fem-1 Sex-determining protein fem-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: fem-1
Official Full Name: Sex-determining protein fem-1
Primary source: WormBase:WBGene00001411
Locus tag: CELE_F35D6.1
See related: AllianceGenome:WB:WBGene00001411
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables protein phosphatase binding activity. Involved in several processes, including male germ-line sex determination; male somatic sex determination; and masculinization of hermaphroditic germ-line. Part of Cul2-RING ubiquitin ligase complex. Is expressed in several structures, including germ line and in male. Orthologous to several human genes including FEM1A (fem-1 homolog A) and FEM1C (fem-1 homolog C). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See fem-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 11

Sequence:: Chromosome: IV; NC_003282.8 (5533140..5537962, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicate that the N-terminal domain (NTD) works in mediating the sex-determining role of FEM-1/2/3 complex.
Title: Structural insight into Caenorhabditis elegans sex-determining protein FEM-2.
maternal fem-1 transcript is required to license expression of wild-type fem-1 allele in zygotic germline; defect in zygotic fem-1 expression is heritable, suggesting gene is subject to epigenetic silencing that is prevented by maternal fem-1 transcripts
Title: Epigenetic licensing of germline gene expression by maternal RNA in C. elegans.
Fem-1 protein is a substrate-specific adaptor for a CUL-2-based ubiquitin ligase.
Title: PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditiselegans.
Results suggest that SEL-10-mediated proteolysis of FEM-1 and FEM-3 is required for normal hermaphrodite development.
Title: The Caenorhabditis elegans F-box protein SEL-10 promotes female development and may target FEM-1 and FEM-3 for degradation by the proteasome.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T12E12.4 (e-PCR)
- UniSTS:310820

F35D6.1 (e-PCR)
- UniSTS:310125

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-like ligase-substrate adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in male germ-line sex determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male somatic sex determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in masculinization of hermaphroditic germ-line	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of germ cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sex differentiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Cul2-RING ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Sex-determining protein fem-1

NP_001379561.1

Confirmed by transcript evidence

NP_500824.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

5533140..5537962 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001392312.1 → NP_001379561.1 Sex-determining protein fem-1 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (3) summary

sd00045
Location:47 → 73

ANK; ANK repeat [structural motif]

pfam00023
Location:47 → 77

Ank; Ankyrin repeat

cl39094
Location:9 → 77

Ank_2; Ankyrin repeats (3 copies)
NM_068423.7 → NP_500824.1 Sex-determining protein fem-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_500824.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P17221, Q95ZU3

Conserved Domains (5) summary

cd00204
Location:83 → 209

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam00023
Location:47 → 77

Ank; Ankyrin repeat

pfam12796
Location:93 → 185

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:189 → 240

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:125 → 153

ANK; ANK repeat [structural motif]