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    Mylk3 myosin light chain kinase 3 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 291926, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Mylk3provided by RGD
    Official Full Name
    myosin light chain kinase 3provided by RGD
    Primary source
    RGD:1305801
    See related
    EnsemblRapid:ENSRNOG00000017546 AllianceGenome:RGD:1305801
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    MLCK; RGD1305801
    Summary
    Enables calcium/calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in myofibril assembly; positive regulation of sarcomere organization; and protein phosphorylation. Located in cytosol. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Restricted expression toward (RPKM 1854.5) See more
    Orthologs
    human mouse all
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    Genomic context

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    See Mylk3 in Genome Data Viewer
    Location:
    19q11
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 19 NC_086037.1 (37858298..37916805)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 19 NC_051354.1 (21685085..21743587)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 19 NC_005118.4 (27388163..27442451)

    Chromosome 19 - NC_086037.1Genomic Context describing neighboring genes Neighboring gene similar to human chromosome 16 open reading frame 87 Neighboring gene ribosomal protein S11, pseudogene 2 Neighboring gene uncharacterized LOC134483311 Neighboring gene origin recognition complex, subunit 6 Neighboring gene U6 spliceosomal RNA Neighboring gene VPS35 retromer complex component

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK. Ávila-Rodríguez D, et al. Exp Cell Res, 2017 May 1. PMID 28300565
    2. CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes. Eikemo H, et al. Biochem Biophys Res Commun, 2016 Feb 26. PMID 26809094
    3. Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion. Gao L, et al. J Mol Cell Cardiol, 2014 Dec. PMID 25451385
    4. Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability. Al-Sadi R, et al. J Immunol, 2008 Apr 15. PMID 18390750, Free PMC Article
    5. Identification of cardiac-specific myosin light chain kinase. Chan JY, et al. Circ Res, 2008 Mar 14. PMID 18202317, Free PMC Article

    See all (8) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We found that a rounded shape with high cortical tension depends on MLCK and ROCK, whereas in cells with an elongated shape, MLCK is the primary protein responsible for cell spreading. Further, in cells with a slow and directionally persistent motility, MLCK predominates, while rapid and erratic movement is ROCK-dependent
      Title: The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK.
    2. In conclusion, in addition to MLCK, CaMKII phosphorylates RLC in cardiomyocytes.
      Title: CaMKII in addition to MLCK contributes to phosphorylation of regulatory light chain in cardiomyocytes.
    3. Ischemia/reperfusion-activated MMP-2 leads to the degradation of cardiac MLCK.
      Title: Degradation of cardiac myosin light chain kinase by matrix metalloproteinase-2 contributes to myocardial contractile dysfunction during ischemia/reperfusion.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium/calmodulin-dependent protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables calcium/calmodulin-dependent protein kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables calcium/calmodulin-dependent protein kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables myosin light chain kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables myosin light chain kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables myosin light chain kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cardiac myofibril assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cardiac myofibril assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to interleukin-1 ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of membrane permeability IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of membrane permeability ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of sarcomere organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of tight junction disassembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of tight junction disassembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in sarcomere organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in sarcomere organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sarcomere organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in sarcomerogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    colocalizes_with actin cytoskeleton IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in actin cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    myosin light chain kinase 3
    Names
    cardiac-MLCK
    cardiac-MyBP-C-associated Ca/CaM kinase
    putative myosin light chain kinase 3
    NP_001104280.1
    XP_006255417.1
    XP_038953507.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001110810.1 → NP_001104280.1  myosin light chain kinase 3

      See identical proteins and their annotated locations for NP_001104280.1

      Status: PROVISIONAL

      Source sequence(s)
      EU236721
      UniProtKB/Swiss-Prot
      A8WE96, E9PT87
      Related
      ENSRNOP00000023657.5, ENSRNOT00000023657.8
      Conserved Domains (2) summary
      smart00220
      Location:485 → 737
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14192
      Location:477 → 737
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086037.1 Reference GRCr8

      Range
      37858298..37916805
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039097579.2 → XP_038953507.1  myosin light chain kinase 3 isoform X2

      Conserved Domains (1) summary
      cd14192
      Location:198 → 458
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

    2. XM_006255355.4 → XP_006255417.1  myosin light chain kinase 3 isoform X1

      UniProtKB/TrEMBL
      A0A8I5ZYT9
      Related
      ENSRNOP00000083206.1, ENSRNOT00000100814.2
      Conserved Domains (2) summary
      smart00220
      Location:352 → 604
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14192
      Location:344 → 604
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    E9PT87.1 GenPept UniProtKB/Swiss-Prot:E9PT87

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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