Clk2 CDC-like kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Clk2provided by MGI
Official Full Name: CDC-like kinase 2provided by MGI
Primary source: MGI:MGI:1098669
See related: Ensembl:ENSMUSG00000068917 AllianceGenome:MGI:1098669
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables protein serine/threonine kinase activity and protein tyrosine kinase activity. Involved in negative regulation of gluconeogenesis and protein autophosphorylation. Acts upstream of or within peptidyl-tyrosine phosphorylation and response to retinoic acid. Located in nucleus. Is expressed in several structures, including brain; genitourinary system; liver; retina; and thyroid gland. Orthologous to human CLK2 (CDC like kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in limb E14.5 (RPKM 37.3), CNS E11.5 (RPKM 31.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F1; 3 39.01 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (89072102..89084394)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (89164795..89177087)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CLK2 mediates IkappaBalpha-independent early termination of NF-kappaB activation by inducing cytoplasmic redistribution and degradation.
Title: CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation.
CLK2 in GABAergic neurons is critical in regulating energy balance and anxiety-like behavior in a gender-specific fashion.
Title: CLK2 in GABAergic neurons is critical in regulating energy balance and anxiety-like behavior in a gender-specific fashion.
High CLK2 expression is associated with obesity.
Title: Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation.
CLK2 is a regulatory component of diet-induced thermogenesis in brown adipose tissue.
Title: Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.
Liver-specific Clk2 knockout mice fed a high-fat diet exhibit increased fasting levels of blood ketone bodies, reduced respiratory exchange ratio, and increased gene expression of fatty acid oxidation and ketogenic pathways.
Title: Cdc2-like kinase 2 suppresses hepatic fatty acid oxidation and ketogenesis through disruption of the PGC-1α and MED1 complex.
LAMMER/Cdc2-like kinase, represented by Doa and its mammalian homolog CLK2, is a critical and conserved component in the regulatory program of the mitosis-to-meiosis switch
Title: The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
Cdc2-like kinase 2 (Clk2) directly phosphorylates the SR domain on PGC-1alpha, resulting in repression of gluconeogenic gene expression and hepatic glucose output.
Title: Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU041688 (e-PCR)
- UniSTS:159135

Clk2 (e-PCR), detects polymorphism
- UniSTS:527289

Readthrough Gm45927

Readthrough gene: Gm45927, Included gene: Scamp3

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AXY142 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3Y41 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine/tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of RNA splicing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of RNA splicing	ISO Inferred from Sequence Orthology more info
involved_in regulation of RNA splicing	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to ionizing radiation	ISO Inferred from Sequence Orthology more info
involved_in response to ionizing radiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within response to retinoic acid	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dual specificity protein kinase CLK2

NP_001156904.1

EC 2.7.12.1

NP_031738.2

EC 2.7.12.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163432.2 → NP_001156904.1 dual specificity protein kinase CLK2 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AC161600, BY021174, BY247622, BY738871, CD740936, CN538691, DT924403

Consensus CDS

CCDS50958.1

UniProtKB/TrEMBL

E9Q5Y1, F8WHX0

Related

ENSMUSP00000088445.6, ENSMUST00000090927.12

Conserved Domains (2) summary

smart00220
Location:161 → 477

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14215
Location:148 → 477

PKc_CLK2; Catalytic domain of the Dual-specificity protein kinase, CDC-like kinase 2
NM_007712.4 → NP_031738.2 dual specificity protein kinase CLK2 isoform 1

See identical proteins and their annotated locations for NP_031738.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC161600, BY021174, BY247622, BY738871, CD740936, CN538691, DT924403

Consensus CDS

CCDS17491.1

UniProtKB/Swiss-Prot

E9Q721, O35491

UniProtKB/TrEMBL

E9Q5Y1

Related

ENSMUSP00000113390.3, ENSMUST00000121212.9

Conserved Domains (2) summary

smart00220
Location:162 → 478

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14215
Location:149 → 478

PKc_CLK2; Catalytic domain of the Dual-specificity protein kinase, CDC-like kinase 2