VPS34 vacuolar protein sorting 34 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: VPS34
Official Full Name: vacuolar protein sorting 34
Primary source: TAIR:AT1G60490
Locus tag: AT1G60490
See related: Araport:AT1G60490
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATVPS34; F8A5.4; F8A5_4; PHOSPATIDYLINOSITOL 3-KINASE; PHOSPHATIDYLINOSITOL 3-KINASE; PI3K; vacuolar protein sorting 34
Summary: Encodes a phosphatidylinositol 3-kinase that is expressed in most plant tissues. Defects in VPS34 affect a number of cellular processes. Loss of function mutations are not transmitted through the male gametophyte due to defects in microgametogenesis therefore it is difficult to assess the effects of loss of VPS34 function in the whole plant. Involved in salt-stress responses.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See VPS34 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 17

Sequence:: Chromosome: 1; NC_003070.9 (22285572..22290389, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that phosphatidylinositol 3-kinase (PI3K) promotes vacuolar H(+)-ATPase (V-ATPase) B2 subunit activation and consequently induces vacuolar acidification and stomatal closure, thereby delaying methyl jasmonate (MeJA)-induced leaf senescence.
Title: Phosphatidylinositol 3-Kinase Promotes Activation and Vacuolar Acidification and Delays Methyl Jasmonate-Induced Leaf Senescence.
PI4K and PI3K are required for distinct steps in secretory and/or endocytic trafficking of CESA3.
Title: Phosphatidylinositol 3-kinase and 4-kinase have distinct roles in intracellular trafficking of cellulose synthase complexes in Arabidopsis thaliana.
Data demonstrated that AtVPS15 might interact directly with AtVPS34 and suggested that AtVPS15 is very important for pollen germination, possibly through modulation of the activity of PI3-kinase.
Title: Arabidopsis AtVPS15 plays essential roles in pollen germination possibly by interacting with AtVPS34.
Phosphatidylinositol 3-kinase is essential for vacuole reorganization and nuclear division during pollen development.
Title: The Arabidopsis phosphatidylinositol 3-kinase is important for pollen development.
Both endocytosis and ROS production were suppressed in phosphatidylinositol 3-kinase (PI3K) mutants, PI3K being a key regulator of vesicle trafficking in animals and plants, and by wortmannin, which is a specific inhibitor of PI3K.
Title: Induction of phosphatidylinositol 3-kinase-mediated endocytosis by salt stress leads to intracellular production of reactive oxygen species and salt tolerance.
activation of PtdIns 3-kinase and its product PtdIns(3)P are required for auxin-induced production of ROS and root gravitropism
Title: Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3-kinase.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

MASC_STS14446 (e-PCR)
- UniSTS:258714

NM_104735.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within endocytosis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within microgametogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of amino acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proline metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_positive_effect regulation of stomatal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to salicylic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to salt	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to salt stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISM Inferred from Sequence Model more info
part_of phosphatidylinositol 3-kinase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: vacuolar protein sorting 34

NP_176251.1

vacuolar protein sorting 34 (VPS34); FUNCTIONS IN: 1-phosphatidylinositol-3-kinase activity, inositol or phosphatidylinositol kinase activity, binding, phosphotransferase activity, alcohol group as acceptor; INVOLVED IN: oxygen and reactive oxygen species metabolic process, response to salt stress, endocytosis, N-terminal protein myristoylation, microgametogenesis; LOCATED IN: phosphoinositide 3-kinase complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Phosphatidylinositol 3-/4-kinase, catalytic (InterPro:IPR000403), Phosphoinositide 3-kinase, accessory (PIK) domain (InterPro:IPR001263), Phosphoinositide 3-kinase, C2 (InterPro:IPR002420), C2 calcium/lipid-binding domain, CaLB (InterPro:IPR008973), Protein kinase-like domain (InterPro:IPR011009), Phosphatidylinositol Kinase (InterPro:IPR015433), Phosphatidylinositol 3-kinase, Vps34 type (InterPro:IPR008290), Armadillo-type fold (InterPro:IPR016024), Phosphatidylinositol 3/4-kinase, conserved site (InterPro:IPR018936); BEST Arabidopsis thaliana protein match is: Phosphatidylinositol 3- and 4-kinase family protein (TAIR:AT1G49340.1); Has 3550 Blast hits to 3392 proteins in 278 species: Archae - 0; Bacteria - 0; Metazoa - 1514; Fungi - 819; Plants - 335; Viruses - 4; Other Eukaryotes - 878 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

22285572..22290389 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_104735.4 → NP_176251.1 vacuolar protein sorting 34 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_176251.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22695, P42339

UniProtKB/TrEMBL

A0A5S9WQW4, A0A654EJR0

Conserved Domains (3) summary

cd08397
Location:25 → 178

C2_PI3K_class_III; C2 domain present in class III phosphatidylinositol 3-kinases (PI3Ks)

cd00870
Location:274 → 432

PI3Ka_III; Phosphoinositide 3-kinase (PI3K) class III, accessory domain (PIK domain); PIK domain is conserved in all PI3 and PI4-kinases. Its role is unclear but it has been suggested to be involved in substrate presentation. In general, PI3Ks class III ...

cd00896
Location:466 → 811

PI3Kc_III; Catalytic domain of Class III Phosphoinositide 3-kinase