CHAF1B chromatin assembly factor 1 subunit B [Homo sapiens (human)] - Gene

Summary

Official Symbol: CHAF1Bprovided by HGNC
Official Full Name: chromatin assembly factor 1 subunit Bprovided by HGNC
Primary source: HGNC:HGNC:1911
See related: Ensembl:ENSG00000159259 MIM:601245; AllianceGenome:HGNC:1911
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
Summary: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Expression: Broad expression in bone marrow (RPKM 2.7), testis (RPKM 2.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 21q22.12-q22.13

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	21	NC_000021.9 (36385392..36419015)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	21	NC_060945.1 (34767975..34801594)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	21	NC_000021.8 (37757690..37791313)

Chromosome 21 - NC_000021.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells.
Title: Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells.
The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.
Title: The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.
CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
Title: CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
Study revealed that CHAF1B was highly expressed in nonsmall cell lung cancer (NSCLC) lung tissues and 95D cells. Survival analysis indicated that high CHAF1B expression in tumor tissue was associated with poor clinical outcomes in NSCLC patients. CHAF1B knockdown in 95D cells markedly inhibited tumor proliferation, reduced colony formation, induced cell cycle arrest and promoted apoptosis.
Title: CHAF1B promotes proliferation and reduces apoptosis in 95‑D lung cancer cells and predicts a poor prognosis in non‑small cell lung cancer.
The volumes of the tumors were similar between those injected with the empty vector and control, but were significantly smaller in the knockdown models, suggesting that the knockdown of the CHAF1B gene inhibited tumor growth. H&E staining revealed that tumors were developed in mice in all groups.
Title: CHAF1B knockdown blocks migration in a hepatocellular carcinoma model.
All the evaluated malignant tumors showed CAF-1 p60 overexpression, confirming the emerging role of CAF-1 p60 as a new proliferation and prognostic marker for cancer.
Title: Tissue microarray-based evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as tumour prognostic marker.
The levels of the EDNRB, HJURP and p60/CAF-1 proteins were strongly associated with overall survival in high-grade gliomas patients (p<0.001, p<0.001 and p=0.002, respectively), whereas the one of PDLI4 was not (P=0.11).
Title: Prognostic significance of EDN/RB, HJURP, p60/CAF-1 and PDLI4, four new markers in high-grade gliomas.
Overexpression of CAF-1/p60, on histological and/or cytological samples, characterizes malignant salivary gland tumours with aggressive behaviour
Title: The proliferation marker Chromatin Assembly Factor-1 is of clinical value in predicting the biological behaviour of salivary gland tumours.
CAF-1 is a proliferation marker in various malignant tumours with prognostic value in renal, endometrial and cervical carcinomas, which supports the value of CAF-1 as a clinical marker of cancer progression.
Title: Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours.
Chromatin Assembly Factor-1/p60 may have a role in progression and metastasis of melanoma
Title: Overexpression of Chromatin Assembly Factor-1/p60 helps to predict the prognosis of melanoma patients.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of lung function phenotypes in a founder population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G34657 (e-PCR)
- UniSTS:78156

SHGC-51966 (e-PCR)
- UniSTS:5977

D21S1939 (e-PCR)
- UniSTS:63591

SHGC-9552 (e-PCR)
- UniSTS:48513

STS-U20980 (e-PCR)
- UniSTS:9190

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables chromatin binding	TAS Traceable Author Statement more info	PubMed
enables histone binding	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables unfolded protein binding	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication-dependent chromatin assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleosome assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of CAF-1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CAF-1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of CAF-1 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	NAS Non-traceable Author Statement more info	PubMed
colocalizes_with cytosol	HDA more info	PubMed
colocalizes_with nucleoplasm	HDA more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: chromatin assembly factor 1 subunit B

Names: CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7; chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_046962.1 RefSeqGene

Range

13335..44771

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_005441.3 → NP_005432.1 chromatin assembly factor 1 subunit B

See identical proteins and their annotated locations for NP_005432.1

Status: REVIEWED

Source sequence(s)

AL711314, AP000694, BC001143, BC021218

Consensus CDS

CCDS13644.1

UniProtKB/Swiss-Prot

Q13112, Q99548

UniProtKB/TrEMBL

B2R7X3

Related

ENSP00000315700.4, ENST00000314103.6

Conserved Domains (3) summary

sd00039
Location:16 → 64

7WD40; WD40 repeat [structural motif]

pfam15512
Location:381 → 555

CAF-1_p60_C; Chromatin assembly factor complex 1 subunit p60, C-terminal

cl29593
Location:13 → 251

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...