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    KPNB1 karyopherin subunit beta 1 [ Homo sapiens (human) ]

    Gene ID: 3837, updated on 10-Dec-2024

    Summary

    Official Symbol
    KPNB1provided by HGNC
    Official Full Name
    karyopherin subunit beta 1provided by HGNC
    Primary source
    HGNC:HGNC:6400
    See related
    Ensembl:ENSG00000108424 MIM:602738; AllianceGenome:HGNC:6400
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    IMB1; IPO1; IPOB; Impnb; NTF97
    Summary
    Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
    Expression
    Ubiquitous expression in testis (RPKM 74.8), appendix (RPKM 47.4) and 25 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See KPNB1 in Genome Data Viewer
    Location:
    17q21.32
    Exon count:
    23
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (47649919..47685505)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (48511703..48547295)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (45727285..45762871)

    Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene mitochondrial ribosomal protein L45 pseudogene 2 Neighboring gene MPRA-validated peak2874 silencer Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12312 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45569915-45570430 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr17:45579384-45580583 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8627 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45598943-45599444 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8628 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8629 Neighboring gene ReSE screen-validated silencer GRCh37_chr17:45614024-45614225 Neighboring gene aminopeptidase puromycin sensitive Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45643631-45644132 Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45644133-45644632 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45693347-45693848 Neighboring gene KPNB1 divergent transcript Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45725440-45726285 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8632 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8630 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8631 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8633 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8634 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8635 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12313 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12314 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45771963-45772642 Neighboring gene H3K27ac hESC enhancer GRCh37_chr17:45771282-45771962 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:45772643-45773323 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12315 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8638 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45791473-45791974 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:45791975-45792474 Neighboring gene TBK1 binding protein 1 Neighboring gene TBX21 promoter region Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8640 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12316 Neighboring gene T-box transcription factor 21

    Genomic regions, transcripts, and products

    Expression

    • Project title: Tissue-specific circular RNA induction during human fetal development
    • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
    • BioProject: PRJNA270632
    • Publication: PMID 26076956
    • Analysis date: Mon Apr 2 22:54:59 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    BioGRID CRISPR Screen Phenotypes (864 hits/1261 screens)

    EBI GWAS Catalog

    Description
    Genetic Loci Associated with Circulating Levels of Very Long-Chain Saturated Fatty Acids.
    EBI GWAS Catalog
    Genome-wide meta-analysis identifies novel multiple sclerosis susceptibility loci.
    EBI GWAS Catalog

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of karyopherin (importin) beta 1 (KPNB1) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify karyopherin/importin beta 1 (KPNB1; nuclear factor p97), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify karyopherin/importin beta 1 (KPNB1; nuclear factor p97), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify karyopherin/importin beta 1 (KPNB1; nuclear factor p97), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Rev rev HIV-1 Rev interacting protein, karyopherin (importin) beta 1, is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells PubMed
    rev HIV-1 Rev nuclear import mediated by importin beta is selectively blocked by competitive excess of HIC in a cell-specific fashion PubMed
    rev importin beta can inhibit the interaction of HIV-1 Rev with HIV-1 RRE RNA PubMed
    rev importin beta specifically binds to the Rev nuclear localization signal (NLS, amino acids 35-50) and mediates the nuclear import of Rev PubMed
    Tat tat HIV-1 and the viral protein Tat modulate the expression of karyopherin (importin) beta 1 (KPNB1) in immature dendritic cells and monocyte-derived macrophages PubMed
    tat HIV-1 Tat downregulates karyopherin (importin) beta 1 in HEK 293T cells PubMed
    tat HIV-1 Tat peptide (amino-acids 47-57) binds to importin alpha and beta receptors PubMed
    tat Importin beta binds to the HIV-1 Tat protein nuclear localization signal (NLS; amino acids 49-57) and mediates the nuclear import of Tat through a novel import pathway PubMed
    tat The binding of HIV-1 Tat with importin beta is inhibited by RanGTP PubMed
    Vpr vpr HIV-1 Vpr causes increased levels of CyclinB1, Plk1, and Cdk1 in a complex with the nuclear transport and spindle assembly protein, importin beta PubMed
    vpr HIV-1 Vpr co-localizes with karyopherin beta and regulates karyopherin beta-mediated docking of the HIV-1 preintegration complex at the nuclear envelope, however multiple reports indicate the two proteins do not directly interact PubMed
    integrase gag-pol Ivermectin, an inhibitor of importin alpha/beta1, inhibits HIV-1 infection by blocking the HIV-1 Integrase interaction with importin alpha/beta1 for viral protein nuclear import PubMed
    gag-pol Some reports indicate a possible role for the interactions between karyopherin alpha and beta with HIV-1 integrase in the nuclear import of HIV-1 preintegration complexes (PIC), while other reports indicate integrase is not involved in PIC nuclear import PubMed
    gag-pol Karyopherin beta binds to complexes between HIV-1 integrase (integrase/GST fusion protein) and karyopherin alpha, but not to integrase alone PubMed
    gag-pol Karyopherin alpha and beta are reported to interact with HIV-1 integrase (IN) to facilitate nuclear import of IN, however a conflicting report indicates nuclear accumulation of IN does not involve karyopherin alpha, beta 1, or beta 2 mediated pathways PubMed
    matrix gag Karyopherin beta mediates the nuclear import of HIV-1 preintegration complexes through karyopherin alpha, which binds directly to nuclear localization signals in HIV-1 Matrix PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    General gene information

    Clone Names

    • MGC2155, MGC2156, MGC2157

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables Hsp90 protein binding  
    enables RNA binding PubMed 
    enables enzyme binding PubMed 
    enables importin-alpha family protein binding  
    enables nuclear import signal receptor activity  
    enables nuclear import signal receptor activity PubMed 
    enables nuclear import signal receptor activity  
    enables nuclear localization sequence binding  
    enables protein binding PubMed 
    enables protein domain specific binding PubMed 
    enables small GTPase binding  
    enables zinc ion binding PubMed 
    Process Evidence Code Pubs
    involved_in NLS-bearing protein import into nucleus PubMed 
    involved_in NLS-bearing protein import into nucleus  
    involved_in RNA import into nucleus PubMed 
    involved_in astral microtubule organization PubMed 
    involved_in establishment of mitotic spindle localization PubMed 
    involved_in mitotic chromosome movement towards spindle pole PubMed 
    involved_in mitotic metaphase chromosome alignment PubMed 
    involved_in mitotic spindle assembly PubMed 
    involved_in positive regulation of cholesterol biosynthetic process  
    involved_in protein import into nucleus  
    acts_upstream_of_or_within protein import into nucleus PubMed 
    involved_in protein import into nucleus PubMed 
    involved_in protein import into nucleus PubMed 
    involved_in protein import into nucleus  
    involved_in ribosomal protein import into nucleus PubMed 
    Items 1 - 20 of 22
    Component Evidence Code Pubs
    part_of NLS-dependent protein nuclear import complex PubMed 
    part_of NLS-dependent protein nuclear import complex  
    is_active_in cytoplasm  
    is_active_in cytoplasm PubMed 
    is_active_in cytoplasm  
    located_in cytoplasmic stress granule  
    located_in cytosol  
    located_in cytosol PubMed 
    located_in cytosol  
    located_in endoplasmic reticulum tubular network PubMed 
    located_in extracellular exosome PubMed 
    located_in extracellular region  
    located_in ficolin-1-rich granule lumen  
    located_in membrane PubMed 
    located_in nuclear envelope PubMed 
    located_in nuclear membrane  
    part_of nuclear pore PubMed 
    located_in nucleoplasm PubMed 
    located_in nucleoplasm PubMed 
    located_in nucleoplasm  
    is_active_in nucleus  
    located_in specific granule lumen  
    Items 1 - 20 of 22

    General protein information

    Preferred Names
    importin subunit beta-1
    Names
    PTAC97
    importin 1
    importin 90
    importin beta-1 subunit
    karyopherin (importin) beta 1
    nuclear factor p97
    pore targeting complex 97 kDa subunit

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001276453.2 → NP_001263382.1  importin subunit beta-1 isoform 2

      See identical proteins and their annotated locations for NP_001263382.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) has an alternate exon in place of the first two exons compared to variant 1, which causes translation to begin at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.
      Source sequence(s)
      AC015674, AC025682, KF570270
      Consensus CDS
      CCDS62228.1
      UniProtKB/TrEMBL
      B7Z752
      Related
      ENSP00000438964.1, ENST00000540627.5
      Conserved Domains (2) summary
      COG5215
      Location:2 → 726
      KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]
      sd00044
      Location:178 → 209
      HEAT; HEAT repeat [structural motif]
    2. NM_002265.6 → NP_002256.2  importin subunit beta-1 isoform 1

      See identical proteins and their annotated locations for NP_002256.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC015674, AC025682, KF570270
      Consensus CDS
      CCDS11513.1
      UniProtKB/Swiss-Prot
      B7ZAV6, D3DTT3, Q14637, Q14974, Q53XN2, Q96J27
      UniProtKB/TrEMBL
      B2RBR9
      Related
      ENSP00000290158.3, ENST00000290158.9
      Conserved Domains (2) summary
      COG5215
      Location:2 → 871
      KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]
      sd00044
      Location:323 → 354
      HEAT; HEAT repeat [structural motif]

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

      Range
      47649919..47685505
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060941.1 Alternate T2T-CHM13v2.0

      Range
      48511703..48547295
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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