egl-4 cGMP-dependent protein kinase;cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: egl-4
Official Full Name: cGMP-dependent protein kinase;cGMP-dependent protein kinase egl-4
Primary source: WormBase:WBGene00001173
Locus tag: CELE_F55A8.2
See related: AllianceGenome:WB:WBGene00001173
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables cGMP-dependent protein kinase activity. Involved in several processes, including chemotropism; enzyme-linked receptor protein signaling pathway; and regulation of signal transduction. Located in cytosol and nucleus. Is expressed in several structures, including marginal cell; nerve ring; neurons; spermatheca; and vulval muscle. Used to study ciliopathy. Human ortholog(s) of this gene implicated in thoracic aortic aneurysm. Orthologous to human PRKG1 (protein kinase cGMP-dependent 1). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See egl-4 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 15

Sequence:: Chromosome: IV; NC_003282.8 (1848071..1880596)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

EGL-4/PKG regulates the role of an interneuron in a chemotaxis circuit of C. elegans through mediating integration of sensory signals.
Title: EGL-4/PKG regulates the role of an interneuron in a chemotaxis circuit of C. elegans through mediating integration of sensory signals.
we provide evidence that the C. elegans transmembrane guanylyl cyclase ODR-1 functions in a non-cell-autonomous manner to provide cGMP to regulate EGL-4 function in ASH
Title: Aversive Behavior in the Nematode C. elegans Is Modulated by cGMP and a Neuronal Gap Junction Network.
Data (including data from studies in mutant and transgenic strains) suggest that stress response to fasting (lysosomal lipid accumulation) is co-ordinately regulated by EGL-4/PKG (protein kinase G) and nuclear hormone receptor NHR-49.
Title: PKG and NHR-49 signalling co-ordinately regulate short-term fasting-induced lysosomal lipid accumulation in C. elegans.
Nuclear PKG localization is necessary in the AWB neurons to mediate avoidance in Caenorhabditis elegans.
Title: Nuclear PKG localization is regulated by G₀ alpha and is necessary in the AWB neurons to mediate avoidance in Caenorhabditis elegans.
activated EGL-4 dampens quinine sensitivity via phosphorylation and activation of the regulator of G protein signaling (RGS) proteins RGS-2 and RGS-3, which in turn downregulate Galpha signaling and behavioral sensitivity.
Title: The C. elegans cGMP-dependent protein kinase EGL-4 regulates nociceptive behavioral sensitivity.
odor-induced fluctuations in cGMP levels and this fluctuation may be responsible for sending EGL-4 into the AWC nucleus.
Title: Changes in cGMP levels affect the localization of EGL-4 in AWC in Caenorhabditis elegans.
an effector of cGMP signalling, a cGMP-dependent protein kinase, EGL-4, also modifies bbs mutant defects
Title: Mutations in a guanylate cyclase GCY-35/GCY-36 modify Bardet-Biedl syndrome-associated phenotypes in Caenorhabditis elegans.
active EGL-4/PKG acts in the nucleus via a conserved Class I histone deacetylase complex to regulate gene expression pertinent to behavioral and physiological responses to cGMP
Title: Nuclear cGMP-dependent kinase regulates gene expression via activity-dependent recruitment of a conserved histone deacetylase complex.
EGL-4 protein kinase acts as a sensor that integrates odor signaling over time, and its nuclear translocation is an instructive switch that allows the animal to ignore persistent odors.
Title: Nuclear entry of a cGMP-dependent kinase converts transient into long-lasting olfactory adaptation.
The PKG homolog egl-4 promotes quiescence by acting at two levels: in the sensory neurons and also downstream of command interneurons.
Title: Calcineurin and protein kinase G regulate C. elegans behavioral quiescence during locomotion in liquid.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

F55A8.2 (e-PCR)
- UniSTS:314464

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	IEA Inferred from Electronic Annotation more info
enables cGMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cGMP-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables cyclic nucleotide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chemosensory behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chemosensory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chemotaxis	IEA Inferred from Electronic Annotation more info
involved_in chemotropism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in insulin receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in larval feeding behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cGMP-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of dauer larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of multicellular organism growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of organ growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in olfactory learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cGMP-mediated signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cGMP-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cellular response to alcohol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in receptor guanylyl cyclase signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in receptor guanylyl cyclase signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of eating behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of egg-laying behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of egg-laying behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of multicellular organism growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to alcohol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hydrogen peroxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to odorant	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxygen levels	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of bitter taste	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sensory perception of bitter taste	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: cGMP-dependent protein kinase;cGMP-dependent protein kinase egl-4

NP_001023223.1

Confirmed by transcript evidence

NP_001023224.1

Confirmed by transcript evidence

NP_001294450.1

Confirmed by transcript evidence

NP_001368271.1

Confirmed by transcript evidence

NP_500141.1

Confirmed by transcript evidence

NP_500142.1

Confirmed by transcript evidence

NP_741329.1

Confirmed by transcript evidence

NP_741330.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

1848071..1880596

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_067740.6 → NP_500141.1 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_500141.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360, Q688A8, Q688A9, Q7KPJ2, Q8MXG6, Q8MXG7

Conserved Domains (5) summary

PTZ00263
Location:458 → 776

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:731 → 780

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:195 → 316

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:319 → 434

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:475 → 736

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_001307521.5 → NP_001294450.1 cGMP-dependent protein kinase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001294450.1

Status: REVIEWED

UniProtKB/TrEMBL

U4PEQ9

Conserved Domains (5) summary

PTZ00263
Location:461 → 779

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:734 → 783

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:195 → 316

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:201 → 314

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:478 → 739

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_067741.5 → NP_500142.1 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_500142.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360

Conserved Domains (5) summary

PTZ00263
Location:415 → 733

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:688 → 737

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:152 → 273

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:276 → 391

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:432 → 693

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_001028052.5 → NP_001023223.1 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023223.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360

Conserved Domains (5) summary

PTZ00263
Location:421 → 739

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:694 → 743

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:158 → 279

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:282 → 397

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:438 → 699

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_171279.6 → NP_741329.1 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741329.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360

Conserved Domains (5) summary

PTZ00263
Location:427 → 745

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:700 → 749

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:164 → 285

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:288 → 403

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:444 → 705

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_171280.4 → NP_741330.2 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741330.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360

Conserved Domains (1) summary

cl00047
Location:79 → 126

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...
NM_001380068.1 → NP_001368271.1 cGMP-dependent protein kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

U4PRL6

Conserved Domains (3) summary

smart00133
Location:435 → 484

S_TK_X; Extension to Ser/Thr-type protein kinases

cd00038
Location:23 → 138

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:179 → 440

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_001028053.6 → NP_001023224.1 cGMP-dependent protein kinase egl-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023224.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O76360

Conserved Domains (5) summary

PTZ00263
Location:148 → 466

PTZ00263; protein kinase A catalytic subunit; Provisional

smart00133
Location:421 → 470

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:7 → 114

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:9 → 124

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:165 → 426

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)