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    nwk nervous wreck [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 39052, updated on 9-Dec-2024

    Summary

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    Official Symbol
    nwkprovided by FlyBase
    Official Full Name
    nervous wreckprovided by FlyBase
    Primary source
    FLYBASE:FBgn0263456
    Locus tag
    Dmel_CG43479
    See related
    AllianceGenome:FB:FBgn0263456
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG43479; CG4641; CG4684; Dmel\CG43479; Nwk; NWK
    Old locus tag
    Dmel_CG4641; Dmel_CG4684
    Summary
    Enables phosphatidylinositol-3,4-bisphosphate binding activity and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including negative regulation of synaptic assembly at neuromuscular junction; neuromuscular synaptic transmission; and regulation of actin filament organization. Located in neuromuscular junction and recycling endosome. Is expressed in several structures, including adult head; ganglia; larval nervous system; type I bouton; and ventral midline of embryo. Orthologous to several human genes including FCHSD2 (FCH and double SH3 domains 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See nwk in Genome Data Viewer
    Location:
    67A1-67A1; 3-28 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (9137860..9145693)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (9130960..9138793)

    Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene boule Neighboring gene Dihydropteridine reductase Neighboring gene Unconventional SNARE in the ER 1 Neighboring gene mir-4940 stem loop Neighboring gene Resistant to dieldrin Neighboring gene Solute carrier family 45 member 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Effects of PI(4,5)P(2) concentration on the F-BAR domain membrane binding as revealed by coarse-grained simulations. Stanishneva-Konovalova TB, et al. Proteins, 2019 Jul. PMID 30803020
    2. Assembly of actin filaments and microtubules in Nwk F-BAR-induced membrane deformations. Kelley CF, et al. Commun Integr Biol, 2015 Mar-Apr. PMID 26478768, Free PMC Article
    3. BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission. Ukken FP, et al. J Cell Sci, 2016 Jan 1. PMID 26567222, Free PMC Article
    4. Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation. Kelley CF, et al. Cell Rep, 2015 Dec 22. PMID 26686642, Free PMC Article
    5. Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck. Stanishneva-Konovalova TB, et al. Proc Natl Acad Sci U S A, 2016 Sep 20. PMID 27601635, Free PMC Article

    See all (70) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This study examines the interactions of the F-BAR domain of nervous wreck (Nwk) with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 )-containing membranes using coarse-grained molecular dynamics.
      Title: Effects of PI(4,5)P(2) concentration on the F-BAR domain membrane binding as revealed by coarse-grained simulations.
    2. In Drosophila neurons, Nwk autoregulation restricts SH3a domain-dependent synaptopod formation, synaptic growth, and actin organization. Our results define structural rearrangements in Nwk that control F-BAR-membrane interactions as well as SH3 domain activities, and suggest that these two functions are tightly coordinated in vitro and in vivo.
      Title: Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
    3. SH3PX1 promotes neurotransmitter release presynaptically, at least in part through functional interactions with Nwk.
      Title: BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission.
    4. Nwk-induced scallops can be extended by cytoskeletal forces to produce protrusions at the plasma membrane.
      Title: Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck.
    5. The results define a presynaptic trafficking pathway mediated by SNX16, NWK, and the ESCRT complex that functions to control synaptic growth signaling at the interface between endosomal compartments.
      Title: A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
    6. Synaptic growth activated by growth factor signaling is controlled at an endosomal compartment via coordinated Nwk and Cdc42-dependent actin assembly.
      Title: Nervous wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables phosphatidylinositol-3,4-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-4,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phospholipid binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in membrane organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of synaptic assembly at neuromuscular junction IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of synaptic assembly at neuromuscular junction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neuromuscular synaptic transmission IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in neuromuscular synaptic transmission IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in receptor recycling IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of actin filament polymerization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of actin filament polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of synaptic assembly at neuromuscular junction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in neuromuscular junction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in neuromuscular junction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in recycling endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in recycling endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    nervous wreck
    Names
    CG43479-PB
    CG43479-PC
    CG43479-PD
    CG43479-PF
    CG43479-PG
    CG43479-PH
    CG43479-PI
    CG43479-PJ
    CG43479-PK
    CG43479-PL
    nwk-PB
    nwk-PC
    nwk-PD
    nwk-PF
    nwk-PG
    nwk-PH
    nwk-PI
    nwk-PJ
    nwk-PK
    nwk-PL

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_037436.4 Reference assembly

      Range
      9137860..9145693
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001259762.2 → NP_001246691.1  nervous wreck, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246691.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VSU8
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    2. NM_001300067.1 → NP_001286996.1  nervous wreck, isoform K [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286996.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      X2JC77
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    3. NM_001259763.2 → NP_001246692.1  nervous wreck, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246692.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9NF56
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    4. NM_140033.3 → NP_648290.3  nervous wreck, isoform G [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_648290.3

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VSU8
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    5. NM_001300065.1 → NP_001286994.1  nervous wreck, isoform I [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286994.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      M9NE66, M9NF56, M9NFQ1, Q9VSU8, X2J8V0, X2JAU8, X2JC77, X2JCQ1, X2JGF9
      Conserved Domains (4) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      PRK12323
      Location:725 → 956
      PRK12323; DNA polymerase III subunits gamma and tau; Provisional
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    6. NM_001300066.1 → NP_001286995.1  nervous wreck, isoform J [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286995.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      X2JGF9
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily

    7. NM_001300068.1 → NP_001286997.1  nervous wreck, isoform L [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286997.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      X2JCQ1
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:640 → 695
      SH3; Src Homology 3 domain superfamily

    8. NM_001259760.2 → NP_001246689.1  nervous wreck, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246689.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9NE66
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:640 → 695
      SH3; Src Homology 3 domain superfamily

    9. NM_001300064.1 → NP_001286993.1  nervous wreck, isoform H [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286993.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      X2J8V0
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:640 → 695
      SH3; Src Homology 3 domain superfamily

    10. NM_001259761.2 → NP_001246690.1  nervous wreck, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001246690.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9NFQ1
      Conserved Domains (3) summary
      cd11761
      Location:545 → 600
      SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
      cl12013
      Location:16 → 281
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
      cl17036
      Location:663 → 718
      SH3; Src Homology 3 domain superfamily
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9VSU9 Q9VSU9
    X2JAU8.1 X2JAU8

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