Sh Shaker [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Shprovided by FlyBase
Official Full Name: Shakerprovided by FlyBase
Primary source: FLYBASE:FBgn0003380
Locus tag: Dmel_CG12348
See related: AllianceGenome:FB:FBgn0003380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA:GH03046; CG12348; CG17860; CG7640; Dmel\CG12348; Eko; EKO; F-c; Kv1; K[[v]]1; minisleep; mns; sh; SH; sha; Sha; Shw; V-I
Summary: Enables voltage-gated monoatomic cation channel activity. Involved in several processes, including mating behavior, sex discrimination; proboscis extension reflex; and regulation of synaptic activity. Located in membrane. Part of voltage-gated potassium channel complex. Is expressed in several structures, including adult antennal nerve; adult head; adult somatic muscle cell; mushroom body; and synaptic neuropil domain. Used to study epilepsy and episodic ataxia type 1. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 32; episodic ataxia type 1; familial atrial fibrillation; and pulmonary hypertension. Orthologous to several human genes including KCNA2 (potassium voltage-gated channel subfamily A member 2) and KCNA3 (potassium voltage-gated channel subfamily A member 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 16F3-16F6; 1-59 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (17924307..18063247, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (17818340..17957278, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission.
Title: The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission.
Molecular and cellular correlates in Kv channel clustering: entropy-based regulation of cluster ion channel density.
Title: Molecular and cellular correlates in Kv channel clustering: entropy-based regulation of cluster ion channel density.
S4-S5 linker movement during activation and inactivation in voltage-gated K(+) channels.
Title: S4-S5 linker movement during activation and inactivation in voltage-gated K(+) channels.
Authors provide evidence that in the Drosophila Shaker voltage-gated K(+) channels the S3 domain acts as an extracellular hydrophobic 'stabilizer' for the S4 domain biasing the gating chemical equilibrium toward the open state.
Title: Energetic role of the paddle motif in voltage gating of Shaker K(+) channels.
This study demonstrates that Shaker is not always a good model for Kv1 channels for ligand binding.
Title: Why the Drosophila Shaker K+ channel is not a good model for ligand binding to voltage-gated Kv1 channels.
The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement.
Title: The shaker potassium channel is no target for xenon anesthesia in short-sleeping Drosophila melanogaster mutants.
Data show several strong Cysteine-Cadmium-Cysteine bridges in Shaker potassium channel.
Title: Tracking a complete voltage-sensor cycle with metal-ion bridges.
Synaptic transmission at the larval neuromuscular junction and the transient Shaker potassium current IA in larval muscles have been characterized.
Title: Modulation of the frequency response of Shaker potassium channels by the quiver peptide suggesting a novel extracellular interaction mechanism.
synaptic strength and synaptic strength at neuromuscular junctions along the dorsal-ventral differentials at physiological Ca(2+) levels were not significantly altered in slowpoke (slo) and Shaker (Sh) mutants
Title: Pre- and post-synaptic mechanisms of synaptic strength homeostasis revealed by slowpoke and shaker K+ channel mutations in Drosophila.
allosteric communication across disparate regions of the channel protein and between evolved and regulated amino acid changes introduced by RNA editing.
Title: Regulated RNA editing and functional epistasis in Shaker potassium channels.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:98954

NoName (e-PCR)
- UniSTS:98955

Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c18031365-18031261 mobile_element_type = transposon:INE-1{}4898

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables delayed rectifier potassium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables voltage-gated monoatomic cation channel activity	IDA Inferred from Direct Assay more info	PubMed
enables voltage-gated potassium channel activity	IEA Inferred from Electronic Annotation more info
enables voltage-gated potassium channel activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in action potential	IBA Inferred from Biological aspect of Ancestor more info
involved_in action potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in behavioral response to ether	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to dopamine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in courtship behavior	NAS Non-traceable Author Statement more info	PubMed
involved_in courtship behavior	TAS Traceable Author Statement more info	PubMed
involved_in detection of visible light	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in learning or memory	NAS Non-traceable Author Statement more info	PubMed
involved_in mating behavior, sex discrimination	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of circadian sleep/wake cycle, sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in potassium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transport	TAS Traceable Author Statement more info	PubMed
involved_in proboscis extension reflex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein homooligomerization	IEA Inferred from Electronic Annotation more info
involved_in regulation of circadian sleep/wake cycle, sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of taste	NAS Non-traceable Author Statement more info	PubMed
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of voltage-gated potassium channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of voltage-gated potassium channel complex	IEA Inferred from Electronic Annotation more info
part_of voltage-gated potassium channel complex	IPI Inferred from Physical Interaction more info	PubMed
part_of voltage-gated potassium channel complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: shaker

Names: CG12348-PA; CG12348-PB; CG12348-PC; CG12348-PD; CG12348-PE; CG12348-PF; CG12348-PH; CG12348-PI; CG12348-PJ; CG12348-PK; CG12348-PL; CG12348-PM; CG12348-PQ; CG12348-PS; CG12348-PT; CG12348-PU; CG12348-PV; F-c transcription unit; Sh-PA; Sh-PB; Sh-PC; Sh-PD; Sh-PE; Sh-PF; Sh-PH; Sh-PI; Sh-PJ; Sh-PK; Sh-PL; Sh-PM; Sh-PQ; Sh-PS; Sh-PT; Sh-PU; Sh-PV; Shaker-downheld; minisleep

NP_001259666.2

non-AUG (AUU) translation initiation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

17924307..18063247 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078669.5 → NP_523393.3 shaker, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523393.3

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Related

FBpp0088600

Conserved Domains (2) summary

cl38908
Location:97 → 223

BTB_POZ; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily

pfam00520
Location:226 → 488

Ion_trans; Ion transport protein
NM_001272737.1 → NP_001259666.2 shaker, isoform S [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

M9PHG6

Conserved Domains (4) summary

smart00225
Location:87 → 170

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:262 → 476

Ion_trans; Ion transport protein

pfam02214
Location:87 → 175

BTB_2; BTB/POZ domain

pfam07885
Location:391 → 467

Ion_trans_2; Ion channel
NM_167592.4 → NP_728120.1 shaker, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_728120.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Related

FBpp0088602

Conserved Domains (4) summary

smart00225
Location:54 → 137

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:229 → 443

Ion_trans; Ion transport protein

pfam02214
Location:54 → 142

BTB_2; BTB/POZ domain

pfam07885
Location:358 → 434

Ion_trans_2; Ion channel
NM_001414058.1 → NP_001401061.1 shaker, isoform U [Drosophila melanogaster]

Status: REVIEWED
NM_001272736.2 → NP_001259665.2 shaker, isoform Q [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Conserved Domains (2) summary

cl38908
Location:97 → 223

BTB_POZ; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily

pfam00520
Location:226 → 488

Ion_trans; Ion transport protein
NM_167594.4 → NP_728122.1 shaker, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_728122.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Related

FBpp0088603

Conserved Domains (4) summary

smart00225
Location:87 → 170

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:262 → 476

Ion_trans; Ion transport protein

pfam02214
Location:87 → 175

BTB_2; BTB/POZ domain

pfam07885
Location:391 → 467

Ion_trans_2; Ion channel
NM_001201739.2 → NP_001188668.1 shaker, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001188668.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Conserved Domains (4) summary

smart00225
Location:87 → 170

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:262 → 476

Ion_trans; Ion transport protein

pfam02214
Location:87 → 175

BTB_2; BTB/POZ domain

pfam07885
Location:391 → 467

Ion_trans_2; Ion channel
NM_206775.5 → NP_996498.2 shaker, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996498.2

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Conserved Domains (4) summary

smart00225
Location:87 → 170

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:262 → 476

Ion_trans; Ion transport protein

pfam02214
Location:87 → 175

BTB_2; BTB/POZ domain

pfam07885
Location:391 → 467

Ion_trans_2; Ion channel
NM_001258798.2 → NP_001245727.1 shaker, isoform L [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245727.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08510

Conserved Domains (4) summary

smart00225
Location:87 → 170

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:262 → 476

Ion_trans; Ion transport protein

pfam02214
Location:87 → 175

BTB_2; BTB/POZ domain

pfam07885
Location:391 → 467

Ion_trans_2; Ion channel
NM_001414059.1 → NP_001401062.1 shaker, isoform V [Drosophila melanogaster]

Status: REVIEWED
NM_167595.4 → NP_728123.1 shaker, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_728123.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V4Q2, B6IDJ2, M9MSC0, M9NEM4, M9PF21, P08510, P08511, P08512, P08513, Q24277, Q24521, Q7KUW5, Q8IQY9, Q8MT41, Q9VWZ5, Q9VWZ9, Q9VX00

Related

FBpp0088599

Conserved Domains (4) summary

smart00225
Location:99 → 182

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:274 → 488

Ion_trans; Ion transport protein

pfam02214
Location:99 → 187

BTB_2; BTB/POZ domain

pfam07885
Location:403 → 479

Ion_trans_2; Ion channel
NM_001316640.1 → NP_001303569.1 shaker, isoform T [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0S0X7Z4

Conserved Domains (4) summary

smart00225
Location:99 → 182

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:274 → 488

Ion_trans; Ion transport protein

pfam02214
Location:99 → 187

BTB_2; BTB/POZ domain

pfam07885
Location:403 → 479

Ion_trans_2; Ion channel
NM_167596.5 → NP_728124.1 shaker, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_728124.1

Status: REVIEWED

UniProtKB/TrEMBL

D5SHU7, E1JJQ5

Related

FBpp0088601

Conserved Domains (3) summary

smart00225
Location:54 → 137

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:229 → 283

Ion_trans; Ion transport protein

pfam02214
Location:54 → 142

BTB_2; BTB/POZ domain
NM_001272735.3 → NP_001259664.1 shaker, isoform M [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259664.1

Status: REVIEWED

UniProtKB/TrEMBL

D5SHU7, M9PJQ4

Conserved Domains (2) summary

cl38908
Location:85 → 211

BTB_POZ; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily

pfam00520
Location:214 → 316

Ion_trans; Ion transport protein
NM_001169317.1 → NP_001162788.1 shaker, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001162788.1

Status: REVIEWED

UniProtKB/TrEMBL

D5SHU7, E1JJQ5

Conserved Domains (3) summary

smart00225
Location:54 → 137

BTB; Broad-Complex, Tramtrack and Bric a brac

pfam00520
Location:229 → 283

Ion_trans; Ion transport protein

pfam02214
Location:54 → 142

BTB_2; BTB/POZ domain
NM_001258797.1 → NP_001245726.1 shaker, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001245726.1

Status: REVIEWED

UniProtKB/TrEMBL

D5SHU7, M9NGK2

Conserved Domains (2) summary

cl38908
Location:45 → 171

BTB_POZ; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily

pfam00520
Location:174 → 276

Ion_trans; Ion transport protein
NM_206774.2 → NP_996497.1 shaker, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996497.1

Status: REVIEWED

UniProtKB/TrEMBL

D5SHU7

Conserved Domains (2) summary

cl38908
Location:97 → 223

BTB_POZ; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily

pfam00520
Location:226 → 328

Ion_trans; Ion transport protein