gwl greatwall [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: gwlprovided by FlyBase
Official Full Name: greatwallprovided by FlyBase
Primary source: FLYBASE:FBgn0260399
Locus tag: Dmel_CG7719
See related: AllianceGenome:FB:FBgn0260399
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 1J; anon-WO2004063362.73; CG7719; CG7719-related; CT23435; Dmel\CG7719; EP0515; Gwl; Pk91C; Pk?2; scant
Summary: Enables 14-3-3 protein binding activity and protein serine/threonine kinase activity. Involved in several processes, including chromosome condensation; female meiosis I; and regulation of cell cycle process. Located in cytoplasm and nuclear envelope. Is expressed in several structures, including central brain primordium; embryonic central brain; embryonic/larval midgut primordium; larval nervous system; and pole plasm. Human ortholog(s) of this gene implicated in thrombocytopenia. Orthologous to human MASTL (microtubule associated serine/threonine kinase like). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 91C5-91C6; 3-64 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (18746370..18750360)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (14572092..14576082)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Greatwall-Endos-PP2A/B55[Twins] network regulates translation and stability of maternal transcripts in the Drosophila oocyte-to-embryo transition.
Title: Greatwall-Endos-PP2A/B55(Twins) network regulates translation and stability of maternal transcripts in the Drosophila oocyte-to-embryo transition.
Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A.
Title: Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A.
Our results indicate that the cyclic changes in Gwl localization at mitotic entry and exit are directly regulated by the antagonistic cyclin B-Cdk1 and PP2A-Tws enzymes
Title: Spatial regulation of greatwall by Cdk1 and PP2A-Tws in the cell cycle.
This mechanism explains simultaneously how PP2A-B55 and Gwl together regulate phosphorylated Endos, and how phosphorylated Endos controls PP2A-B55.
Title: Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Nuclear localization of Greatwall is required for its function. Spatial regulation of Gwl at mitotic entry contributes to the mitotic switch.
Title: Cell cycle regulation of Greatwall kinase nuclear localization facilitates mitotic progression.
greatwall protein antagonizes protein phosphatase 2A function in both meiosis and mitosis
Title: PP2A-twins is antagonized by greatwall and collaborates with polo for cell cycle progression and centrosome attachment to nuclei in drosophila embryos.
Greatwall phosphorylates Endos at a single site, Ser68, and this is essential for Endos function
Title: Suppression of scant identifies Endos as a substrate of greatwall kinase and a negative regulator of protein phosphatase 2A in mitosis.
These results indicate that Gwl activity antagonizes Polo and thus identify an important regulatory interaction of the cell cycle.
Title: Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with Polo kinase.
Data show that mutations in the Drosophila gene greatwall cause improper chromosome condensation and delay cell cycle progression in larval neuroblasts.
Title: Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: greatwall

Names: CG7719-PA; CG7719-PB; gwl-PA; gwl-PB; protein kinase-like 91C; scott of the antarctic

NP_001262713.1

EC 2.7.11.1

NP_524860.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

18746370..18750360

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080121.4 → NP_524860.2 greatwall, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524860.2

Status: REVIEWED

UniProtKB/TrEMBL

Q95TN8, Q9VE35

Related

FBpp0083089

Conserved Domains (3) summary

smart00220
Location:57 → 205

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05610
Location:52 → 255

STKc_MASTL; Catalytic domain of the Serine/Threonine Kinase, Microtubule-associated serine/threonine-like kinase (also called greatwall kinase)

cl21453
Location:655 → 835

PKc_like; Protein Kinases, catalytic domain
NM_001275784.1 → NP_001262713.1 greatwall, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262713.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGX7

Conserved Domains (2) summary

smart00220
Location:57 → 205

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl21453
Location:52 → 255

PKc_like; Protein Kinases, catalytic domain