mbk-2 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2;dual-specificity kinase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: mbk-2
Official Full Name: Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2;dual-specificity kinase
Primary source: WormBase:WBGene00003150
Locus tag: CELE_F49E11.1
See related: AllianceGenome:WB:WBGene00003150
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables protein serine/threonine kinase activity and protein tyrosine kinase activity. Involved in several processes, including P granule disassembly; asymmetric protein localization involved in cell fate determination; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in several cellular components, including P granule; condensed chromosome; and microtubule cytoskeleton. Is expressed in several structures, including P0; body wall musculature; gonad; oocyte; and pharynx. Used to study Down syndrome. Orthologous to human DYRK2 (dual specificity tyrosine phosphorylation regulated kinase 2) and DYRK3 (dual specificity tyrosine phosphorylation regulated kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See mbk-2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 15

Sequence:: Chromosome: IV; NC_003282.8 (13009158..13035961)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development.
Title: Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development.
Phosphorylation by MBK-2 facilitates the binding of OMA proteins to TAF-4 and simultaneously inactivates their function in repressing zif-1 translation during the specification of primordial germ cells.
Title: zif-1 translational repression defines a second, mutually exclusive OMA function in germline transcriptional repression.
The pseudotyrosine phosphatases EGG-4 and EGG-5 sequester activated MBK-2 until the meiotic divisions by binding to the YTY motif and inhibiting MBK-2's kinase activity directly.
Title: Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition.
mbk-2 is essential in the zygote to ensure the postmeiotic inactivation of MEI-1 possibly via direct phosphorylation regulation
Title: The mbk-2 kinase is required for inactivation of MEI-1/katanin in the one-cell Caenorhabditis elegans embryo.
MBK-2 down-regulates the katanin-related protein MEI-1 to control spindle positioning and, through yet unknown factors, to control the localization of cytoplasmic determinants.
Title: The minibrain kinase homolog, mbk-2, is required for spindle positioning and asymmetric cell division in early C. elegans embryos.
MBK-2 directly phosphorylates MEI-1 and OMA-1 in vitro and that this activity is essential for their degradation in oocytes.
Title: The C. elegans DYRK Kinase MBK-2 Marks Oocyte Proteins for Degradation in Response to Meiotic Maturation.
MBK-2 functions as a temporal regulator of protein stability, and that coordinate activation of maternal protein degradation is one of the mechanisms that drives the transition from symmetric egg to patterned embryo.
Title: Coordinate activation of maternal protein degradation during the egg-to-embryo transition in C. elegans.
MBK-2 phosphorylates OMA-1 in vitro, primarily at threonine239; phosphorylation at T239 is required both for OMA-1 function in the 1-cell embryo and its degradation after the first mitosis
Title: DYRK2 and GSK-3 phosphorylate and promote the timely degradation of OMA-1, a key regulator of the oocyte-to-embryo transition in C. elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F49E11.1 (e-PCR)
- UniSTS:321491

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein serine/threonine/tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein tyrosine kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in P granule disassembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in P granule disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-tyrosine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2;dual-specificity kinase

NP_001023207.1

Confirmed by transcript evidence

NP_001023208.1

Confirmed by transcript evidence

NP_001040950.1

Confirmed by transcript evidence

NP_001040951.1

Confirmed by transcript evidence

NP_001255695.1

Confirmed by transcript evidence

NP_001293914.1

Confirmed by transcript evidence

NP_001293915.1

Confirmed by transcript evidence

NP_001293916.1

Confirmed by transcript evidence

NP_001293917.1

Confirmed by transcript evidence

NP_001366773.1

Confirmed by transcript evidence

NP_001368118.1

Confirmed by transcript evidence

NP_001368149.1

Confirmed by transcript evidence

NP_001368473.1

Partially confirmed by transcript evidence

NP_001368474.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

13009158..13035961

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001028037.4 → NP_001023208.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023208.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XTF3

Conserved Domains (2) summary

smart00220
Location:461 → 774

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14224
Location:395 → 774

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001028036.5 → NP_001023207.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023207.1

Status: REVIEWED

UniProtKB/Swiss-Prot

H9G2V8, H9G2V9, J7SF89, Q20604, Q27GP3, Q2EEP1, Q9TVF4, Q9XTF3

Conserved Domains (2) summary

smart00220
Location:461 → 774

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14224
Location:395 → 774

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001306987.4 → NP_001293916.1 dual-specificity kinase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293916.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A061AKR6

Conserved Domains (1) summary

cd14224
Location:378 → 757

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001380497.1 → NP_001368118.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd14224
Location:378 → 757

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001306988.3 → NP_001293917.1 dual-specificity kinase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293917.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A061ACN7

Conserved Domains (1) summary

cd14224
Location:113 → 492

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001380498.1 → NP_001368149.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd14224
Location:113 → 492

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001047486.4 → NP_001040951.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001040951.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XTF3

Conserved Domains (2) summary

smart00220
Location:160 → 473

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14224
Location:94 → 473

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001268766.4 → NP_001255695.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255695.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XTF3

Conserved Domains (2) summary

smart00220
Location:164 → 477

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14224
Location:98 → 477

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001380500.1 → NP_001366773.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd14224
Location:101 → 480

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001306985.3 → NP_001293914.1 dual-specificity kinase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293914.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A061ACK4

Conserved Domains (1) summary

cd14224
Location:101 → 480

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001380499.1 → NP_001368473.1 dual-specificity kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A061ACN3

Conserved Domains (1) summary

cd14224
Location:101 → 480

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001306986.3 → NP_001293915.1 dual-specificity kinase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001293915.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A061AJ20

Conserved Domains (1) summary

cd14224
Location:68 → 447

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001380501.1 → NP_001368474.1 dual-specificity kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A061ADU8

Conserved Domains (1) summary

cd14224
Location:68 → 447

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3
NM_001047485.5 → NP_001040950.1 Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001040950.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9XTF3

Conserved Domains (2) summary

smart00220
Location:134 → 447

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14224
Location:68 → 447

PKc_DYRK2_3; Catalytic domain of the protein kinases, Dual-specificity tYrosine-phosphorylated and -Regulated Kinases 2 and 3