E2f4 E2F transcription factor 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: E2f4provided by MGI
Official Full Name: E2F transcription factor 4provided by MGI
Primary source: MGI:MGI:103012
See related: Ensembl:ENSMUSG00000014859 AllianceGenome:MGI:103012
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2010111M04Rik
Summary: Enables DNA-binding transcription factor activity; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Acts upstream of or within several processes, including cell volume homeostasis; cilium assembly; and positive regulation of transcription by RNA polymerase II. Located in chromatin and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study otitis media. Human ortholog(s) of this gene implicated in Lynch syndrome. Orthologous to human E2F4 (E2F transcription factor 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in liver E14.5 (RPKM 188.0), liver E14 (RPKM 147.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 D3; 8 53.04 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (106024295..106032002)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (105297663..105305370)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis.
Title: In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis.
Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.
Title: Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.
A Mutant Variant of E2F4 Triggers Multifactorial Therapeutic Effects in 5xFAD Mice.
Title: A Mutant Variant of E2F4 Triggers Multifactorial Therapeutic Effects in 5xFAD Mice.
E2F1 and E2F4 regulated Cited2 expression in neurons after stroke-related insults. Taken together, these results indicate that the E2F-Cited2 regulatory pathway is critically involved in stroke injury.
Title: The pro-death role of Cited2 in stroke is regulated by E2F1/4 transcription factors.
E2F4 is important for the proliferation and the survival of mouse embryonic stem cells.
Title: E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family.
E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these organizing centres and that, without cytoplasmic E2f4, deuterosomes are not assembled, halting multiciliogenesis
Title: Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
E2f4 and E2f5 display overlapping roles in controlling the normal development of the male reproductive system.
Title: E2f4 and E2f5 are essential for the development of the male reproductive system.
Following stress exposure, E2F4-p130 complexes are lost rapidly along with the presence of E2F4 at E2F-containing B-Myb promoter sites.
Title: Regulation of ischemic neuronal death by E2F4-p130 protein complexes.
E2F4 is part of a transcriptional repressor complex comprising p130 and HDAC1 in cardiac myocytes. Relief from E2F4-dependent gene silencing induces the expression of pro-apoptotic genes.
Title: Anti-apoptotic function of the E2F transcription factor 4 (E2F4)/p130, a member of retinoblastoma gene family in cardiac myocytes.
In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component.
Title: Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (32)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI427446 (e-PCR)
- UniSTS:159800

E2f4 (e-PCR), detects polymorphism
- UniSTS:536690

E2F4_601 (e-PCR)
- UniSTS:277179

E2F4_V13 (e-PCR)
- UniSTS:277179

NoName (e-PCR)
- UniSTS:235980

E2f4 (e-PCR), detects polymorphism
- UniSTS:506870

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	TAS Traceable Author Statement more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within animal organ morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood circulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell volume homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centriole assembly	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epithelial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in motile cilium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in multi-ciliated epithelial cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within regulation of cell cycle	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within regulation of cell population proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	TAS Traceable Author Statement more info	PubMed
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription factor E2F4

Names: E2F-4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_148952.1 → NP_683754.1 transcription factor E2F4

See identical proteins and their annotated locations for NP_683754.1

Status: PROVISIONAL

Source sequence(s)

BC026649

Consensus CDS

CCDS40456.1

UniProtKB/Swiss-Prot

Q8R0K9, Q8R2X6

Related

ENSMUSP00000015003.9, ENSMUST00000015003.10

Conserved Domains (2) summary

cd14660
Location:95 → 198

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:19 → 83

E2F_TDP; E2F/DP family winged-helix DNA-binding domain