RPS4 Disease resistance protein (TIR-NBS-LRR class) family [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RPS4
Official Full Name: Disease resistance protein (TIR-NBS-LRR class) family
Primary source: TAIR:AT5G45250
Locus tag: AT5G45250
See related: Araport:AT5G45250
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: DISEASE RESISTANCE PROTEIN RPS4; K9E15.1; K9E15_1; RESISTANT TO P. SYRINGAE 4
Summary: RPS4 belongs to the Toll/interleukin-1 receptor (TIR)-nucleotide binding site (NBS)-Leu-rich repeat (LRR) class of disease resistance (R) genes. Confers specific resistance to Pseudomonas syringae pv. tomato carrying the avirulence gene AvrRPS4. Produces alternative transcripts with truncated open reading frames.
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Genomic context

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See RPS4 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 5

Sequence:: Chromosome: 5; NC_003076.8 (18321764..18326175, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:: NC_003076.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RPS4 domains responsible for response to bacterial effectors from plant pathogens.
Title: Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors.
Although quantitative disease resistance (QDR) to Xanthomonas campestris is conferred by At5g22540 encoding for a protein of unknown function, QDR to XccCFBP6943 involves the well-known immune receptor pair RRS1/RPS4.
Title: Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function.
The RRS1-leucine zipper motif is crucial for the regulation of the RPS4/RRS1 complex.
Title: Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1.
Here, we report that dual R proteins, RRS1 and RPS4, from A. thaliana ecotype Wassilewskija confer resistance to bacterial wilt in transgenic Brassica crops.
Title: Arabidopsis dual resistance proteins, both RPS4 and RRS1, are required for resistance to bacterial wilt in transgenic Brassica crops.
The Arabidopsis RRS1-R nucleotide-binding, leucine-rich repeat (NB-LRR) protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense.
Title: A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors.
We show that many sushi(slh1) mutants carry mutations in RPS4, suggesting that RPS4 acts downstream or in a complex with RRS1. Interestingly, several mutations were identified in a domain C-terminal to the RPS4 LRR domain
Title: The nuclear immune receptor RPS4 is required for RRS1SLH1-dependent constitutive defense activation in Arabidopsis thaliana.
findings show RPS4 and RRS1 physically associate; TIR domain heterodimerization is required to form a functional RRS1/RPS4 effector recognition complex; RPS4 and RRS1 function as a receptor complex in which the 2 components play distinct roles in recognition and signaling
Title: Structural basis for assembly and function of a heterodimeric plant immune receptor.
A pair of Arabidopsis nucleotide binding and leucine rich repeat-type R genes, RPS4 and RRS1, properly function in two other Brassicaceae, Brassica rapa and Brassica napus, but also in two Solanaceae, Nicotiana benthamiana and tomato.
Title: Interfamily transfer of dual NB-LRR genes confers resistance to multiple pathogens.
Abscisic acid deficiency promotes the activity and nuclear localization of RPS4 at higher temperatures.
Title: Abscisic acid deficiency antagonizes high-temperature inhibition of disease resistance through enhancing nuclear accumulation of resistance proteins SNC1 and RPS4 in Arabidopsis.
study shows EDS1 forms complexes with the TIR-NB-LRR resistance proteins RPS4 and RPS6 and the negative immune regulator SRFR1 at a cytoplasmic membrane
Title: Pathogen effectors target Arabidopsis EDS1 and alter its interactions with immune regulators.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_123893.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ADP binding	IEA Inferred from Electronic Annotation more info
enables NAD+ nucleotidase, cyclic ADP-ribose generating	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within defense response	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within innate immune response-activating signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within plant-type hypersensitive response	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in endomembrane system	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Disease resistance protein (TIR-NBS-LRR class) family

NP_199338.1

RESISTANT TO P. SYRINGAE 4 (RPS4); CONTAINS InterPro DOMAIN/s: NB-ARC (InterPro:IPR002182), Toll-Interleukin receptor (InterPro:IPR000157), Disease resistance protein (InterPro:IPR000767); BEST Arabidopsis thaliana protein match is: Disease resistance protein (TIR-NBS-LRR class) family (TAIR:AT5G45060.1); Has 21522 Blast hits to 16345 proteins in 586 species: Archae - 12; Bacteria - 1045; Metazoa - 1640; Fungi - 92; Plants - 18158; Viruses - 0; Other Eukaryotes - 575 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

18321764..18326175 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_123893.2 → NP_199338.1 Disease resistance protein (TIR-NBS-LRR class) family [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_199338.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C4B7M7, Q9SCX7, Q9XGM3

Conserved Domains (6) summary

PLN03210
Location:16 → 1108

PLN03210; Resistant to P. syringae 6; Provisional

sd00033
Location:685 → 708

LRR_RI; leucine-rich repeat [structural motif]

sd00034
Location:662 → 684

LRR_AMN1; leucine-rich repeat [structural motif]

pfam01582
Location:16 → 177

TIR; TIR domain

pfam07725
Location:638 → 657

LRR_3; Leucine Rich Repeat

pfam13191
Location:208 → 343

AAA_16; AAA ATPase domain