Arhgef28 Rho guanine nucleotide exchange factor 28 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Arhgef28provided by MGI
Official Full Name: Rho guanine nucleotide exchange factor 28provided by MGI
Primary source: MGI:MGI:1346016
See related: Ensembl:ENSMUSG00000021662 AllianceGenome:MGI:1346016
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RIP2; Rgnef; RhoGEF; Rhoip2; p190RhoGEF; D13Bwg1089e; 9230110L08Rik
Summary: Predicted to enable guanyl-nucleotide exchange factor activity. Acts upstream of or within central nervous system neuron axonogenesis and neurofilament cytoskeleton organization. Predicted to be located in cytoplasm and plasma membrane. Is expressed in adrenal gland; genitourinary system; oral epithelium; paravertebral ganglion; and stomach. Orthologous to human ARHGEF28 (Rho guanine nucleotide exchange factor 28). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in kidney adult (RPKM 5.2), ovary adult (RPKM 5.2) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 D1; 13 51.6 cM

Exon count:: 42

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (98035105..98343183, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (97898594..98206675, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Over-Expression of p190RhoGEF Regulates the Formation of Atherosclerotic Plaques in the Aorta of ApoE[-/-] Mice via Macrophage Polarization.
Title: Over-Expression of p190RhoGEF Regulates the Formation of Atherosclerotic Plaques in the Aorta of ApoE(-/-) Mice via Macrophage Polarization.
A new role for Rgnef in ovarian cancer to facilitate NF-kappaB-mediated gene expression protecting cells from oxidative stress.
Title: Rgnef promotes ovarian tumor progression and confers protection from oxidative stress.
observed that the expression of RGNEF is upregulated in murine spinal motor neurons following distal sciatic nerve injury
Title: Rho guanine nucleotide exchange factor (RGNEF) is a prosurvival factor under stress conditions.
Rgnef promotes integrin beta 1-stimulated focal adhesion kinase activation.
Title: A non-canonical role for Rgnef in promoting integrin-stimulated focal adhesion kinase activation.
An essential role for Rgnef in RhoA regulation downstream of integrins in control of cell migration.
Title: Rgnef (p190RhoGEF) knockout inhibits RhoA activity, focal adhesion establishment, and cell motility downstream of integrins.
changes in cytoskeletal tension mediated by Rho signaling through ROCK may play an important role in the establishment of the spatial differentials in cell growth and extracellular matrix remodeling that drive embryonic lung development
Title: Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension.
STAT3 represents an essential effector pathway of Rho GTPases in regulating multiple cellular functions including actin cytoskeleton reorganization, cell migration, gene activation, and proliferation
Title: A role of STAT3 in Rho GTPase-regulated cell migration and proliferation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D13Bwg1089e (e-PCR), detects polymorphism
- UniSTS:141867

G67059 (e-PCR)
- UniSTS:178268

AI323540 (e-PCR)
- UniSTS:180584

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within central nervous system neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neurofilament cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho guanine nucleotide exchange factor 28

Names: 190 kDa guanine nucleotide exchange factor; Rho guanine nucleotide exchange factor (GEF) 28; Rho interacting protein 2; Rho specific exchange factor; Rho-guanine nucleotide exchange factor; p190-RhoGEF; rho-interacting protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001408684.1 → NP_001395613.1 rho guanine nucleotide exchange factor 28 isoform 2

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507
NM_001408685.1 → NP_001395614.1 rho guanine nucleotide exchange factor 28 isoform 3

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507
NM_001408686.1 → NP_001395615.1 rho guanine nucleotide exchange factor 28 isoform 4

Status: VALIDATED

Source sequence(s)

AC124397, AC133507

UniProtKB/TrEMBL

A0A571BEH9

Related

ENSMUSP00000158740.2, ENSMUST00000238746.2
NM_001408687.1 → NP_001395616.1 rho guanine nucleotide exchange factor 28 isoform 5

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507
NM_012026.3 → NP_036156.2 rho guanine nucleotide exchange factor 28 isoform 1

See identical proteins and their annotated locations for NP_036156.2

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507

Consensus CDS

CCDS36758.1

UniProtKB/Swiss-Prot

A0PJC1, B2RQQ2, G5E8P2, P97433, Q69Z41, Q6DI55

Related

ENSMUSP00000105053.2, ENSMUST00000109426.3

Conserved Domains (4) summary

cd00029
Location:652 → 697

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:847 → 1039

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd14680
Location:1084 → 1184

PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00169
Location:1120 → 1185

PH; PH domain

RNA

NR_176793.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507

Related

ENSMUST00000225884.2
NR_176794.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC123056, AC124397, AC133507

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

98035105..98343183 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006517522.4 → XP_006517585.1 rho guanine nucleotide exchange factor 28 isoform X6

Conserved Domains (4) summary

PRK00286
Location:973 → 1129

xseA; exodeoxyribonuclease VII large subunit; Reviewed

cd00029
Location:275 → 320

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:470 → 662

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cl17171
Location:707 → 807

PH-like; Pleckstrin homology-like domain
XM_017315362.3 → XP_017170851.2 rho guanine nucleotide exchange factor 28 isoform X4

Conserved Domains (4) summary

PRK00286
Location:1020 → 1176

xseA; exodeoxyribonuclease VII large subunit; Reviewed

cd00160
Location:517 → 709

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd14680
Location:754 → 854

PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

cl00040
Location:316 → 375

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_006517521.4 → XP_006517584.1 rho guanine nucleotide exchange factor 28 isoform X5

UniProtKB/TrEMBL

A0A286YDI4

Conserved Domains (3) summary

cd00029
Location:666 → 711

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:861 → 1053

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd14680
Location:1098 → 1198

PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain
XM_036157834.1 → XP_036013727.1 rho guanine nucleotide exchange factor 28 isoform X7

UniProtKB/TrEMBL

A0A286YDI4

Conserved Domains (3) summary

cd00160
Location:861 → 1053

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd14680
Location:1098 → 1198

PH_p190RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

cl00040
Location:660 → 719

C1; protein kinase C conserved region 1 (C1 domain) superfamily