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    top2a DNA topoisomerase II alpha [ Danio rerio (zebrafish) ]

    Gene ID: 323733, updated on 9-Dec-2024

    Summary

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    Official Symbol
    top2aprovided by ZNC
    Official Full Name
    DNA topoisomerase II alphaprovided by ZNC
    Primary source
    ZFIN:ZDB-GENE-030131-2453
    See related
    Ensembl:ENSDARG00000024488 AllianceGenome:ZFIN:ZDB-GENE-030131-2453
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Danio rerio
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
    Also known as
    fc08c07; wu:fc08c07
    Summary
    Predicted to enable DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity. Acts upstream of or within animal organ regeneration and chromosome segregation. Predicted to be located in nucleolus and nucleoplasm. Predicted to be part of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex. Predicted to be active in nucleus. Is expressed in several structures, including digestive system; eye; midbrain; neural tube; and pectoral fin. Orthologous to human TOP2A (DNA topoisomerase II alpha). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    human mouse all
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    Genomic context

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    See top2a in Genome Data Viewer
    Location:
    chromosome: 12
    Exon count:
    35
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCz11 (GCF_000002035.6) 12 NC_007123.7 (10706772..10740236)
    105 previous assembly GRCz10 (GCF_000002035.5) 12 NC_007123.6 (10668469..10701933)

    Chromosome 12 - NC_007123.7Genomic Context describing neighboring genes Neighboring gene mediator complex subunit 24 Neighboring gene sterile alpha motif domain containing 14 Neighboring gene retinoic acid receptor, alpha a Neighboring gene transfer RNA threonine (anticodon CGU) Neighboring gene VPS35 endosomal protein sorting factor like

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
    • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
    • BioProject: PRJEB1986
    • Analysis date: Fri Dec 8 19:48:10 2017

    Bibliography

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    Related articles in PubMed

    1. Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish. Dovey M, et al. Mol Cell Biol, 2009 Jul. PMID 19380487, Free PMC Article
    2. Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1. Sadler KC, et al. Proc Natl Acad Sci U S A, 2007 Jan 30. PMID 17242348, Free PMC Article
    3. Cyclin A2/cyclin-dependent kinase 1-dependent phosphorylation of Top2a is required for S phase entry during retinal development in zebrafish. Jin M, et al. J Genet Genomics, 2021 Jan 20. PMID 33832859
    4. Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development. Hale LA, et al. Gene Expr Patterns, 2006 Jun. PMID 16455309
    5. lin9 is required for mitosis and cell survival during early zebrafish development. Kleinschmidt MA, et al. J Biol Chem, 2009 May 8. PMID 19278998, Free PMC Article

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The absence of zygotic top2a is not fully compensated for by maternal, zygotic or ectopic top2b mRNA suggesting distinct functions of Top2a and Top2b in embryonic development.
      Title: Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
    2. Results confirm a conserved role for topoisomerase II alpha (TOP2A) in vertebrates as well as a dose-sensitive requirement for top2a in adults.
      Title: Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish.
    Submit: New GeneRIF Correction

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC92574

    Gene Ontology Provided by ZFIN

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on DNA ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA topological change IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA topological change IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA topological change ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    acts_upstream_of_or_within animal organ regeneration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within chromosome condensation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    acts_upstream_of_or_within chromosome segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within chromosome segregation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    acts_upstream_of_or_within mitotic S phase IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in resolution of meiotic recombination intermediates IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within rhythmic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sister chromatid segregation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    DNA topoisomerase 2-alpha
    Names
    topoisomerase (DNA) II alpha
    topoisomerase 2
    NP_001296944.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001310015.1 → NP_001296944.1  DNA topoisomerase 2-alpha

      Status: VALIDATED

      Source sequence(s)
      BX470262, EB928159, EH541591, EH551427, FO704786
      UniProtKB/TrEMBL
      A0A0R4IUF0, A0A8M1P7U6
      Related
      ENSDARP00000139353.2, ENSDART00000158227.2
      Conserved Domains (7) summary
      cd03365
      Location:454 → 574
      TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
      cd00187
      Location:710 → 1166
      TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
      cd03481
      Location:264 → 416
      TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
      PTZ00108
      Location:25 → 1440
      PTZ00108; DNA topoisomerase 2-like protein; Provisional
      pfam02518
      Location:79 → 173
      HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
      pfam08070
      Location:1468 → 1560
      DTHCT; DTHCT (NUC029) region
      pfam16898
      Location:572 → 710
      TOPRIM_C; C-terminal associated domain of TOPRIM

    RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCz11 Primary Assembly

    Genomic

    1. NC_007123.7 Reference GRCz11 Primary Assembly

      Range
      10706772..10740236
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001003834.1: Suppressed sequence

      Description
      NM_001003834.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.
    Nucleotide Protein Strain
    Heading Accession and Version

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