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    NFS1 nitrogen fixation S (NIFS)-like 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 836701, updated on 18-Sep-2024

    Summary

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    Official Symbol
    NFS1
    Official Full Name
    nitrogen fixation S (NIFS)-like 1
    Primary source
    TAIR:AT5G65720
    Locus tag
    AT5G65720
    See related
    Araport:AT5G65720
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ARABIDOPSIS THALIANA NITROGEN FIXATION S (NIFS)-LIKE 1; ATNFS1; ATNIFS1; CYSTEINE DESULFURASE; MPA24.7; MPA24_7; NIFS1; nitrogen fixation S (NIFS)-like 1; NITROGEN FIXATION S HOMOLOG 1
    Summary
    Encodes a cysteine desulfurase whose activity is dependent on AtSufE activation. It requires pyridoxal phosphate (PLP) for proper folding. Its catalytic efficiency is increase three-fold in the presence of AtFH (frataxin).
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    Genomic context

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    See NFS1 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    2
    Sequence:
    Chromosome: 5; NC_003076.8 (26296025..26298091)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene HAESA-like 2 Neighboring gene xyloglucan endotransglucosylase/hydrolase 6 Neighboring gene zinc ion binding protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural and functional studies of the mitochondrial cysteine desulfurase from Arabidopsis thaliana. Turowski VR, et al. Mol Plant, 2012 Sep. PMID 22511606
    2. Functional analysis of Arabidopsis genes involved in mitochondrial iron-sulfur cluster assembly. Frazzon AP, et al. Plant Mol Biol, 2007 Jun. PMID 17417719
    3. AtSufE is an essential activator of plastidic and mitochondrial desulfurases in Arabidopsis. Xu XM, et al. EMBO J, 2006 Feb 22. PMID 16437155, Free PMC Article
    4. Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix. Ito J, et al. J Proteome Res, 2006 Dec. PMID 17137349
    5. Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function. Tan YF, et al. Plant Physiol, 2010 Feb. PMID 20018591, Free PMC Article

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. AtFH binds and modulates Nfs1 kinetics in mitochondria.
      Title: Structural and functional studies of the mitochondrial cysteine desulfurase from Arabidopsis thaliana.
    2. NFS1 has a role in mediating the assembly of iron-sulfur clusters in mitochondria.
      Title: Functional analysis of Arabidopsis genes involved in mitochondrial iron-sulfur cluster assembly.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001085333.1
    NM_001345717.1
    NM_125969.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding HDA PubMed 
    enables cysteine desulfurase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine desulfurase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding HDA PubMed 
    Process Evidence Code Pubs
    involved_in [2Fe-2S] cluster assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within iron-sulfur cluster assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in mitochondrion HDA PubMed 
    located_in plastid HDA PubMed 

    General protein information

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    Preferred Names
    nitrogen fixation S (NIFS)-like 1
    NP_001078802.1
    • nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 19057 Blast hits to 19051 proteins in 2851 species: Archae - 382; Bacteria - 13739; Metazoa - 315; Fungi - 216; Plants - 123; Viruses - 11; Other Eukaryotes - 4271 (source: NCBI BLink).
    NP_001318886.1
    • nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      26296025..26298091
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001345717.1 → NP_001318886.1  nitrogen fixation S (NIFS)-like 1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O49543
      UniProtKB/TrEMBL
      A0A178UTE9, A0A5S9YHC5, A0A7G2FMY6
      Conserved Domains (1) summary
      cl18945
      Location:54 → 453
      AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...

    2. NM_125969.3 → NP_201373.1  nitrogen fixation S (NIFS)-like 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_201373.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O49543
      UniProtKB/TrEMBL
      A0A178UTE9, A0A5S9YHC5, A0A7G2FMY6
      Conserved Domains (1) summary
      cl18945
      Location:54 → 453
      AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...

    3. NM_001085333.1 → NP_001078802.1  nitrogen fixation S (NIFS)-like 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001078802.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2FMY6, A8MSF1
      Conserved Domains (1) summary
      cl18945
      Location:1 → 325
      AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O49543.1

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