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    TH1 thiamine biosynthesis protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 838901, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TH1
    Official Full Name
    thiamine biosynthesis protein
    Primary source
    TAIR:AT1G22940
    Locus tag
    AT1G22940
    See related
    Araport:AT1G22940
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    F19G10.10; F19G10_10; TH-1; THIAMINE REQUIRING 1; THIAMINEE; THIE
    Summary
    Encodes a bifunctional enzyme required for thiamine (vitamin B1) biosynthesis. TH1 can phosphorylate HMP-P to produce HMP-PP, the pyrimidine heterocyclic subunit of thiamine. TH1 also catalyzes the condensation of HMP-PP and HET to form thiamine monophosphate (TMP). TH1 also appears capable of phosphorylating HMP based on E.coli mutant complementation assays. th1 mutants are thiamine auxotrophs that die as seedlings on unsupplemented media.
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    Genomic context

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    See TH1 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    8
    Sequence:
    Chromosome: 1; NC_003070.9 (8122339..8125068)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene COP9 signalosome 5A Neighboring gene T-complex protein 11 Neighboring gene 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein Neighboring gene Pentatricopeptide repeat (PPR) superfamily protein Neighboring gene cyclin-D1-binding protein Neighboring gene Grap2/cyclin-D-interacting protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Determination of the genetic, molecular, and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1. Ajjawi I, et al. Arch Biochem Biophys, 2007 Mar 1. PMID 17174261
    2. A th-1 Mutant of Arabidopsis thaliana Is Defective for a Thiamin-Phosphate-Synthesizing Enzyme: Thiamin Phosphate Pyrophosphorylase. Komeda Y, et al. Plant Physiol, 1988 Oct. PMID 16666289, Free PMC Article
    3. Functional characterization of the thi1 promoter region from Arabidopsis thaliana. Ribeiro DT, et al. J Exp Bot, 2005 Jul. PMID 15897230
    4. Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants. Strobbe S, et al. Plant Physiol, 2021 Aug 3. PMID 33944954, Free PMC Article
    5. AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins. Ferro M, et al. Mol Cell Proteomics, 2010 Jun. PMID 20061580, Free PMC Article

    See all (7) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
      Title: Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
    2. This study demonstrated that expression derived from the thi1 promoter is detected early on during development and continues throughout the plant's life cycle.
      Title: Functional characterization of the thi1 promoter region from Arabidopsis thaliana.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    NM_102141.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables hydroxymethylpyrimidine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydroxymethylpyrimidine kinase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables phosphomethylpyrimidine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphomethylpyrimidine kinase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables thiamine-phosphate diphosphorylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables thiamine-phosphate diphosphorylase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within thiamine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast stroma HDA PubMed 

    General protein information

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    Preferred Names
    thiamine biosynthesis protein
    NP_173707.2
    • THIAMINE REQUIRING 1 (TH1); FUNCTIONS IN: phosphomethylpyrimidine kinase activity, thiamin-phosphate diphosphorylase activity, hydroxymethylpyrimidine kinase activity; INVOLVED IN: thiamin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine monophosphate synthase (InterPro:IPR003733), Phosphomethylpyrimidine kinase type-2 (InterPro:IPR004399), Aldolase-type TIM barrel (InterPro:IPR013785), Phosphomethylpyrimidine kinase type-1 (InterPro:IPR013749); Has 15989 Blast hits to 15891 proteins in 2548 species: Archae - 338; Bacteria - 12149; Metazoa - 165; Fungi - 331; Plants - 107; Viruses - 0; Other Eukaryotes - 2899 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      8122339..8125068
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_102141.3 → NP_173707.2  thiamine biosynthesis protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_173707.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O23128, Q5M731, Q8L7M9
      UniProtKB/TrEMBL
      A0A178W9W3, A0A5S9VMW4, A0A7G2DXI6
      Conserved Domains (1) summary
      PLN02898
      Location:22 → 522
      PLN02898; HMP-P kinase/thiamin-monophosphate pyrophosphorylase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q5M731.1

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