Dgkb diacylglycerol kinase, beta [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dgkbprovided by RGD
Official Full Name: diacylglycerol kinase, betaprovided by RGD
Primary source: RGD:2488
See related: EnsemblRapid:ENSRNOG00000030771 AllianceGenome:RGD:2488
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Dagk; DGK-beta
Summary: Enables ATP-dependent diacylglycerol kinase activity. Involved in diacylglycerol metabolic process; lipid phosphorylation; and phosphatidic acid biosynthetic process. Is active in glutamatergic synapse. Is extrinsic component of postsynaptic density membrane. Orthologous to human DGKB (diacylglycerol kinase beta). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 223.5), Heart (RPKM 36.9) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q21

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (60368101..61124420)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (54640948..55397210)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (57516351..58277385)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study demonistrated that dgkb distribuate in rat cerebellum.
Title: Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
The DGKbeta localize in the outer plexiform layer, within which photoreceptor cells make contact with bipolar and horizontal cells.
Title: Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
functional roles of DGK in the pituitary intermediate lobe
Title: Signaling cascade of diacylglycerol kinase β in the pituitary intermediate lobe: dopamine D2 receptor/phospholipase Cβ4/diacylglycerol kinase β/protein kinase Cα.
In the hippocampus, DGKbeta is expressed in both projection neurons and interneurons and is accumulated at the perisynapse of dendritic spines in asymmetrical synapses. It may be involved in dendrite outgrowth and spinogenesis.
Title: Diacylglycerol kinase beta promotes dendritic outgrowth and spine maturation in developing hippocampal neurons.
Data suggest that abundant diacylglycerol kinase beta is present perisynaptically in medium spiny neurons so that it can effectively produce phosphatidic acid upon activation of Gq-coupled receptors and modulate striatal output neurons.
Title: Diacylglycerol kinase beta accumulates on the perisynaptic site of medium spiny neurons in the striatum.
These results suggest that DGKepsilon and DGKzeta play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.
Title: Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy.
In most brain regions, DGKbeta showed sustained expression throughout the postnatal developmental periods. And In the hippocampal pyramidal cell, DGKbeta localized along the membrane structure.
Title: Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D6Arb11 (e-PCR)
- UniSTS:239412

AU049028 (e-PCR)
- UniSTS:145476

RH144556 (e-PCR)
- UniSTS:233801

BF400076 (e-PCR)
- UniSTS:244827

S0226 (e-PCR)
- UniSTS:253032

RH135215 (e-PCR)
- UniSTS:218492

BF386108 (e-PCR)
- UniSTS:243586

RH94439 (e-PCR)
- UniSTS:119687

RH127545 (e-PCR)
- UniSTS:210855

D6Rat26 (e-PCR)
- UniSTS:118965

BF386309 (e-PCR)
- UniSTS:243686

D6Got70 (e-PCR)
- UniSTS:111604

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables lipid binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycerolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynapse organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynapse organization	ISO Inferred from Sequence Orthology more info
involved_in response to bacterium	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in extrinsic component of postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
NOT located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: diacylglycerol kinase beta

Names: 90 kDa diacylglycerol kinase; DAG kinase beta; Diacylglycerol kinase 90kDa; diacylglycerol kinase beta, 90kDa; diglyceride kinase beta

NP_062177.2

EC 2.7.1.107

XP_008762871.2

EC 2.7.1.107

XP_017449817.1

EC 2.7.1.107

XP_017449818.1

EC 2.7.1.107

XP_017449819.1

EC 2.7.1.107

XP_063118430.1

EC 2.7.1.107

XP_063118431.1

EC 2.7.1.107

XP_063118432.1

EC 2.7.1.107

XP_063118433.1

EC 2.7.1.107

XP_063118434.1

EC 2.7.1.107

XP_063118435.1

EC 2.7.1.107

XP_063118436.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019304.2 → NP_062177.2 diacylglycerol kinase beta

Status: VALIDATED

Source sequence(s)

JAXUCZ010000006

UniProtKB/Swiss-Prot

P49621

UniProtKB/TrEMBL

A6HBB8, F1LP01

Related

ENSRNOP00000058842.2, ENSRNOT00000063874.5

Conserved Domains (6) summary

smart00045
Location:580 → 760

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:438 → 559

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20845
Location:237 → 302

C1_DGKbeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins

cd20891
Location:307 → 365

C1_DGKbeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

60368101..61124420

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063262362.1 → XP_063118432.1 diacylglycerol kinase beta isoform X4

UniProtKB/TrEMBL

A0A8I5ZWT2
XM_017594328.3 → XP_017449817.1 diacylglycerol kinase beta isoform X1

UniProtKB/Swiss-Prot

P49621

Conserved Domains (6) summary

smart00045
Location:581 → 761

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:439 → 560

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20845
Location:237 → 302

C1_DGKbeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins

cd20891
Location:307 → 365

C1_DGKbeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins
XM_063262364.1 → XP_063118434.1 diacylglycerol kinase beta isoform X5
XM_017594330.3 → XP_017449819.1 diacylglycerol kinase beta isoform X3

UniProtKB/Swiss-Prot

P49621
XM_063262366.1 → XP_063118436.1 diacylglycerol kinase beta isoform X6
XM_063262360.1 → XP_063118430.1 diacylglycerol kinase beta isoform X2

UniProtKB/TrEMBL

A6HBB8, F1LP01
XM_017594329.3 → XP_017449818.1 diacylglycerol kinase beta isoform X3

UniProtKB/Swiss-Prot

P49621

Related

ENSRNOP00000082015.2, ENSRNOT00000111621.2
XM_008764649.4 → XP_008762871.2 diacylglycerol kinase beta isoform X1

UniProtKB/Swiss-Prot

P49621

Conserved Domains (6) summary

smart00045
Location:581 → 761

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:439 → 560

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00051
Location:152 → 221

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam14513
Location:6 → 148

DAG_kinase_N; Diacylglycerol kinase N-terminus

cd20845
Location:237 → 302

C1_DGKbeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins

cd20891
Location:307 → 365

C1_DGKbeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase beta (DAG kinase beta) and similar proteins
XM_063262363.1 → XP_063118433.1 diacylglycerol kinase beta isoform X5
XM_063262361.1 → XP_063118431.1 diacylglycerol kinase beta isoform X4

UniProtKB/TrEMBL

A0A8I5ZWT2

Related

ENSRNOP00000106123.1, ENSRNOT00000134799.1
XM_063262365.1 → XP_063118435.1 diacylglycerol kinase beta isoform X6