APG8A Ubiquitin-like superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: APG8A
Official Full Name: Ubiquitin-like superfamily protein
Primary source: TAIR:AT4G21980
Locus tag: AT4G21980
See related: Araport:AT4G21980
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATG8A; AUTOPHAGY 8A; AUTOPHAGY-RELATED 8A; F1N20.80; F1N20_80
Summary: Encodes APG8, a component of autophagy conjugation pathway. Delivered to the lumens of vacuole under nitrogen-starvation condition. Highest expression in flowers. mRNA abundance increased during dark-induced carbon starvation. Predominantly cytoplasmic with or without N starvation. Upon concanamycin A the protein accumulates in the central vacuole as punctuate structures that resemble autophagic bodies. This localization is more abundant upon N starvation.
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Genomic context

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See APG8A in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 6

Sequence:: Chromosome: 4; NC_003075.7 (11655562..11657034)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

study shows that the overexpression of ATG8 genes provides beneficial effects on N management at the whole-plant level without affecting yield or plant biomass.
Title: Overexpression of ATG8 in Arabidopsis Stimulates Autophagic Activity and Increases Nitrogen Remobilization Efficiency and Grain Filling.
Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation, and autophagic flux. It also induces transcriptional changes opposite to those observed in atg5 and atg7 mutants, favoring stress resistance and growth.
Title: Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness.
we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine, and expression of a fusion between GFP and the autophagosomal membrane protein ATG8.
Title: Detection of Autophagy in Plants by Fluorescence Microscopy.
Here, we describe how ATG8 can be used to monitor autophagy in Chlamydomonas and Arabidopsis by western blot analysis
Title: Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8.
Data indicate that proteasome subunit RPN10 acts as a proteaphagy receptor by binding ubiquitylated proteasomes and autophagy-related protein ATG8.
Title: Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis.
The formation of the ATG12-ATG5 adduct is essential for ATG8-mediated autophagy in plants by promoting ATG8 lipidation.
Title: ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001084955.1

Supported by ESTs:

NM_118319.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables Atg8 activating enzyme activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables Atg8-family ligase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-phosphatidylethanolamide deconjugating activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within autophagy	ISS Inferred from Sequence or Structural Similarity more info	PubMed
acts_upstream_of_or_within defense response to fungus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome	IPI Inferred from Physical Interaction more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	NAS Non-traceable Author Statement more info
located_in phagophore	IPI Inferred from Physical Interaction more info	PubMed
located_in vacuolar lumen	NAS Non-traceable Author Statement more info

General protein information

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Preferred Names: Ubiquitin-like superfamily protein

NP_001078424.1

AUTOPHAGY 8A (APG8A); FUNCTIONS IN: APG8-specific protease activity, APG8 activating enzyme activity, Atg8 ligase activity; INVOLVED IN: autophagy; LOCATED IN: vacuolar lumen, autophagic vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Light chain 3 (LC3) (InterPro:IPR004241); BEST Arabidopsis thaliana protein match is: Ubiquitin-like superfamily protein (TAIR:AT4G04620.2); Has 1531 Blast hits to 1529 proteins in 269 species: Archae - 0; Bacteria - 0; Metazoa - 726; Fungi - 172; Plants - 305; Viruses - 3; Other Eukaryotes - 325 (source: NCBI BLink).

NP_567642.1

AUTOPHAGY 8A (APG8A); CONTAINS InterPro DOMAIN/s: Light chain 3 (LC3) (InterPro:IPR004241); BEST Arabidopsis thaliana protein match is: Ubiquitin-like superfamily protein (TAIR:AT4G04620.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

11655562..11657034

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_118319.4 → NP_567642.1 Ubiquitin-like superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_567642.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O65447, Q8LEM4, Q8S928

UniProtKB/TrEMBL

A0A178UVA3, A0A5S9XVI8

Conserved Domains (1) summary

cd16128
Location:12 → 114

Ubl_ATG8; ubiquitin-like (Ubl) domain found in Saccharomyces cerevisiae Atg8p and related proteins; sub-family of the autophagy-related 8 (ATG8) family
NM_001084955.1 → NP_001078424.1 Ubiquitin-like superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078424.1

Status: REVIEWED

UniProtKB/TrEMBL

A8MS84

Conserved Domains (1) summary

cd01611
Location:21 → 132

GABARAP; Ubiquitin domain of GABA-receptor-associated protein