEA Inferred from Electronic Annotation more info
located_in contractile muscle fiber	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM63

Names: MURF-1; RING-type E3 ubiquitin transferase TRIM63; muscle ring finger protein 1; muscle-specific RING finger protein 1; ring finger protein 28; tripartite motif containing 63, E3 ubiquitin protein ligase; tripartite motif protein 63; tripartite motif-containing protein 63

NP_543179.1

EC 2.3.2.27

XP_038965120.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080903.2 → NP_543179.1 E3 ubiquitin-protein ligase TRIM63

See identical proteins and their annotated locations for NP_543179.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

UniProtKB/Swiss-Prot

Q91Z63

UniProtKB/TrEMBL

A0A8I5ZNP7, A6IT16

Conserved Domains (3) summary

cd00162
Location:23 → 82

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

pfam00643
Location:120 → 159

zf-B_box; B-box zinc finger

cl23765
Location:166 → 283

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086023.1 Reference GRCr8

Range

151817209..151831026

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039109192.2 → XP_038965120.1 E3 ubiquitin-protein ligase TRIM63 isoform X1

UniProtKB/TrEMBL

A0A8I5ZNP7

Related

ENSRNOP00000058869.2, ENSRNOT00000067524.5

Conserved Domains (3) summary

smart00502
Location:166 → 283

BBC; B-Box C-terminal domain

cd16759
Location:18 → 80

RING-HC_MuRF1; RING finger, HC subclass, found in muscle-specific RING finger protein 1 (MuRF-1) and similar proteins

cd19831
Location:118 → 160

Bbox2_MuRF1_C-II; B-box-type 2 zinc finger found in muscle-specific RING finger protein 1 (MuRF-1) and similar proteins