SKP1 S phase kinase-associated protein 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: SKP1
Official Full Name: S phase kinase-associated protein 1
Primary source: TAIR:AT1G75950
Locus tag: AT1G75950
See related: Araport:AT1G75950
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS SKP1 HOMOLOGUE 1; ASK1; ATSKP1; S phase kinase-associated protein 1; SKP1A; T4O12.17; T4O12_17; UFO INTERACTING PROTEIN 1; UIP1
Summary: SKP1 is core component of the SCF family of E3 ubiquitin ligases and serves to tether the rest of the complex to an F-box protein, which provides specificity in binding to ubiquitin ligase substrate proteins. Predominately expressed from leptotene to pachytene. Negatively regulates recombination. Interacts with P0, a silencing suppressor protein encoded by poleroviruses by means of a conserved minimal F-box motif.
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Genomic context

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See SKP1 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 2

Sequence:: Chromosome: 1; NC_003070.9 (28516519..28517896)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The de-ubiquitinase UBQUITIN SPECIFIC PROTEASE 15 (UBP15) interacts with the SCF E3 complex adaptor ARABIDOPSIS SKP1 HOMOLOGUE 1 (ASK1) to regulate petal size and fertility in Arabidopsis thaliana.
Title: The de-ubiquitinase UBQUITIN SPECIFIC PROTEASE 15 (UBP15) interacts with the SCF E3 complex adaptor ARABIDOPSIS SKP1 HOMOLOGUE 1 (ASK1) to regulate petal size and fertility in Arabidopsis thaliana.
In vitro and in vivo protein-protein interaction assays show that NO enhances ASK1 binding to CUL1 and TIR1/AFB2, required for SCF(TIR1/AFB2) assembly.
Title: Regulation of SCF(TIR1/AFBs) E3 ligase assembly by S-nitrosylation of Arabidopsis SKP1-like1 impacts on auxin signaling.
Arabidopsis SKP-like proteins (ASKs) can significantly improve soluble expression of F-box proteins and maintain their bioactivity.
Title: Efficient ASK-assisted system for expression and purification of plant F-box proteins.
How ASK1 might regulate protein stability and further downstream gene expression
Title: Proteomics and transcriptomics analyses of Arabidopsis floral buds uncover important functions of ARABIDOPSIS SKP1-LIKE1.
ABA induced the phosphorylation of three basic helix-loop-helix (bHLH) transcription factors, called AKSs (ABA-responsive kinase substrates; AKS1, AKS2, and AKS3), in Arabidopsis guard cells
Title: bHLH transcription factors that facilitate K⁺ uptake during stomatal opening are repressed by abscisic acid through phosphorylation.
ASK1 and ASK2, are required for Agrobacterium-mediated plant transformation.
Title: Several components of SKP1/Cullin/F-box E3 ubiquitin ligase complex and associated factors play a role in Agrobacterium-mediated plant transformation.
ASK1 or ASK2 overexpression could rescue or partially rescue the Abscisic acid (ABA)insensitivity of abi5-1 mutants, respectively.
Title: SKP1 is involved in abscisic acid signalling to regulate seed germination, stomatal opening and root growth in Arabidopsis thaliana.
study represents a thorough investigation showing that retroposition can play an important role in the evolution of a plant gene family whose members do not encode mobile elements
Title: Patterns of gene duplication in the plant SKP1 gene family in angiosperms: evidence for multiple mechanisms of rapid gene birth.
ASK1 is predominately expressed from leptotene to pachytene, and negatively regulates recombination in Arabidopsis.
Title: The ARABIDOPSIS SKP1-LIKE1 (ASK1) protein acts predominately from leptotene to pachytene and represses homologous recombination in male meiosis.
Through the ASK1-mediated proteolysis pathway, ASK1 proteins showed pleiotropic functions including photomorphogenesis, circadian oscillation, post-translation process, stress-responses and cell expansion or elongation.
Title: Proteomic identification of potential target proteins regulated by an ASK1-mediated proteolysis pathway.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_106245.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within negative regulation of DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	NAS Non-traceable Author Statement more info
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytoplasm	NAS Non-traceable Author Statement more info
located_in cytosol	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info
located_in phragmoplast	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin ligase complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: S phase kinase-associated protein 1

NP_565123.1

S phase kinase-associated protein 1 (SKP1); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: negative regulation of DNA recombination, response to cadmium ion, mitosis, male meiosis, ubiquitin-dependent protein catabolic process; LOCATED IN: in 7 components; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: E3 ubiquitin ligase, SCF complex, Skp subunit (InterPro:IPR016897), SKP1 component, dimerisation (InterPro:IPR016072), SKP1 component (InterPro:IPR001232), BTB/POZ fold (InterPro:IPR011333), SKP1 component, POZ (InterPro:IPR016073); BEST Arabidopsis thaliana protein match is: E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein (TAIR:AT5G42190.1); Has 1455 Blast hits to 1451 proteins in 268 species: Archae - 0; Bacteria - 0; Metazoa - 552; Fungi - 179; Plants - 538; Viruses - 11; Other Eukaryotes - 175 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

28516519..28517896

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_106245.5 → NP_565123.1 S phase kinase-associated protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565123.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P92992, Q39255

UniProtKB/TrEMBL

A0A178WD95, A0A5S9WUU4

Conserved Domains (2) summary

cd18322
Location:5 → 125

BTB_POZ_SKP1; BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in S-phase kinase-associated protein 1 (SKP1) and similar proteins

pfam01466
Location:111 → 158

Skp1; Skp1 family, dimerization domain