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    robo1 roundabout 1 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 37603, updated on 9-Dec-2024

    Summary

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    Official Symbol
    robo1provided by FlyBase
    Official Full Name
    roundabout 1provided by FlyBase
    Primary source
    FLYBASE:FBgn0005631
    Locus tag
    Dmel_CG13521
    See related
    AllianceGenome:FB:FBgn0005631
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    BEST:GM07780; CG13521; CT32892; D-Robo1; Dmel\CG13521; dRobo; dRobo1; robo; Robo; ROBO; robo-1; Robo-1; Robo1; robo1S; roundabout
    Summary
    Enables heparin binding activity. Involved in several processes, including heart development; neuron projection guidance; and regulation of cell migration. Located in several cellular components, including axonal growth cone; dendrite; and neuronal cell body. Part of Slit-Robo signaling complex. Is expressed in several structures, including CNS neuron; epithelium; larval longitudinal connective; sensory neurons; and synaptic neuropil domain. Used to study myoclonic-atonic epilepsy. Human ortholog(s) of this gene implicated in congenital nystagmus (multiple); hematologic cancer (multiple); panhypopituitarism; and vesicoureteral reflux. Orthologous to several human genes including ROBO1 (roundabout guidance receptor 1). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See robo1 in Genome Data Viewer
    Location:
    59A2-59A3; 2-101 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (22692524..22700970, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (18580029..18588475, complement)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR44763 Neighboring gene long non-coding RNA:CR44764 Neighboring gene uncharacterized protein Neighboring gene Odorant-binding protein 59a Neighboring gene Odorant-binding protein 58b

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Signaling from Within: Endocytic Trafficking of the Robo Receptor Is Required for Midline Axon Repulsion. Charron F. PLoS Genet, 2015 Sep. PMID 26334304, Free PMC Article
    2. Genetic dissection of a regionally differentiated network for exploratory behavior in Drosophila larvae. Berni J. Curr Biol, 2015 May 18. PMID 25959962, Free PMC Article
    3. Effects of roundabout on growth cone dynamics, filopodial length, and growth cone morphology at the midline and throughout the neuropile. Murray MJ, et al. J Neurosci, 1999 Sep 15. PMID 10479692, Free PMC Article
    4. Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity. Ahmed YA, et al. Mol Biosyst, 2016 Oct 20. PMID 27502551, Free PMC Article
    5. Axon guidance: crossing the midline. Thomas JB. Curr Biol, 1998 Jan 29. PMID 9443908

    See all (378) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
      Title: Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
    2. Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
      Title: Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
    3. Results indicate that each of the Drosophila Roundabout (Robo1) Fibronectin (Fn) repeats are individually dispensable for the protein's role in midline repulsion.
      Title: In Vivo Functional Analysis of Drosophila Robo1 Fibronectin Type-III Repeats.
    4. Vascular cells produce Slit that activates Robo receptors in the posterior signalling centre of the larval haematopoietic organ, the lymph gland.
      Title: Vascular control of the Drosophila haematopoietic microenvironment by Slit/Robo signalling.
    5. Mmy is required for Slit secretion and for maintaining Robo abundance and distribution in the developing nervous system in Drosophila.
      Title: The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons.
    6. Dscam1 appears to modify the output of Robo and Slit complexes so that signaling is no longer repulsive
      Title: Dscam1 Forms a Complex with Robo1 and the N-Terminal Fragment of Slit to Promote the Growth of Longitudinal Axons.
    7. loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells.
      Title: Drosophila Atg16 promotes enteroendocrine cell differentiation via regulation of intestinal Slit/Robo signaling.
    8. Findings indicate that four of the five immunoglobulin-like (Ig) domains within Drosophila Robo1 are dispensable for its role in midline repulsion, despite their strong evolutionary conservation, and highlight a unique requirement for the Slit-binding Ig1 domain in the regulation of midline crossing.
      Title: In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains.
    9. FruBM employs the Robo1 pathway for formation of the male-specific ipsilateral neurite
      Title: Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila.
    10. Deleting Ig1 from Robo1 disrupts Slit binding in cultured Drosophila cells. Robo1 variant lacking Ig1 (Robo1Ig1) is unable to promote ectopic midline repulsion.
      Title: Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo.
    Submit: New GeneRIF Correction See all GeneRIFs (40)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables axon guidance receptor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables heparin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Roundabout signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in Roundabout signaling pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in axon guidance IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in axon guidance IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in axon midline choice point recognition IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in axon midline choice point recognition IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in axon midline choice point recognition IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dendrite guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dendrite morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in embryonic heart tube development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in entrainment of circadian clock IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in gonad development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in motor neuron axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in muscle cell cellular homeostasis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in neuron recognition IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in outflow tract morphogenesis IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in photoreceptor cell axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of cell-cell adhesion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of dendrite development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of epithelial cell migration, open tracheal system IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in salivary gland boundary specification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Slit-Robo signaling complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in axon IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in axonal growth cone IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in dendrite IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in neuronal cell body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    part_of protein-containing complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    roundabout 1
    Names
    CG13521-PA
    CG13521-PC
    robo
    robo1-PA
    robo1-PC
    roundabout

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      22692524..22700970 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_057551.4 → NP_476899.1  roundabout 1, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_476899.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      O44924, Q7KVK3, Q9W213
      Related
      FBpp0071833
      Conserved Domains (8) summary
      cd05724
      Location:159 → 251
      Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      cd05725
      Location:272 → 341
      Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      smart00408
      Location:459 → 521
      IGc2; Immunoglobulin C-2 Type
      smart00410
      Location:351 → 436
      IG_like; Immunoglobulin like
      cd00063
      Location:549 → 637
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:56 → 150
      I-set; Immunoglobulin I-set domain
      pfam00041
      Location:770 → 855
      fn3; Fibronectin type III domain
      cl11960
      Location:56 → 151
      Ig; Immunoglobulin domain

    2. NM_166543.2 → NP_726224.3  roundabout 1, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_726224.3

      Status: REVIEWED

      UniProtKB/TrEMBL
      O44924, Q7KVK3, Q9W213
      Conserved Domains (8) summary
      cd05724
      Location:159 → 251
      Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      cd05725
      Location:272 → 341
      Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      smart00408
      Location:459 → 521
      IGc2; Immunoglobulin C-2 Type
      smart00410
      Location:351 → 436
      IG_like; Immunoglobulin like
      cd00063
      Location:549 → 637
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:56 → 150
      I-set; Immunoglobulin I-set domain
      pfam00041
      Location:770 → 855
      fn3; Fibronectin type III domain
      cl11960
      Location:56 → 151
      Ig; Immunoglobulin domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O44924 O44924
    Q9W213 Q9W213

    Gene LinkOut

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