Ncan neurocan [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ncanprovided by MGI
Official Full Name: neurocanprovided by MGI
Primary source: MGI:MGI:104694
See related: Ensembl:ENSMUSG00000002341 AllianceGenome:MGI:104694
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cspg3; Tgfbit; Cspg3-rs; C230035B04
Summary: Predicted to enable calcium ion binding activity; carbohydrate binding activity; and hyaluronic acid binding activity. Involved in modulation of chemical synaptic transmission and regulation of postsynapse organization. Acts upstream of or within regulation of synapse structural plasticity. Is active in GABA-ergic synapse; glutamatergic synapse; and perisynaptic extracellular matrix. Is expressed in lower jaw; nervous system; and sensory organ. Orthologous to human NCAN (neurocan). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in whole brain E14.5 (RPKM 31.9), CNS E18 (RPKM 28.5) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 B3.3; 8 34.15 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (70545735..70573494, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (70093085..70120863, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Electroacupuncture promotes the repair of the damaged spinal cord in mice by mediating neurocan-perineuronal net.
Title: Electroacupuncture promotes the repair of the damaged spinal cord in mice by mediating neurocan-perineuronal net.
Neurocan Contributes to Perineuronal Net Development.
Title: Neurocan Contributes to Perineuronal Net Development.
Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses.
Title: Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses.
The synaptic plasticity occurs in the hippocampus of freely behaving mice that lack neurocan, the informational content stored by synaptic plasticity is not the same.
Title: Intrinsic cellular and molecular properties of in vivo hippocampal synaptic plasticity are altered in the absence of key synaptic matrix molecules.
Study shows the viability and proliferation of astrocytes are significantly increased by beta-amyloid in a dose dependent manner. Moreover, beta-amyloid may effectively up-regulate the neurocan expression via regulating Sox9.
Title: β-amyloid increases neurocan expression through regulating Sox9 in astrocytes: A potential relationship between Sox9 and chondroitin sulfate proteoglycans in Alzheimer's disease.
This study provided evidence that the neurocan modulates synaptogenesis and long-term synapse stability. The mutant neurons display reduced frequencies of mEPSCs and mIPSCs.
Title: Primary hippocampal neurons, which lack four crucial extracellular matrix molecules, display abnormalities of synaptic structure and function and severe deficits in perineuronal net formation.
Traumatic brain injury results in an increase in the chondroitin glycosaminoglycans (CSPGs) neurocan and NG2 expression in a tight band surrounding the injury core.
Title: Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice.
Ncan -/- mice showed behavioral abnormalities that were strikingly similar to those of the human mania phenotype.
Title: Studies in humans and mice implicate neurocan in the etiology of mania.
neurocan contributes to the molecular heterogeneity of the perinodal matrix, and its nodal expression is dependent on Bral1.
Title: Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
PTPsigma binds with high affinity to neural chondroitin sulfate proteoglycans(CPSGs); binding involves chondroitin sulfate chains & a specific site on 1st immunoglobulin-like domain of PTPsigma; results indicate PTPsigma can act as a receptor for CSPGs
Title: PTPsigma is a receptor for chondroitin sulfate proteoglycan, an inhibitor of neural regeneration.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:225364

Cspg3 (e-PCR), detects polymorphism
- UniSTS:141436

BE136470 (e-PCR)
- UniSTS:208292

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables hyaluronic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in central nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynapse organization	EXP Inferred from Experiment more info	PubMed
involved_in regulation of postsynapse organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of synapse structural plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in skeletal system development	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in perineuronal net	IBA Inferred from Biological aspect of Ancestor more info
located_in perineuronal net	ISO Inferred from Sequence Orthology more info
is_active_in perisynaptic extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info
is_active_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neurocan core protein

Names: TGFbeta-induced transcript, brain; chondroitin sulfate proteoglycan 3, related sequence

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007789.3 → NP_031815.2 neurocan core protein precursor

Status: VALIDATED

Source sequence(s)

BC065118, BQ745524, CX202471

Consensus CDS

CCDS22358.1

UniProtKB/Swiss-Prot

P55066, Q6P1E3, Q8C4F8

UniProtKB/TrEMBL

A0A0R4IZX5

Related

ENSMUSP00000002412.9, ENSMUST00000002412.9

Conserved Domains (7) summary

cd00033
Location:1167 → 1224

CCP; Complement control protein (CCP) modules (aka short consensus repeats SCRs or SUSHI repeats) have been identified in several proteins of the complement system

cd00054
Location:998 → 1034

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd03517
Location:159 → 253

Link_domain_CSPGs_modules_1_3; Link_domain_CSPGs_modules_1_3; this extracellular link domain is found in the first and third link modules of the chondroitin sulfate proteoglycan core protein (CSPG) aggrecan. In addition, it is found in the first link module of three other CSPGs: ...

cd03520
Location:260 → 355

Link_domain_CSPGs_modules_2_4; Link_domain_CSPGs_modules_2_4; this link domain is found in the second and fourth link modules of the chondroitin sulfate proteoglycan core protein (CSPG) aggrecan and, in the second link module of three other CSPGs: versican, neurocan, and brevican. The ...

pfam00008
Location:964 → 994

EGF; EGF-like domain

cl02432
Location:1040 → 1163

CLECT; C-type lectin (CTL)/C-type lectin-like (CTLD) domain

cl11960
Location:50 → 160

Ig; Immunoglobulin domain