U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    EIR1 Auxin efflux carrier family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 835813, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    EIR1
    Official Full Name
    Auxin efflux carrier family protein
    Primary source
    TAIR:AT5G57090
    Locus tag
    AT5G57090
    See related
    Araport:AT5G57090
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AGR; AGR1; AGRAVITROPIC ROOT; AGRAVITROPIC ROOT 1; ARABIDOPSIS THALIANA PIN-FORMED 2; ATPIN2; AUXIN TRANSPORT PROTEIN EIR1; ETHYLENE INSENSITIVE ROOT 1; MM31; MUL3.3; MUL3_3; PIN-FORMED 2; PIN2; POLAR-AUXIN-TRANSPORT EFFLUX COMPONENT AGRAVITROPIC 1; WAV6; WAVY ROOTS 6
    Summary
    Encodes an auxin efflux carrier that is similar to bacterial membrane transporters. Root-specific role in the transport of auxin. Acts downstream of CTR1 and ethylene biosynthesis, in the same pathway as EIN2 and AUX1, and independent from EIN3 and EIN5/AIN1 pathway. In the root, the protein localizes apically in epidermal and lateral root cap cells and predominantly basally in cortical cells. Functions may be regulated by phosphorylation status. EIR1 expression is induced by brassinolide treatment in the brassinosteroid-insensitive br1 mutant. Gravistimulation resulted in asymmetric PIN2 distribution, with more protein degraded at the upper side of the gravistimulated root. Protein turnover is affected by the proteasome and by endosomal cycling. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. Membrane sterol composition is essential for the acquisition of PIN2 polarity.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See EIR1 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    9
    Sequence:
    Chromosome: 5; NC_003076.8 (23100516..23105228)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene hydroxyproline-rich glycoprotein family protein Neighboring gene uncharacterized protein Neighboring gene Nucleotide/sugar transporter family protein Neighboring gene autoinhibited Ca2+ -ATPase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. PIN2 turnover in Arabidopsis root epidermal cells explored by the photoconvertible protein Dendra2. Jásik J, et al. PLoS One, 2013. PMID 23637828, Free PMC Article
    2. Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution. Retzer K, et al. Int J Mol Sci, 2017 Oct 29. PMID 29109378, Free PMC Article
    3. Effects of Auxins on PIN-FORMED2 (PIN2) Dynamics Are Not Mediated by Inhibiting PIN2 Endocytosis. Jásik J, et al. Plant Physiol, 2016 Oct. PMID 27506239, Free PMC Article
    4. The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana. Pan J, et al. Plant Cell, 2009 Feb. PMID 19218398, Free PMC Article
    5. Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth. Löfke C, et al. J Exp Bot, 2015 Aug. PMID 26041320, Free PMC Article

    See all (77) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis.
      Title: Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis.
    2. Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.
      Title: Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.
    3. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.
      Title: Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.
    4. The Arabidopsis STE20/Hippo kinase SIK1 regulates polarity independently of PIN proteins.
      Title: The Arabidopsis STE20/Hippo kinase SIK1 regulates polarity independently of PIN proteins.
    5. The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.
      Title: The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.
    6. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
      Title: Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
    7. Glucose-TOR signaling regulates PIN2 stability to orchestrate auxin gradient and cell expansion in Arabidopsis root.
      Title: Glucose-TOR signaling regulates PIN2 stability to orchestrate auxin gradient and cell expansion in Arabidopsis root.
    8. Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.
      Title: Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.
    9. Nickel Toxicity Targets Cell Wall-Related Processes and PIN2-Mediated Auxin Transport to Inhibit Root Elongation and Gravitropic Responses in Arabidopsis.
      Title: Nickel Toxicity Targets Cell Wall-Related Processes and PIN2-Mediated Auxin Transport to Inhibit Root Elongation and Gravitropic Responses in Arabidopsis.
    10. The polar distribution of PIN2 (also known as EIR 1) is influenced by PLC2 in the course of auxin-mediated root development in Arabidopsis
      Title: Phosphatidylinositol-specific phospholipase C2 functions in auxin-modulated root development.
    Submit: New GeneRIF Correction See all GeneRIFs (62)

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_001345238.1
    NM_125091.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables auxin efflux transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within auxin export across the plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within auxin polar transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive gravitropism IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to auxin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to ethylene IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to glucose IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to hypoxia IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in basal part of cell IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in basal plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in lytic vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    Auxin efflux carrier family protein
    NP_568848.1
    • ETHYLENE INSENSITIVE ROOT 1 (EIR1); CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT2G01420.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      23100516..23105228
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_125091.4 → NP_568848.1  Auxin efflux carrier family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_568848.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O82810, Q9LU77, Q9SYT2
      UniProtKB/TrEMBL
      A0A178UL78, A0A384LBE9, A0A5S9YES6, Q67YW0
      Conserved Domains (2) summary
      pfam03547
      Location:9 → 190
      Mem_trans; Membrane transport protein
      cl22894
      Location:486 → 646
      Mem_trans; Membrane transport protein

    2. NM_001345238.1 → NP_001331451.1  Auxin efflux carrier family protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1P8BDV9, A0A5S9YES6
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LU77.2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools