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    sax-3 Protein sax-3 [ Caenorhabditis elegans ]

    Gene ID: 180637, updated on 9-Dec-2024

    Summary

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    Official Symbol
    sax-3
    Official Full Name
    Protein sax-3
    Primary source
    WormBase:WBGene00004729
    Locus tag
    CELE_ZK377.2
    See related
    AllianceGenome:WB:WBGene00004729
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Caenorhabditis elegans (strain: Bristol N2)
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
    Summary
    Enables axon guidance receptor activity. Involved in generation of neurons and regulation of sensory neuron axon guidance. Located in plasma membrane. Is expressed in several structures, including QR.a; QR.p; muscle cell; neuroblasts; and neurons. Human ortholog(s) of this gene implicated in congenital nystagmus (multiple); hematologic cancer (multiple); panhypopituitarism; and vesicoureteral reflux. Orthologous to human ROBO1 (roundabout guidance receptor 1); ROBO2 (roundabout guidance receptor 2); and ROBO3 (roundabout guidance receptor 3). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See sax-3 in Genome Data Viewer
    Location:
    chromosome: X
    Exon count:
    12
    Sequence:
    Chromosome: X; NC_003284.9 (3451908..3465927, complement)

    Chromosome X - NC_003284.9Genomic Context describing neighboring genes Neighboring gene FIP (Fungus-Induced Protein) Related Neighboring gene G-protein coupled receptors family 1 profile domain-containing protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene Uncharacterized protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003284.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans. Zallen JA, et al. Cell, 1998 Jan 23. PMID 9458046
    2. Structural Principles in Robo Activation and Auto-inhibition. Barak R, et al. Cell, 2019 Apr 4. PMID 30853216
    3. Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, et al. Dev Cell, 2019 Jan 28. PMID 30555000
    4. Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, et al. Dev Cell, 2019 May 6. PMID 31063761
    5. Axon guidance. A Roundabout way of avoiding the midline. Dickson B. Nature, 1998 Jan 29. PMID 9461209

    See all (89) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
      Title: Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
    2. Robo functions as an attractive cue for glial migration through SYG-1/Neph.
      Title: Robo functions as an attractive cue for glial migration through SYG-1/Neph.
    3. These data suggest that chw-1 is likely acting downstream and/or in parallel to sax-3 in axon guidance.
      Title: The Atypical Rho GTPase CHW-1 Works with SAX-3/Robo To Mediate Axon Guidance in Caenorhabditis elegans.
    4. SAX-3 binds to Wnt/CWN-2 and facilitates the membrane recruitment of CWN-2. SAX-3 forms a complex with the Ror/CAM-1 receptor and its downstream effector Dsh/DSH-1.
      Title: Robo and Ror function in a common receptor complex to regulate Wnt-mediated neurite outgrowth in Caenorhabditis elegans.
    5. SAX-3 regulate convergent extension-based nerve cord assembly in C. elegans
      Title: PCP and SAX-3/Robo Pathways Cooperate to Regulate Convergent Extension-Based Nerve Cord Assembly in C. elegans.
    6. We describe the effects that UNC-40 (DCC) and SAX-3 (Robo) receptors and the UNC-6, EGL-20, UNC-52, and SLT-1 extracellular cues have on directional bias of the axon outgrowth activity. the directional bias created by the cues depend on UNC-40 or SAX-3.
      Title: SAX-3 (Robo) and UNC-40 (DCC) regulate a directional bias for axon guidance in response to multiple extracellular cues.
    7. The SAX-3 receptor stimulates axon outgrowth and the signal sequence and transmembrane domain are critical for SAX-3 membrane localization in the posterior deirid neuron of C. elegans.
      Title: The SAX-3 receptor stimulates axon outgrowth and the signal sequence and transmembrane domain are critical for SAX-3 membrane localization in the PDE neuron of C. elegans.
    8. SAX-3 is required in the nervous system for proper extension of ventral anterior muscle processes.
      Title: Membrane extensions are associated with proper anterior migration of muscle cells during Caenorhabditis elegans embryogenesis.
    9. Axon outgrowth is not dependent on unc-40 or sax-3.
      Title: A developmental timing switch promotes axon outgrowth independent of known guidance receptors.
    10. EVA-1 identified as a transmembrane receptor for SLT-1; EVA-1 acts cell-autonomously for SLT-1/Slit-dependent axon migration functions of SAX-3/Robo, colocalizes with SAX-3 on cells & provides cell specificity to activation of SAX-3 signaling by SLT-1
      Title: The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by WormBase

    Function Evidence Code Pubs
    enables axon guidance receptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Roundabout signaling pathway ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    involved_in axon guidance IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cell-cell adhesion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in dorsal/ventral axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neuron migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of sensory neuron axon guidance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in sensory neuron axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in system development IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in axon IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Protein sax-3
    NP_001024990.1
    • Confirmed by transcript evidence
    NP_741748.2
    • Confirmed by transcript evidence

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003284.9 Reference assembly

      Range
      3451908..3465927 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001029819.7 → NP_001024990.1  Protein sax-3 [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_001024990.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      G5EBF1, H2KYQ9
      Conserved Domains (5) summary
      cd05724
      Location:137 → 223
      Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      smart00060
      Location:755 → 836
      FN3; Fibronectin type 3 domain
      cd00063
      Location:531 → 624
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:227 → 313
      I-set; Immunoglobulin I-set domain
      cl11960
      Location:30 → 129
      Ig; Immunoglobulin domain

    2. NM_171652.8 → NP_741748.2  Protein sax-3 [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_741748.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      G5EBF1
      Conserved Domains (5) summary
      cd05724
      Location:137 → 223
      Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors
      smart00060
      Location:755 → 836
      FN3; Fibronectin type 3 domain
      cd00063
      Location:531 → 624
      FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
      pfam07679
      Location:227 → 313
      I-set; Immunoglobulin I-set domain
      cl11960
      Location:30 → 129
      Ig; Immunoglobulin domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    G5EBF1.1

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