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    AVP1 Inorganic H pyrophosphatase family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 838138, updated on 18-Sep-2024

    Summary

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    Official Symbol
    AVP1
    Official Full Name
    Inorganic H pyrophosphatase family protein
    Primary source
    TAIR:AT1G15690
    Locus tag
    AT1G15690
    See related
    Araport:AT1G15690
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ARABIDOPSIS THALIANA V-PPASE 3; AtAVP1; ATAVP3; AtVHP1;1; AVP-3; F7H2.3; F7H2_3; FUGU 5; FUGU5; VACUOLAR H+ - PYROPHOSPHATASE AVP-3; VHP1
    Summary
    Encodes a H(+)-translocating (pyrophosphate-energized) inorganic pyrophosphatase (H(+)-PPase; EC 3.6.1.1) located in the vacuolar membrane. Expression is found in all tissues examined, including meristems and floral organ primordium. Expression is particularly enhanced in pollen, and is repressed by light. Over expression and loss of function phenotypes suggest AVP1 is involved in regulation of apoplastic pH and auxin transport. The effect on auxin transport likely involves effects of extracellular pH on subcellular localization of auxin efflux carriers such as PIN1.
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    Genomic context

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    See AVP1 in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    8
    Sequence:
    Chromosome: 1; NC_003070.9 (5398926..5402733)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene F-box family protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ATPase, F1 complex, gamma subunit protein Neighboring gene prephenate dehydrogenase family protein Neighboring gene ncRNA Neighboring gene TRF-like 5

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Keep an eye on PPi: the vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis. Ferjani A, et al. Plant Cell, 2011 Aug. PMID 21862707, Free PMC Article
    2. Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana. Sarafian V, et al. Proc Natl Acad Sci U S A, 1992 Mar 1. PMID 1311852, Free PMC Article
    3. Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Gaxiola RA, et al. Proc Natl Acad Sci U S A, 2001 Sep 25. PMID 11572991, Free PMC Article
    4. Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development. Li J, et al. Science, 2005 Oct 7. PMID 16210544
    5. Enhanced proton translocating pyrophosphatase activity improves nitrogen use efficiency in Romaine lettuce. Paez-Valencia J, et al. Plant Physiol, 2013 Mar. PMID 23307651, Free PMC Article

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Improving drought-, salinity-, and heat-tolerance in transgenic plants by co-overexpressing Arabidopsis vacuolar pyrophosphatase gene AVP1 and Larrea Rubisco activase gene RCA.
      Title: Improving drought-, salinity-, and heat-tolerance in transgenic plants by co-overexpressing Arabidopsis vacuolar pyrophosphatase gene AVP1 and Larrea Rubisco activase gene RCA.
    2. AVP1 is required for cellular pyrophosphate homeostasis that in turn contributes to high-Magnesium tolerance in plant cells.
      Title: Vacuolar Proton Pyrophosphatase Is Required for High Magnesium Tolerance in Arabidopsis.
    3. Transgenic Arabidopsis plants expressing both AVP1 and PP2A-C5 at relatively high levels were identified and analyzed. These plants displayed enhanced tolerance to NaCl compared to either AVP1 or PP2A-C5 overexpressing plants.
      Title: Co-overexpression of AVP1 and PP2A-C5 in Arabidopsis makes plants tolerant to multiple abiotic stresses.
    4. AVP1 role in phloem loading and transport
      Title: Constitutive and Companion Cell-Specific Overexpression of AVP1, Encoding a Proton-Pumping Pyrophosphatase, Enhances Biomass Accumulation, Phloem Loading, and Long-Distance Transport.
    5. AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.
      Title: Arabidopsis type I proton-pumping pyrophosphatase expresses strongly in phloem, where it is required for pyrophosphate metabolism and photosynthate partitioning.
    6. AVP1DOX plants produced 25% more marketable ripened fruit per plant under P-deficient conditions compared with the controls. Further, under low phosphate conditions, they displayed increased phosphate transport from leaf (source) to fruit (sink).
      Title: Over-expression of the Arabidopsis proton-pyrophosphatase AVP1 enhances transplant survival, root mass, and fruit development under limiting phosphorus conditions.
    7. The expression of AVP1 increases the shoot biomass of barley in saline and nonsaline conditions. It is shown that the expression of AVP1 in transgenic barley improves the grain yield per plant of this cereal crop when grown in a high-salinity field.
      Title: Expression of the Arabidopsis vacuolar H⁺-pyrophosphatase gene (AVP1) improves the shoot biomass of transgenic barley and increases grain yield in a saline field.
    8. Results show that transgenic plants expressing higher levels of AVP1 transcripts in sugarcane are able to withstand salt and drought stress regimes.
      Title: Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase (AVP1) gene.
    9. The characterization of romaine lettuce (Lactuca sativa 'Conquistador') plants engineered to overexpress an intragenic gain-of-function allele of the type I proton translocating pyrophosphatase (H(+)-PPase) of Arabidopsis, is reported.
      Title: Enhanced proton translocating pyrophosphatase activity improves nitrogen use efficiency in Romaine lettuce.
    10. Hydrolysis of cytosolic PPi, rather than vacuolar acidification, is the major function of AVP1/FUGU5 in planta. Plant cells optimize their metabolic function by eliminating PPi in the cytosol for efficient postembryonic heterotrophic growth.
      Title: Keep an eye on PPi: the vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001084073.1
    NM_101437.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables diphosphate hydrolysis-driven proton transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables diphosphate hydrolysis-driven proton transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables inorganic diphosphate phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables inorganic diphosphate phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within auxin polar transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within cell wall pectin metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within establishment or maintenance of transmembrane electrochemical gradient TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within leaf development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of starch metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to salt stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to water deprivation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within sucrose metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus HDA PubMed 
    located_in chloroplast HDA PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast envelope HDA PubMed 
    NOT located_in cytosol RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    located_in endosome membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular region HDA PubMed 
    located_in mitochondrion HDA PubMed 
    located_in plant-type vacuole HDA PubMed 
    located_in plant-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane HDA PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in vacuolar membrane HDA PubMed 
    located_in vacuole HDA PubMed 

    General protein information

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    Preferred Names
    Inorganic H pyrophosphatase family protein
    NP_001077542.1
    • AVP1; FUNCTIONS IN: hydrogen-translocating pyrophosphatase activity; INVOLVED IN: response to water deprivation, response to salt stress, establishment or maintenance of transmembrane electrochemical gradient, leaf development, auxin polar transport; LOCATED IN: in 7 components; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Inorganic H+ pyrophosphatase (InterPro:IPR004131); BEST Arabidopsis thaliana protein match is: Inorganic H pyrophosphatase family protein (TAIR:AT1G16780.1); Has 5891 Blast hits to 5869 proteins in 792 species: Archae - 67; Bacteria - 1317; Metazoa - 5; Fungi - 0; Plants - 299; Viruses - 0; Other Eukaryotes - 4203 (source: NCBI BLink).
    NP_173021.1
    • AVP1; FUNCTIONS IN: hydrogen-translocating pyrophosphatase activity; INVOLVED IN: response to water deprivation, response to salt stress, establishment or maintenance of transmembrane electrochemical gradient, leaf development, auxin polar transport; LOCATED IN: in 10 components; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Inorganic H+ pyrophosphatase (InterPro:IPR004131); BEST Arabidopsis thaliana protein match is: Inorganic H pyrophosphatase family protein (TAIR:AT1G16780.1); Has 6470 Blast hits to 6448 proteins in 793 species: Archae - 67; Bacteria - 1322; Metazoa - 5; Fungi - 1; Plants - 304; Viruses - 0; Other Eukaryotes - 4771 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      5398926..5402733
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_101437.5 → NP_173021.1  Inorganic H pyrophosphatase family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_173021.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O80390, P31414, Q41919, Q41920, Q56WP6, Q8RY20, Q8VZE3
      UniProtKB/TrEMBL
      A0A178WA14, A0A5S9UN69, Q0WWI1
      Conserved Domains (1) summary
      PLN02255
      Location:4 → 770
      PLN02255; H(+) -translocating inorganic pyrophosphatase

    2. NM_001084073.1 → NP_001077542.1  Inorganic H pyrophosphatase family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001077542.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8MQH1, Q0WWI1
      Conserved Domains (2) summary
      PLN02255
      Location:4 → 633
      PLN02255; H(+) -translocating inorganic pyrophosphatase
      PRK00733
      Location:76 → 632
      hppA; membrane-bound proton-translocating pyrophosphatase; Validated
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P31414.1

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