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    HSI2 B3 domain-containing transcription repressor VAL1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 817597, updated on 18-Sep-2024

    Summary

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    Official Symbol
    HSI2
    Official Full Name
    B3 domain-containing transcription repressor VAL1
    Primary source
    TAIR:AT2G30470
    Locus tag
    AT2G30470
    See related
    Araport:AT2G30470
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    high-level expression of sugar-inducible gene 2; T6B20.17; T6B20_17; VAL1; VP1/ABI3-LIKE 1
    Summary
    HSI2 is a member of a novel family of B3 domain proteins with a sequence similar to the ERF-associated amphiphilic repression (EAR) motif. It functions as an active repressor of the Spo minimal promoter (derived from a gene for sweet potato sporamin A1) through the EAR motif. It contains a plant-specific B3 DNA-binding domain. The Arabidopsis genome contains 42 genes with B3 domains which could be classified into three families that are represented by ABI3, ARF1 and RAV1. HSI2 belongs to the ABI3 family. It is expressed at similar levels in all organs. Treatment with 6% sucrose showed a slight increase in transcript levels after 24 h. No changes were observed after treatment with 50##M ABA. It is localized in the nucleus via a nuclear localization sequence located in the fourth conserved region of the C-terminal B3 domain.
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    Genomic context

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    See HSI2 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    12
    Sequence:
    Chromosome: 2; NC_003071.7 (12980428..12985277, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene tRNA-Gly Neighboring gene Nucleotide/sugar transporter family protein Neighboring gene uncharacterized protein Neighboring gene cinnamate-4-hydroxylase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis. Baile F, et al. Plant Cell, 2021 Aug 31. PMID 34003929, Free PMC Article
    2. Analysis of a sugar response mutant of Arabidopsis identified a novel B3 domain protein that functions as an active transcriptional repressor. Tsukagoshi H, et al. Plant Physiol, 2005 Jun. PMID 15894743, Free PMC Article
    3. Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1. Sasnauskas G, et al. Nucleic Acids Res, 2018 May 4. PMID 29660015, Free PMC Article
    4. Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization. Qüesta JI, et al. Science, 2016 Jul 29. PMID 27471304
    5. HSI2/VAL1 PHD-like domain promotes H3K27 trimethylation to repress the expression of seed maturation genes and complex transgenes in Arabidopsis seedlings. Veerappan V, et al. BMC Plant Biol, 2014 Nov 1. PMID 25367506, Free PMC Article

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Brassinosteroids repress the seed maturation program during the seed-to-seedling transition.
      Title: Brassinosteroids repress the seed maturation program during the seed-to-seedling transition.
    2. EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis.
      Title: EAR domain-containing transcription factors trigger PRC2-mediated chromatin marking in Arabidopsis.
    3. VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms.
      Title: VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms.
    4. The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis.
      Title: The transcriptional repressors VAL1 and VAL2 recruit PRC2 for genome-wide Polycomb silencing in Arabidopsis.
    5. The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T.
      Title: The B3-Domain Transcription Factor VAL1 Regulates the Floral Transition by Repressing FLOWERING LOCUS T.
    6. Structural basis for the Sph/RY sequence recognition by VAL1/2 transcriptional repressor.
      Title: Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1.
    7. VAL1 crystal structure provides insight into the important implication of VAL1-B3 domain in flowering process.
      Title: Structural insight into the role of VAL1 B3 domain for targeting to FLC locus in Arabidopsis thaliana.
    8. The results suggest that HSI2 recruits MED13 and HDA6 to suppress directly a subset of seed maturation genes post-germination.
      Title: HSI2 Repressor Recruits MED13 and HDA6 to Down-Regulate Seed Maturation Gene Expression Directly During Arabidopsis Early Seedling Growth.
    9. LEC1, LEC2 , and FUSCA3 transcripts are candidate targets of VAL1, acting through epigenetic and/or transcriptional repression.
      Title: Potential targets of VIVIPAROUS1/ABI3-LIKE1 (VAL1) repression in developing Arabidopsis thaliana embryos.
    10. VAL1 localizes to the nucleation region in vivo, promoting histone deacetylation and FLOWERING LOCUS C transcriptional silencing, and interacts with components of the conserved apoptosis- and splicing-associated protein complex.
      Title: Arabidopsis transcriptional repressor VAL1 triggers Polycomb silencing at FLC during vernalization.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_179815.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables DNA-binding transcription factor activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of seed germination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of seed maturation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within response to abscisic acid TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to sucrose TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in mitochondrion HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    B3 domain-containing transcription repressor VAL1
    NP_850146.1
    • high-level expression of sugar-inducible gene 2 (HSI2); CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340), Zinc finger, CW-type (InterPro:IPR011124); BEST Arabidopsis thaliana protein match is: HSI2-like 1 (TAIR:AT4G32010.1); Has 1263 Blast hits to 1242 proteins in 97 species: Archae - 0; Bacteria - 5; Metazoa - 31; Fungi - 0; Plants - 1168; Viruses - 0; Other Eukaryotes - 59 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      12980428..12985277 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_179815.3 → NP_850146.1  B3 domain-containing transcription repressor VAL1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850146.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O04346, Q8W4L5
      UniProtKB/TrEMBL
      A0A178VNU8, A0A5S9X2U9
      Conserved Domains (2) summary
      cd10017
      Location:293 → 390
      B3_DNA; Plant-specific B3-DNA binding domain
      pfam07496
      Location:544 → 585
      zf-CW; CW-type Zinc Finger
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8W4L5.1

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