NRAMP4 natural resistance associated macrophage protein 4 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NRAMP4
Official Full Name: natural resistance associated macrophage protein 4
Primary source: TAIR:AT5G67330
Locus tag: AT5G67330
See related: Araport:AT5G67330
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 4; ATNRAMP4; K8K14.5; K8K14_5; METAL TRANSPORTER NRAMP4; natural resistance associated macrophage protein 4
Summary: Encodes a member of the Nramp2 metal transporter family; like its homolog Atnramp3, localized in vacuolar membrane. Seedlings of double mutant, atnramp3-1 atnramp4-1, were arrested at early germination.
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Genomic context

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See NRAMP4 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 3

Sequence:: Chromosome: 5; NC_003076.8 (26861266..26863897)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NRAMP4 expression in the germinating seeds and its role in iron metabolism.
Title: Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds.
Sorting of NRAMP4 depends on N-terminal dileucine-based motif and adaptor protein complex AP4.
Title: Sorting of Arabidopsis NRAMP3 and NRAMP4 depends on adaptor protein complex AP4 and a dileucine-based motif.
analysis of subcellular Fe localization indicates that simultaneous disruption of AtVIT1, AtNRAMP3, and AtNRAMP4 limits Fe accumulation in vacuolar globoids.
Title: Bypassing Iron Storage in Endodermal Vacuoles Rescues the Iron Mobilization Defect in the natural resistance associated-macrophage protein3natural resistance associated-macrophage protein4 Double Mutant.
The Cadmium (Cd) hypersensitivity of nramp3nramp4 mutant highlights the critical role of vacuolar metal stores to supply essential metals to plastids and maintain photosynthetic function under Cd and oxidative stresses.
Title: Mutants impaired in vacuolar metal mobilization identify chloroplasts as a target for cadmium hypersensitivity in Arabidopsis thaliana.
results presented here suggest an important role for AtNRAMP3/AtNRAMP4-dependent Mn transit through the vacuole prior to the import into chloroplasts of mesophyll cells
Title: Export of vacuolar manganese by AtNRAMP3 and AtNRAMP4 is required for optimal photosynthesis and growth under manganese deficiency.
AtNRAMP3 and AtNRAMP4 are involved in Arabidopsis thaliana resistance against the bacterial pathogen E. chrysanthemi.
Title: NRAMP genes function in Arabidopsis thaliana resistance to Erwinia chrysanthemi infection.
Mobilization of vacuolar iron (Fe) stores by AtNRAMP4 is crucial to support Arabidopsis early development until efficient systems for Fe acquisition from the soil take over.
Title: Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_126133.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables inorganic anion transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables manganese ion transmembrane transporter activity	TAS Traceable Author Statement more info	PubMed
enables metal ion transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables metal ion transmembrane transporter activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cadmium ion transport	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular iron ion homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within intracellular monoatomic cation homeostasis	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within lead ion transport	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within manganese ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within manganese ion transport	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within metal ion transport	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within multicellular organismal-level iron ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of reactive oxygen species metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to iron ion	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to nematode	HEP more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in plant-type vacuole	HDA more info	PubMed
located_in plastid	HDA more info	PubMed
located_in vacuolar membrane	IDA Inferred from Direct Assay more info	PubMed
located_in vacuole	HDA more info	PubMed

General protein information

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Preferred Names: natural resistance associated macrophage protein 4

NP_201534.1

natural resistance associated macrophage protein 4 (NRAMP4); FUNCTIONS IN: manganese ion transmembrane transporter activity, inorganic anion transmembrane transporter activity, metal ion transmembrane transporter activity; INVOLVED IN: in 11 processes; LOCATED IN: vacuolar membrane, vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Natural resistance-associated macrophage protein (InterPro:IPR001046); BEST Arabidopsis thaliana protein match is: natural resistance-associated macrophage protein 3 (TAIR:AT2G23150.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

26861266..26863897

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_126133.4 → NP_201534.1 natural resistance associated macrophage protein 4 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_201534.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WWH0, Q9FN18, Q9SNV8

UniProtKB/TrEMBL

A0A178UJ02, A0A5S9YI31

Conserved Domains (1) summary

cl00456
Location:50 → 428

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain