Tnik TRAF2 and NCK interacting kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tnikprovided by MGI
Official Full Name: TRAF2 and NCK interacting kinaseprovided by MGI
Primary source: MGI:MGI:1916264
See related: Ensembl:ENSMUSG00000027692 AllianceGenome:MGI:1916264
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 1500031A17Rik; 4831440I19Rik; C530008O15Rik; C630040K21Rik
Summary: Predicted to enable protein serine/threonine kinase activity. Involved in regulation of neurotransmitter receptor localization to postsynaptic specialization membrane. Is active in glutamatergic synapse. Human ortholog(s) of this gene implicated in autosomal recessive intellectual developmental disorder 54. Orthologous to human TNIK (TRAF2 and NCK interacting kinase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in frontal lobe adult (RPKM 7.4), cortex adult (RPKM 5.7) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 A3; 3 11.92 cM

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (28317362..28724734)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (28261901..28670585)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TNIK is a conserved regulator of glucose and lipid metabolism in obesity.
Title: TNIK is a conserved regulator of glucose and lipid metabolism in obesity.
Traf2 and NCK Interacting Kinase Is a Critical Regulator of Procollagen I Trafficking and Hepatic Fibrogenesis in Mice.
Title: Traf2 and NCK Interacting Kinase Is a Critical Regulator of Procollagen I Trafficking and Hepatic Fibrogenesis in Mice.
Phosphorylation-dependent control of Activity-regulated cytoskeleton-associated protein (Arc) protein by TNIK.
Title: Phosphorylation-dependent control of Activity-regulated cytoskeleton-associated protein (Arc) protein by TNIK.
TNIK is an important regulator of effector and memory T cell differentiation and induces a population of stem cell-like memory T cells.
Title: TNIK signaling imprints CD8(+) T cell memory formation early after priming.
TNIK is required for the tumour-initiating function of colorectal cancer stem cells. Its inhibition is a promising therapeutic approach.
Title: TNIK inhibition abrogates colorectal cancer stemness.
This study demonstrate here that TNIK is expressed in neurons throughout the adult mouse brain.
Title: Organization of TNIK in dendritic spines.
TNiK is required for dentate gyrus neurogenesis & for pattern separation in a 2-choice spatial discrimination task & for glutamate-dependent object-location paired associate learning.
Title: TNiK is required for postsynaptic and nuclear signaling pathways and cognitive function.
TNIK maintains levels of glutamate receptors and other key postsynaptic density (PSD) proteins at the synapse, with loss of TNIK protein or inhibition of TNIK activity causing rapid degradation of PSD constituents.
Title: The psychiatric disease risk factors DISC1 and TNIK interact to regulate synapse composition and function.
siRNA depletion of TNIK followed by expression array analysis showed that TNIK is an essential, specific activator of Wnt transcriptional programme.
Title: The kinase TNIK is an essential activator of Wnt target genes.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (2)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH124975 (e-PCR)
- UniSTS:161797

D3Mit55 (e-PCR), detects polymorphism
- UniSTS:129823

D3Mit325 (e-PCR), detects polymorphism
- UniSTS:142391

RH124560 (e-PCR)
- UniSTS:161382

AI451411 (e-PCR)
- UniSTS:165243

RH125646 (e-PCR)
- UniSTS:162434

AW532604 (e-PCR)
- UniSTS:260568

BQ207408 (e-PCR)
- UniSTS:261611

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in microvillus assembly	ISO Inferred from Sequence Orthology more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in neuron projection morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of JNK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of JNK cascade	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of dendrite morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of neurotransmitter receptor localization to postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of neurotransmitter receptor localization to postsynaptic specialization membrane	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in postsynaptic density, intracellular component	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
located_in recycling endosome	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: traf2 and NCK-interacting protein kinase

NP_001156479.1

EC 2.7.11.1

NP_001156480.1

EC 2.7.11.1

NP_001156481.1

EC 2.7.11.1

NP_081186.1

EC 2.7.11.1

XP_006535580.1

EC 2.7.11.1

XP_006535581.1

EC 2.7.11.1

XP_006535582.1

EC 2.7.11.1

XP_006535583.1

EC 2.7.11.1

XP_006535584.1

EC 2.7.11.1

XP_011248006.1

EC 2.7.11.1

XP_011248007.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163007.1 → NP_001156479.1 traf2 and NCK-interacting protein kinase isoform 2

See identical proteins and their annotated locations for NP_001156479.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an internal, in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) missing an 8 aa protein segment compared to isoform 1.

Source sequence(s)

BC050868, CK620825

Consensus CDS

CCDS50878.1

UniProtKB/TrEMBL

B2RXX2, B9EKN8

Related

ENSMUSP00000124876.3, ENSMUST00000159680.9

Conserved Domains (2) summary

smart00036
Location:1034 → 1332

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
NM_001163008.1 → NP_001156480.1 traf2 and NCK-interacting protein kinase isoform 3

See identical proteins and their annotated locations for NP_001156480.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two internal, in-frame coding exons compared to variant 1, resulting in a shorter isoform (3) missing two protein segments compared to isoform 1.

Source sequence(s)

BC050868

Consensus CDS

CCDS50879.1

UniProtKB/Swiss-Prot

P83510

UniProtKB/TrEMBL

B2RXX2

Related

ENSMUSP00000124681.3, ENSMUST00000159236.9

Conserved Domains (3) summary

smart00036
Location:1005 → 1303

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

smart00220
Location:25 → 289

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
NM_001163009.1 → NP_001156481.1 traf2 and NCK-interacting protein kinase isoform 4

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks an internal, in-frame coding exon, and differs at the 3' end compared to variant 1, resulting in a longer isoform (4) with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC121268, AI115107, BC050868, CF531435, CK620825

UniProtKB/TrEMBL

B2RY17

Related

ENSMUST00000162037.9

Conserved Domains (3) summary

smart00036
Location:1034 → 1326

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

smart00220
Location:25 → 289

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
NM_026910.1 → NP_081186.1 traf2 and NCK-interacting protein kinase isoform 1

See identical proteins and their annotated locations for NP_081186.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform 1.

Source sequence(s)

BC050868, CD348964, CK620825

Consensus CDS

CCDS50877.1

UniProtKB/TrEMBL

B2RQ80, B2RXX2

Related

ENSMUSP00000125081.3, ENSMUST00000160307.9

Conserved Domains (3) summary

smart00036
Location:1042 → 1340

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

smart00220
Location:25 → 289

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

28317362..28724734

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006535519.4 → XP_006535582.1 traf2 and NCK-interacting protein kinase isoform X3

UniProtKB/TrEMBL

B2RXX2

Conserved Domains (2) summary

smart00036
Location:1045 → 1343

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
XM_006535517.4 → XP_006535580.1 traf2 and NCK-interacting protein kinase isoform X1

UniProtKB/TrEMBL

B2RY17

Conserved Domains (2) summary

smart00036
Location:1045 → 1337

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
XM_006535518.4 → XP_006535581.1 traf2 and NCK-interacting protein kinase isoform X2

UniProtKB/TrEMBL

B2RY17

Conserved Domains (2) summary

smart00036
Location:1042 → 1334

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
XM_006535521.4 → XP_006535584.1 traf2 and NCK-interacting protein kinase isoform X5

UniProtKB/TrEMBL

B2RXX2, E9PUL9

Related

ENSMUSP00000124011.2, ENSMUST00000160518.8

Conserved Domains (2) summary

smart00036
Location:1013 → 1311

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
XM_006535520.4 → XP_006535583.1 traf2 and NCK-interacting protein kinase isoform X4

UniProtKB/TrEMBL

B2RY17

Conserved Domains (2) summary

smart00036
Location:1016 → 1308

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd06637
Location:18 → 313

STKc_TNIK; Catalytic domain of the Serine/Threonine Kinase, Traf2- and Nck-Interacting Kinase
XM_011249704.4 → XP_011248006.1 traf2 and NCK-interacting protein kinase isoform X6

UniProtKB/TrEMBL

B2RY17

Conserved Domains (2) summary

smart00036
Location:990 → 1282

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cl21453
Location:1 → 258

PKc_like; Protein Kinases, catalytic domain
XM_011249705.4 → XP_011248007.1 traf2 and NCK-interacting protein kinase isoform X7

UniProtKB/TrEMBL

B2RY17

Conserved Domains (2) summary

smart00036
Location:961 → 1253

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cl21453
Location:1 → 258

PKc_like; Protein Kinases, catalytic domain