Klf4 Kruppel-like transcription factor 4 (gut) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Klf4provided by MGI
Official Full Name: Kruppel-like transcription factor 4 (gut)provided by MGI
Primary source: MGI:MGI:1342287
See related: Ensembl:ENSMUSG00000003032 AllianceGenome:MGI:1342287
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EZF; Zie; Gklf
Summary: Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity; and nucleic acid binding activity. Involved in several processes, including establishment of skin barrier; fat cell differentiation; and positive regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; epithelial cell differentiation; and regulation of DNA-templated transcription. Located in cytoplasm; euchromatin; and nucleus. Part of transcription regulator complex. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; limb; and skin. Human ortholog(s) of this gene implicated in hepatocellular carcinoma. Orthologous to human KLF4 (KLF transcription factor 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in colon adult (RPKM 221.0), stomach adult (RPKM 155.0) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 B3; 4 29.76 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (55527137..55536984, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (55527137..55532475, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

KLF4 regulates trophoblast function and associates with unexplained recurrent spontaneous abortion.
Title: KLF4 regulates trophoblast function and associates with unexplained recurrent spontaneous abortion.
Interplay of RUNX2 and KLF4 in initial commitment of odontoblast differentiation.
Title: Interplay of RUNX2 and KLF4 in initial commitment of odontoblast differentiation.
Kruppel-like factor 4 in transcriptional control of the three unique isoforms of Agouti-related peptide in mice.
Title: Krüppel-like factor 4 in transcriptional control of the three unique isoforms of Agouti-related peptide in mice.
N6-methyladenosine-driven miR-143/145-KLF4 circuit orchestrates the phenotypic switch of pulmonary artery smooth muscle cells.
Title: N6-methyladenosine-driven miR-143/145-KLF4 circuit orchestrates the phenotypic switch of pulmonary artery smooth muscle cells.
KLF4 inhibited the senescence-associated secretory phenotype in ox-LDL-treated endothelial cells via PDGFRA/NAMPT/mitochondrial ROS.
Title: KLF4 inhibited the senescence-associated secretory phenotype in ox-LDL-treated endothelial cells via PDGFRA/NAMPT/mitochondrial ROS.
HEG1 Protects Against Atherosclerosis by Regulating Stable Flow-Induced KLF2/4 Expression in Endothelial Cells.
Title: HEG1 Protects Against Atherosclerosis by Regulating Stable Flow-Induced KLF2/4 Expression in Endothelial Cells.
Endothelium-specific SIRT7 targeting ameliorates pulmonary hypertension through Krupple-like factor 4 deacetylation.
Title: Endothelium-specific SIRT7 targeting ameliorates pulmonary hypertension through Krüpple-like factor 4 deacetylation.
KLF4 down-regulation underlies placental angiogenesis impairment induced by maternal glucose intolerance in late pregnancy.
Title: KLF4 down-regulation underlies placental angiogenesis impairment induced by maternal glucose intolerance in late pregnancy.
Effect of overexpression of KLF4 on the growth and development of hair follicles in mice.
Title: Effect of overexpression of KLF4 on the growth and development of hair follicles in mice.
Ovarian Tumor Domain-Containing 7B Attenuates Pathological Cardiac Hypertrophy by Inhibiting Ubiquitination and Degradation of Kruppel-Like Factor 4.
Title: Ovarian Tumor Domain-Containing 7B Attenuates Pathological Cardiac Hypertrophy by Inhibiting Ubiquitination and Degradation of Krüppel-Like Factor 4.

Submit: New GeneRIF Correction See all GeneRIFs (303)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (10) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC304100P5 (e-PCR)
- UniSTS:272530

Klf4 (e-PCR), detects polymorphism
- UniSTS:256949

Klf4 (e-PCR), detects polymorphism
- UniSTS:516861

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables beta-catenin binding	IPI Inferred from Physical Interaction more info	PubMed
enables chromatin DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	IEA Inferred from Electronic Annotation more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables lncRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol 3-kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info
enables promoter-specific chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription coregulator binding	IEA Inferred from Electronic Annotation more info
enables transcription coregulator binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cycloheximide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to growth factor stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hydrogen peroxide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to laminar fluid shear stress	IEA Inferred from Electronic Annotation more info
involved_in cellular response to laminar fluid shear stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to peptide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to retinoic acid	IEA Inferred from Electronic Annotation more info
involved_in defense response to tumor cell	IEA Inferred from Electronic Annotation more info
involved_in defense response to tumor cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within epidermal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epidermis morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of skin barrier	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glandular epithelial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in negative regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of G1/S transition of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of G1/S transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell migration involved in sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell migration involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell population proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of chemokine (C-X-C motif) ligand 2 production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of chemokine (C-X-C motif) ligand 2 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway in absence of ligand	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of heterotypic cell-cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-8 production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of leukocyte adhesion to arterial endothelial cell	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of leukocyte adhesion to arterial endothelial cell	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of muscle hyperplasia	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of response to cytokine stimulus	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of response to cytokine stimulus	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of hemoglobin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of hemoglobin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of miRNA transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere maintenance	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within post-embryonic camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-embryonic hemopoiesis	IEA Inferred from Electronic Annotation more info
involved_in post-embryonic hemopoiesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of axon regeneration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of axon regeneration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of blastocyst development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to retinoic acid	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within somatic stem cell population maintenance	IDA Inferred from Direct Assay more info	PubMed
involved_in stem cell population maintenance	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
part_of transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: Krueppel-like factor 4

Names: Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF); Kruppel-like factor 4 (gut); epithelial zinc finger protein EZF; gut-enriched krueppel-like factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010637.3 → NP_034767.2 Krueppel-like factor 4

See identical proteins and their annotated locations for NP_034767.2

Status: VALIDATED

Source sequence(s)

AA389216, AK141244, AL732494, BQ034021, CF163360

Consensus CDS

CCDS18195.2

UniProtKB/Swiss-Prot

P70421, Q3U2D6, Q3URS6, Q60793, Q78K30, Q9R255

Related

ENSMUSP00000103245.3, ENSMUST00000107619.3

Conserved Domains (4) summary

COG5048
Location:395 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:402 → 424

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:400 → 424

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:446 → 471

zf-H2C2_2; Zinc-finger double domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

55527137..55536984 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036163748.1 → XP_036019641.1 Krueppel-like factor 4 isoform X1

Conserved Domains (4) summary

COG5048
Location:345 → 428

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:352 → 374

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:396 → 421

zf-H2C2_2; Zinc-finger double domain

cl41729
Location:24 → 350

KLF1_2_4_N; N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins