Top2b topoisomerase (DNA) II beta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Top2bprovided by MGI
Official Full Name: topoisomerase (DNA) II betaprovided by MGI
Primary source: MGI:MGI:98791
See related: Ensembl:ENSMUSG00000017485 AllianceGenome:MGI:98791
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Top-2; D230016L12Rik
Summary: Enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity. Involved in B cell differentiation. Acts upstream of or within axonogenesis; forebrain development; and neuron migration. Located in nucleus. Is expressed in central nervous system; genitourinary system; and retina. Orthologous to human TOP2B (DNA topoisomerase II beta). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E11.5 (RPKM 40.7), CNS E14 (RPKM 36.8) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 A1; 14 7.08 cM

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (6038976..6104585, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (16365206..16430787)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome.
Title: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome.
Nucleophagy contributes to genome stability through degradation of type II topoisomerases A and B and nucleolar components.
Title: Nucleophagy contributes to genome stability through degradation of type II topoisomerases A and B and nucleolar components.
Calcineurin dephosphorylates topoisomerase IIbeta and regulates the formation of neuronal-activity-induced DNA breaks.
Title: Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.
Loss of TOP2B negatively affects the B-cell development.
Title: Mutations in topoisomerase IIβ result in a B cell immunodeficiency.
Top2beta controls spinal motor neurons (MN) migration and connectivity; is not required for MN generation or survival; has a selective role in columnar specification. In absence of Top2beta, phrenic MN identity is eroded, while other motor columns are partially preserved but fail to cluster to their proper position. In Top2beta-/- mice, peripheral connectivity is impaired as MNs exhibit a profound deficit in terminal b...
Title: Topoisomerase IIβ Selectively Regulates Motor Neuron Identity and Peripheral Connectivity through Hox/Pbx-Dependent Transcriptional Programs.
our data establish a combinatorial cell-autonomous and non-cell-autonomous role for Top2b in the late stage of photoreceptor differentiation and maturation
Title: Top2b is involved in the formation of outer segment and synapse during late-stage photoreceptor differentiation by controlling key genes of photoreceptor transcriptional regulatory network.
We demonstrated that Nup98-TopIIbeta and Nup98-SETBP1 negatively regulate the XPO1-mediated protein export. Our results will contribute to the understanding of the molecular mechanism by which the Nup98-fusion proteins induce tumorigenesis.
Title: Function of Nup98 subtypes and their fusion proteins, Nup98-TopIIβ and Nup98-SETBP1 in nuclear-cytoplasmic transport.
TOP2beta as a factor involved in regulating granulosa cell genomic integrity and follicle atresia.
Title: TOP2β is essential for ovarian follicles that are hypersensitive to chemotherapeutic drugs.
Results identified topoisomerase IIbeta (Top2beta) as a novel determinant for camptothecin sensitivity.
Title: Topoisomerase IIβ deficiency enhances camptothecin-induced apoptosis.
Top IIbeta might be a down-stream target of Nurr1, which might influence the processes of axon genesis in dopaminergic neurons via the regulation of TOP IIbeta expression.
Title: Nurr1 regulates Top IIβ and functions in axon genesis of mesencephalic dopaminergic neurons.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

TOP2B_3877 (e-PCR)
- UniSTS:462594

Rarb (e-PCR), detects polymorphism
- UniSTS:470565

Top2b (e-PCR), detects polymorphism
- UniSTS:526911

D14Mit244 (e-PCR), detects polymorphism
- UniSTS:141981

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA negative supercoiling activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	ISS Inferred from Sequence or Structural Similarity more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	ISO Inferred from Sequence Orthology more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	ISS Inferred from Sequence or Structural Similarity more info
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonucleoprotein complex binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in B cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in DNA topological change	ISO Inferred from Sequence Orthology more info
involved_in DNA topological change	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to ATP	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hydrogen peroxide	IEA Inferred from Electronic Annotation more info
involved_in cellular senescence	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within forebrain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of double-strand break repair via nonhomologous end joining	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate	ISO Inferred from Sequence Orthology more info
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
NOT located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
colocalizes_with heterochromatin	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA topoisomerase 2-beta

Names: DNA topoisomerase II, beta isozyme

NP_033435.2

EC 5.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009409.3 → NP_033435.2 DNA topoisomerase 2-beta

See identical proteins and their annotated locations for NP_033435.2

Status: VALIDATED

Source sequence(s)

AC154452, AC154471

Consensus CDS

CCDS26833.1

UniProtKB/Swiss-Prot

Q64511, Q7TQG4

Related

ENSMUSP00000017629.5, ENSMUST00000017629.12

Conserved Domains (8) summary

cd03365
Location:464 → 584

TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...

PHA03151
Location:1344 → 1582

PHA03151; hypothetical protein; Provisional

cd00187
Location:720 → 1180

TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...

cd03481
Location:274 → 426

TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...

PTZ00108
Location:34 → 1457

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam02518
Location:89 → 186

HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

pfam08070
Location:1495 → 1596

DTHCT; DTHCT (NUC029) region

pfam16898
Location:582 → 720

TOPRIM_C; C-terminal associated domain of TOPRIM