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    AAT aspartate aminotransferase [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 816758, updated on 18-Sep-2024

    Summary

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    Official Symbol
    AAT
    Official Full Name
    aspartate aminotransferase
    Primary source
    TAIR:AT2G22250
    Locus tag
    AT2G22250
    See related
    Araport:AT2G22250
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    aspartate aminotransferase; ASPARTATE AMINOTRANSFERASE; ATAAT; MATERNAL EFFECT EMBRYO ARREST 17; MEE17; T26C19.9; T26C19_9
    Summary
    Encodes a prokaryotic-type plastidic aspartate aminotransferase with glutamate/aspartate-prephenate aminotransferase (PAT) activity.
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    Genomic context

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    See AAT in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    11
    Sequence:
    Chromosome: 2; NC_003071.7 (9457786..9460919, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Thioesterase superfamily protein Neighboring gene myo-inositol-1-phosphate synthase 2 Neighboring gene uncharacterized protein Neighboring gene oxidoreductase, 2OG-Fe(II) oxygenase family protein Neighboring gene hematological/neurological-like protein Neighboring gene tRNA-Arg

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Prephenate aminotransferase directs plant phenylalanine biosynthesis via arogenate. Maeda H, et al. Nat Chem Biol, 2011 Jan. PMID 21102469
    2. Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis. Graindorge M, et al. FEBS Lett, 2010 Oct 22. PMID 20883697
    3. Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase. Giustini C, et al. FEBS J, 2019 Jun. PMID 30771275
    4. Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis. Pagnussat GC, et al. Development, 2005 Feb. PMID 15634699
    5. Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism. Ito J, et al. J Proteome Res, 2011 Apr 1. PMID 21166475

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. X-ray crystal structure analysis supported by site-directed mutagenesis, modeling, and molecular dynamics simulations identified a molecular determinant of prephenate aminotransferase activity in the flexible N-terminal loop of 1beta AAT. A Lys/Arg/Gln residue in position 12 in the sequence present only in PAT-competent enzymes could interact with the 4-hydroxyl group of the prephenate substrate.
      Title: Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase.
    2. Data show that the purifies prephenate aminotransferase activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250).
      Title: Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001036317.1
    NM_127791.3
    NM_179691.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables L-aspartate:2-oxoglutarate aminotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aspartate-prephenate aminotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glutamate-prephenate aminotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-phenylalanine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within aromatic amino acid family biosynthetic process, prephenate pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in aromatic amino acid family biosynthetic process, prephenate pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within embryo development ending in seed dormancy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast stroma HDA PubMed 
    NOT located_in cytosol RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    aspartate aminotransferase
    NP_001031394.1
    • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, transferase activity, transferring nitrogenous groups, pyridoxal phosphate binding, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_565529.1
    • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, transferase activity, transferring nitrogenous groups, pyridoxal phosphate binding, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 50315 Blast hits to 50311 proteins in 3104 species: Archae - 1094; Bacteria - 34657; Metazoa - 783; Fungi - 895; Plants - 1393; Viruses - 0; Other Eukaryotes - 11493 (source: NCBI BLink).
    NP_850022.1
    • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, pyridoxal phosphate binding, transferase activity, transferring nitrogenous groups, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 49853 Blast hits to 49849 proteins in 3081 species: Archae - 1094; Bacteria - 34200; Metazoa - 783; Fungi - 895; Plants - 1391; Viruses - 0; Other Eukaryotes - 11490 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      9457786..9460919 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_179691.4 → NP_850022.1  aspartate aminotransferase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850022.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178VZ85
      Conserved Domains (2) summary
      cd00609
      Location:55 → 417
      AAT_like; Aspartate aminotransferase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Pyridoxal phosphate combines with an alpha-amino acid to form a compound called a Schiff base or aldimine ...
      PRK05764
      Location:23 → 423
      PRK05764; aspartate aminotransferase; Provisional

    2. NM_127791.3 → NP_565529.1  aspartate aminotransferase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_565529.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      E9L7A7, Q3E6N9, Q8LFX1, Q8VZI1, Q9SIE1
      UniProtKB/TrEMBL
      A0A178VZ85, A0A5S9X058, A0A654EW86
      Conserved Domains (1) summary
      PRK05764
      Location:70 → 470
      PRK05764; aspartate aminotransferase; Provisional

    3. NM_001036317.1 → NP_001031394.1  aspartate aminotransferase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001031394.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      E9L7A7, Q3E6N9, Q8LFX1, Q8VZI1, Q9SIE1
      UniProtKB/TrEMBL
      A0A178VZ85, A0A5S9X058, A0A654EW86
      Conserved Domains (1) summary
      PRK05764
      Location:70 → 470
      PRK05764; aspartate aminotransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SIE1.2

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