Dntt deoxynucleotidyltransferase, terminal [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dnttprovided by MGI
Official Full Name: deoxynucleotidyltransferase, terminalprovided by MGI
Primary source: MGI:MGI:98659
See related: Ensembl:ENSMUSG00000025014 AllianceGenome:MGI:98659
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tdt
Summary: Enables DNA nucleotidylexotransferase activity. Acts upstream of or within DNA metabolic process. Predicted to be located in cytosol; euchromatin; and nuclear lumen. Predicted to be active in nucleus. Is expressed in bone marrow; ovary; submandibular gland; testis; and thymus. Orthologous to human DNTT (DNA nucleotidylexotransferase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Restricted expression toward thymus adult (RPKM 111.5) See more
Orthologs: human all
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Genomic context

Location:: 19 C3; 19 34.32 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (41017701..41047964)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (41029275..41059525)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dntt expression reveals developmental hierarchy and lineage specification of hematopoietic progenitors.
Title: Dntt expression reveals developmental hierarchy and lineage specification of hematopoietic progenitors.
Molecular dynamics simulations and structural analysis to decipher functional impact of a twenty residue insert in the ternary complex of mus musculus TdT isoform have been reported.
Title: Molecular Dynamics Simulations and Structural Analysis to Decipher Functional Impact of a Twenty Residue Insert in the Ternary Complex of Mus musculus TdT Isoform.
TdT is essential for the generation of the predominant higher-affinity DEX-responsive J558 clone
Title: Terminal deoxynucleotidyl transferase is required for an optimal response to the polysaccharide α-1,3 dextran.
The role of TdT ensures enhanced diversity and selection of private T cell receptor repertoires and promotes optimal CD8-positive T cell immunity, both within individuals and across the species as a whole.
Title: Terminal deoxynucleotidyltransferase is required for the establishment of private virus-specific CD8+ TCR repertoires and facilitates optimal CTL responses.
TdT plays a critical role in the magnitude and breadth of anti-viral T(CD8+) responses toward individual determinants
Title: Terminal deoxynucleotidyl transferase establishes and broadens antiviral CD8+ T cell immunodominance hierarchies.
tdt may be involved in learning and memory saving.
Title: Experience-dependent expression of terminal deoxynucleotidyl transferase in mouse brain.
onset of terminal deoxynucleotidyl transferase gene activation during T cell differentiation and thymic ontogeny
Title: Substantial N diversity is generated in T cell receptor alpha genes at birth despite low levels of terminal deoxynucleotidyl transferase expression in mouse thymus.
Structure-function analysis of proposed domains and motifs for known activity of TdT reveals nonessential role for tumor suppressor BRCA1 C-terminal (BRCT) domain and essential roles for second helix-hairpin-helix motif and entire C terminus of TdT.
Title: Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.
Production of M603-id antibody is TdT dependent, while generation of M167-id antibody is TdT independent.
Title: The M603 idiotype is lost in the response to phosphocholine in terminal deoxynucleotidyl transferase-deficient mice.
The role of this locus in autoantibody production in MRL/lpr mouse strain, and subsequent development of kidney disease.
Title: Effect of genetic deficiency of terminal deoxynucleotidyl transferase on autoantibody production and renal disease in MRL/lpr mice.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BB160593 (e-PCR)
- UniSTS:186155

Dntt (e-PCR), detects polymorphism
- UniSTS:462638

Dntt (e-PCR), detects polymorphism
- UniSTS:462639

Dntt (e-PCR), detects polymorphism
- UniSTS:144590

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA nucleotidylexotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA nucleotidylexotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA nucleotidylexotransferase activity	ISO Inferred from Sequence Orthology more info
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in DNA modification	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to ATP	IEA Inferred from Electronic Annotation more info
involved_in response to ATP	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in euchromatin	IEA Inferred from Electronic Annotation more info
located_in euchromatin	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	IEA Inferred from Electronic Annotation more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: DNA nucleotidylexotransferase

Names: terminal addition enzyme; terminal deoxynucleotidyl transferase

NP_001036693.1

EC 2.7.7.31

NP_033371.2

EC 2.7.7.31

XP_036017402.1

EC 2.7.7.31

XP_036017403.1

EC 2.7.7.31

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001043228.1 → NP_001036693.1 DNA nucleotidylexotransferase isoform 2

See identical proteins and their annotated locations for NP_001036693.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AC126608, AF316015, BX639078

Consensus CDS

CCDS37984.1

UniProtKB/Swiss-Prot

P09838, Q99PD0, Q99PD1

UniProtKB/TrEMBL

Q3UZ80

Related

ENSMUSP00000107819.2, ENSMUST00000112200.3

Conserved Domains (3) summary

cd00141
Location:166 → 508

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

smart00292
Location:32 → 111

BRCT; breast cancer carboxy-terminal domain

smart00483
Location:163 → 509

POLXc; DNA polymerase X family
NM_009345.2 → NP_033371.2 DNA nucleotidylexotransferase isoform 1

See identical proteins and their annotated locations for NP_033371.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC126608, AF316014, BX639078

Consensus CDS

CCDS29807.1

UniProtKB/Swiss-Prot

P09838

Related

ENSMUSP00000062078.5, ENSMUST00000051806.12

Conserved Domains (3) summary

cd00141
Location:166 → 528

NT_POLXc; Nucleotidyltransferase (NT) domain of family X DNA Polymerases

smart00292
Location:32 → 111

BRCT; breast cancer carboxy-terminal domain

smart00483
Location:163 → 529

POLXc; DNA polymerase X family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

41017701..41047964

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161509.1 → XP_036017402.1 DNA nucleotidylexotransferase isoform X1

Conserved Domains (2) summary

smart00483
Location:163 → 528

POLXc; DNA polymerase X family

cl00038
Location:31 → 125

BRCT; C-terminal domain of the breast cancer suppressor protein (BRCA1) and related domains
XM_036161510.1 → XP_036017403.1 DNA nucleotidylexotransferase isoform X2

Conserved Domains (2) summary

smart00483
Location:163 → 508

POLXc; DNA polymerase X family

cl00038
Location:31 → 125

BRCT; C-terminal domain of the breast cancer suppressor protein (BRCA1) and related domains