ChAT Choline acetyltransferase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: ChATprovided by FlyBase
Official Full Name: Choline acetyltransferaseprovided by FlyBase
Primary source: FLYBASE:FBgn0000303
Locus tag: Dmel_CG12345
See related: AllianceGenome:FB:FBgn0000303
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Cat; CG12345; cha; Cha; ChA; chat; Chat; ChaT; ChAt; CHAT; ChAT4B1; CLAT_DROME; CT23399; dChAT; DChAT; Dmel\CG12345; l(3)91Cc
Summary: Enables choline O-acetyltransferase activity. Involved in acetylcholine biosynthetic process and neuromuscular synaptic transmission. Located in terminal bouton. Is expressed in several structures, including ganglia; neurons; sense organ; synaptic neuropil domain; and synaptic neuropil subdomain. Human ortholog(s) of this gene implicated in Alzheimer's disease and congenital myasthenic syndrome 6. Orthologous to human CHAT (choline O-acetyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 91C1-91C5; 3-64 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (18705501..18732344)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (14531223..14558066)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of B52 increases growth of axon branches. B52 function is also required for Choline acetyltransferase (ChAT ) splicing.
Title: Single neuron transcriptomics identify SRSF/SR protein B52 as a regulator of axon growth and Choline acetyltransferase splicing.
ChAT and GFP::ChAT directly bind to the KLP64D tail, which is essential for the GFP::ChAT entry and anterograde flow in axon.
Title: Interaction with a kinesin-2 tail propels choline acetyltransferase flow towards synapse.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 18722691-18723318 mobile_element_type = transposon:1360{}4813

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables choline O-acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline O-acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables choline O-acetyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in acetylcholine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuromuscular synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	NAS Non-traceable Author Statement more info	PubMed
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: choline acetyltransferase

Names: CG12345-PA; CG12345-PB; ChAT-PA; ChAT-PB; Choline-acetyltransferase; cholinacetyltransferase; choline acetyl transferase; choline-O-acetyltransferase

NP_477004.5

EC 2.3.-.-

EC 2.3.1.6

non-AUG (GUG) translation initiation

NP_996239.2

EC 2.3.-.-

EC 2.3.1.6

non-AUG (GUG) translation initiation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

18705501..18732344

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057656.3 → NP_477004.5 choline acetyltransferase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477004.5

Status: REVIEWED

UniProtKB/Swiss-Prot

P07668, Q8MQR2, Q9TXC6, Q9VE41

Related

FBpp0088397

Conserved Domains (1) summary

pfam00755
Location:88 → 716

Carn_acyltransf; Choline/Carnitine o-acyltransferase
NM_206517.2 → NP_996239.2 choline acetyltransferase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996239.2

Status: REVIEWED

UniProtKB/Swiss-Prot

P07668

Conserved Domains (1) summary

pfam00755
Location:88 → 709

Carn_acyltransf; Choline/Carnitine o-acyltransferase