Prep prolyl endopeptidase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prepprovided by MGI
Official Full Name: prolyl endopeptidaseprovided by MGI
Primary source: MGI:MGI:1270863
See related: Ensembl:ENSMUSG00000019849 AllianceGenome:MGI:1270863
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PE; PEP; Pop; D10Wsu136e
Summary: Enables metallocarboxypeptidase activity. Involved in angiotensin maturation. Located in cytoplasm and nucleus. Is active in extracellular region. Is expressed in several structures, including cranial ganglion; genitourinary system; heart ventricle; thymus primordium; and vibrissa. Orthologous to human PREP (prolyl endopeptidase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 28.1), placenta adult (RPKM 26.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B2; 10 23.27 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (44943312..45038847)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (45067216..45162751)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
POP regulates progesterone secretion by stimulating the expression of CYP11A, 3beta-HSD, and StAR in luteal cells. ERK signaling and downstream SF1 expression contribute to this process.
Title: Prolyl oligopeptidase regulates progesterone secretion via the ERK signaling pathway in murine luteal cells.
Results strongly suggest that there is a pivotal role for Prolyl endopeptidase (PREP) in vesicle exocytosis, as well as in the physiology of mouse seminal vesicles.
Title: Study on PREP localization in mouse seminal vesicles and its possible involvement during regulated exocytosis.
Prolyl oligopeptidase expression and role in the placental spongiotrophoblast differentiation.
Title: A molecular mechanism of mouse placental spongiotrophoblast differentiation regulated by prolyl oligopeptidase.
PREP regulates the function of dopamine transporter, possibly by controlling the phosphorylation and transport of dopamine transporter into the striatum or synaptic membrane.
Title: Prolyl Oligopeptidase Regulates Dopamine Transporter Phosphorylation in the Nigrostriatal Pathway of Mouse.
Taken together, these data suggest that PREP can enhance alpha-synuclein toxicity in vivo.
Title: Removal of prolyl oligopeptidase reduces alpha-synuclein toxicity in cells and in vivo.
involved in trophoblast stem cell differentiation in placenta
Title: Mouse prolyl oligopeptidase plays a role in trophoblast stem cell differentiation into trophoblast giant cell and spongiotrophoblast.
These findings might be explained as possible alteration in brain plasticity caused by PREP deficiency, which in turn affect behaviour and brain development
Title: Deficiency of prolyl oligopeptidase in mice disturbs synaptic plasticity and reduces anxiety-like behaviour, body weight, and brain volume.
Lung ischemia-reperfusion injury causes a dysregulation of circulating Ang 2 levels and plasma PREP activity, although no direct link between both phenomena could be shown.
Title: Dysregulation of the renin-angiotensin system during lung ischemia-reperfusion injury.
TLR4 activation releases prolyl endopeptidase containing exosomes from airway epithelial cells.
Title: Toll-Like Receptor 4 Engagement Mediates Prolyl Endopeptidase Release from Airway Epithelia via Exosomes.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Gene trapped (1)
Targeted (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI450383 (e-PCR)
- UniSTS:165689

C87926 (e-PCR)
- UniSTS:208517

RH135835 (e-PCR)
- UniSTS:209833

RH124895 (e-PCR)
- UniSTS:161717

MHAa28h6.seq (e-PCR)
- UniSTS:125113

D10Wsu136e (e-PCR), detects polymorphism
- UniSTS:141524

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallocarboxypeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables oligopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables peptidase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables peptide binding	ISO Inferred from Sequence Orthology more info
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in angiotensin maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein metabolic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: prolyl endopeptidase

Names: post-proline cleaving enzyme; prolyl oligopeptidase

NP_035286.1

EC 3.4.21.26

XP_011241444.1

EC 3.4.21.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011156.3 → NP_035286.1 prolyl endopeptidase

See identical proteins and their annotated locations for NP_035286.1

Status: VALIDATED

Source sequence(s)

AC153535, AK076257, AK163774, BP759584

Consensus CDS

CCDS23826.1

UniProtKB/Swiss-Prot

Q80YS1, Q9QUR6

UniProtKB/TrEMBL

Q3TCS0, Q543B9

Related

ENSMUSP00000097444.3, ENSMUST00000099858.4

Conserved Domains (2) summary

COG1505
Location:26 → 704

PreP; Prolyl oligopeptidase PreP, S9A serine peptidase family [Amino acid transport and metabolism]

pfam00326
Location:482 → 707

Peptidase_S9; Prolyl oligopeptidase family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

44943312..45038847

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011243142.3 → XP_011241444.1 prolyl endopeptidase isoform X1

Conserved Domains (1) summary

COG1505
Location:9 → 477

PreP; Prolyl oligopeptidase PreP, S9A serine peptidase family [Amino acid transport and metabolism]