JAR1 Auxin-responsive GH3 family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: JAR1
Official Full Name: Auxin-responsive GH3 family protein
Primary source: TAIR:AT2G46370
Locus tag: AT2G46370
See related: Araport:AT2G46370
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtGH3.11; F11C10.6; FAR-RED INSENSITIVE 219; FIN219; JASMONATE RESISTANT 1
Summary: Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.
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Genomic context

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See JAR1 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 5

Sequence:: Chromosome: 2; NC_003071.7 (19033586..19036683)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

FAR-RED INSENSITIVE 219 and phytochrome B corepress shade avoidance via modulating nuclear speckle formation.
Title: FAR-RED INSENSITIVE 219 and phytochrome B corepress shade avoidance via modulating nuclear speckle formation.
JASMONATE RESISTANT 1 negatively regulates root growth under boron deficiency in Arabidopsis.
Title: JASMONATE RESISTANT 1 negatively regulates root growth under boron deficiency in Arabidopsis.
FIN219/JAR1 and CRY1 antagonize each other to modulate photomorphogenic development of seedlings and stress responses in Arabidopsis.FIN219 and CRY1 negatively regulated each other by direct interaction in response to jasmonate under blue light.
Title: FIN219/JAR1 and cryptochrome1 antagonize each other to modulate photomorphogenesis under blue light in Arabidopsis.
We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219-FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding.
Title: Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
FIN219/JAR1 is tightly regulated in response to exogenous jasmonic acid.
Title: Far-Red Light-Mediated Seedling Development in Arabidopsis Involves FAR-RED INSENSITIVE 219/JASMONATE RESISTANT 1-Dependent and -Independent Pathways.
crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1
Title: Structural basis for prereceptor modulation of plant hormones by GH3 proteins.
The regulation of COP1 by FIN219, was studied.
Title: FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis.
Increase in jasmonic acid-isoleucine mediated by the JAR1 enzyme plays only a minor role in transcriptional modulation of genes induced by mechanical wounding.
Title: The role of JAR1 in Jasmonoyl-L: -isoleucine production during Arabidopsis wound response.
FIN219 interacts with FIP1 to regulate cell elongation and flowering in response to light.
Title: Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis.
analyzed the substrate specificity of recombinant JAR1 and determined whether it catalyzes the synthesis of mono- and dinucleoside polyphosphates
Title: Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1).

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001084600.1

Supported by ESTs:

NM_001202828.1

Supported by ESTs:

NM_130200.2

Supported by ESTs:

NM_180122.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables L-leucine binding	IDA Inferred from Direct Assay more info	PubMed
enables adenylyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables amino acid binding	IDA Inferred from Direct Assay more info	PubMed
enables catalytic activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables jasmonoyl-L-amino acid ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables jasmonoyl-L-amino acid ligase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to auxin stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within induced systemic resistance, jasmonic acid mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in induced systemic resistance, jasmonic acid mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within jasmonic acid and ethylene-dependent systemic resistance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within jasmonic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within jasmonic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of defense response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within photomorphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein adenylylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within red, far-red light phototransduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of response to red or far red light	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to UV-B	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to jasmonic acid	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to mycotoxin	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to ozone	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to wounding	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within systemic acquired resistance	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
located_in vacuole	HDA more info	PubMed

General protein information

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Preferred Names: Auxin-responsive GH3 family protein

NP_001078069.1

JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1356 Blast hits to 1352 proteins in 227 species: Archae - 0; Bacteria - 427; Metazoa - 52; Fungi - 2; Plants - 657; Viruses - 0; Other Eukaryotes - 218 (source: NCBI BLink).

NP_001189757.1

JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1).

NP_566071.1

JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).

NP_850453.1

JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

19033586..19036683

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_180122.4 → NP_850453.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850453.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C0Z3D1, Q42147, Q56YY1, Q949V9, Q9LKI2, Q9SKE2

UniProtKB/TrEMBL

A0A178VQG1, A0A5S9X7G2, A0A7G2EKT2

Conserved Domains (1) summary

pfam03321
Location:16 → 555

GH3; GH3 auxin-responsive promoter
NM_001202828.1 → NP_001189757.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189757.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2EKT2, F4II77

Conserved Domains (2) summary

cl21606
Location:20 → 583

PLN02247; indole-3-acetic acid-amido synthetase

pfam03321
Location:27 → 566

GH3; GH3 auxin-responsive promoter
NM_130200.2 → NP_566071.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_566071.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C0Z3D1, Q42147, Q56YY1, Q949V9, Q9LKI2, Q9SKE2

UniProtKB/TrEMBL

A0A178VQG1, A0A5S9X7G2, A0A7G2EKT2

Conserved Domains (1) summary

pfam03321
Location:16 → 555

GH3; GH3 auxin-responsive promoter
NM_001084600.1 → NP_001078069.1 Auxin-responsive GH3 family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078069.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2EKT2

Conserved Domains (2) summary

cl21606
Location:3 → 496

PLN02247; indole-3-acetic acid-amido synthetase

pfam03321
Location:1 → 476

GH3; GH3 auxin-responsive promoter