GRP7 cold, circadian rhythm, and rna binding 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: GRP7
Official Full Name: cold, circadian rhythm, and rna binding 2
Primary source: TAIR:AT2G21660
Locus tag: AT2G21660
See related: Araport:AT2G21660
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ''cold; and rna binding 2; and rna binding 2''; ATGPR7; ATGRP7; CCR2; circadian rhythm; cold; F2G1.4; GLYCINE RICH PROTEIN 7; GLYCINE-RICH RNA-BINDING PROTEIN 7; GR-RBP7
Summary: Encodes a small glycine-rich RNA binding protein that is part of a negative-feedback loop through which AtGRP7 regulates the circadian oscillations of its own transcript. Gene expression is induced by cold. GRP7 appears to promote stomatal opening and reduce tolerance under salt and dehydration stress conditions, but, promotes stomatal closing and thereby increases stress tolerance under conditions of cold tolerance. Loss of function mutations have increased susceptibility to pathogens suggesting a role in mediating innate immune response. Mutants are also late flowering in a non-photoperiodic manner and are responsive to vernalization suggesting an interaction with the autonomous flowering pathway. There is a reduction of mRNA export from the nucleus in grp7 mutants. GRP7:GFP fusion proteins can be found in the cytosol and nucleus. A substrate of the type III effector HopU1 (mono-ADP-ribosyltransferase).
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Genomic context

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See GRP7 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 2

Sequence:: Chromosome: 2; NC_003071.7 (9265084..9266557, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The glycine-rich domain of GRP7 plays a crucial role in binding long RNAs and facilitating phase separation.
Title: The glycine-rich domain of GRP7 plays a crucial role in binding long RNAs and facilitating phase separation.
Arabidopsis thaliana GLYCINE RICH RNA-BINDING PROTEIN 7 interaction with its iCLIP target LHCB1.1 correlates with changes in RNA stability and circadian oscillation.
Title: Arabidopsis thaliana GLYCINE RICH RNA-BINDING PROTEIN 7 interaction with its iCLIP target LHCB1.1 correlates with changes in RNA stability and circadian oscillation.
Our results suggest that the RNA-binding proteins (RBPs) AtGRP7 and AtGRP8 influence MADS-Box transcription factors in at least two different pathways of flowering time control.
Title: Regulation of Flowering Time by the RNA-Binding Proteins AtGRP7 and AtGRP8.
Identification of a set of high-confidence targets of AtGRP7, some of which are regulated by AtGRP7 at the level of steady-state abundance or splicing.
Title: Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7.
AtJAC1 coordinates with GRP7 in shaping plant development through the regulation of RNA processing in Arabidopsis.
Title: JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis.
Truncation of the glycine-rich domain reduces the binding affinity of AtGRP7, showing for the first time that the glycine-rich stretch of a plant hnRNP-like protein contributes to binding.
Title: Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy.
GRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs.
Title: Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.
AtGRP7 has both salicylic acid (SA)-dependent and SA-independent effects on plant immunity.
Title: Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity.
AtGRP7-overexpressing plants flower with fewer leaves than wild-type plants, suggesting that the floral promotive effect of AtGRP7 bypasses the effect of a reduced gibberellin level.
Title: A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis.
Data indicate that AtGRP2 and AtGRP7 affected the expression of drought stress-responsive genes in transgenic rice.
Title: Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Oryza sativa) under drought stress conditions.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_127738.5

Supported by ESTs:

NM_179686.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within RNA secondary structure unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within circadian rhythm	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within circadian rhythm	TAS Traceable Author Statement more info	PubMed
involved_in extracellular transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within innate immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of defense response to virus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to cold	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to cold	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to salt stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to water deprivation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to zinc ion	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within vegetative to reproductive phase transition of meristem	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in peroxisome	HDA more info	PubMed
located_in plasmodesma	HDA more info	PubMed
located_in vacuole	HDA more info	PubMed

General protein information

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Preferred Names: cold, circadian rhythm, and rna binding 2

NP_179760.1

''cold, circadian rhythm, and rna binding 2'' (CCR2); FUNCTIONS IN: double-stranded DNA binding, RNA binding, single-stranded DNA binding; INVOLVED IN: in 12 processes; LOCATED IN: nucleus, chloroplast, peroxisome, cytoplasm; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: RNA recognition motif, glycine rich protein (InterPro:IPR015465), RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: cold, circadian rhythm, and RNA binding 1 (TAIR:AT4G39260.3); Has 548 Blast hits to 547 proteins in 63 species: Archae - 0; Bacteria - 0; Metazoa - 92; Fungi - 0; Plants - 453; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink).

NP_850017.1

GLYCINE RICH PROTEIN 7 (ATGRP7); FUNCTIONS IN: double-stranded DNA binding, RNA binding, single-stranded DNA binding; INVOLVED IN: in 12 processes; LOCATED IN: nucleus, chloroplast, peroxisome, cytoplasm; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: RNA recognition motif, glycine rich protein (InterPro:IPR015465), RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: cold, circadian rhythm, and RNA binding 1 (TAIR:AT4G39260.3); Has 381 Blast hits to 381 proteins in 95 species: Archae - 0; Bacteria - 0; Metazoa - 191; Fungi - 60; Plants - 124; Viruses - 0; Other Eukaryotes - 6 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

9265084..9266557 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_127738.5 → NP_179760.1 cold, circadian rhythm, and rna binding 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_179760.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0A6B9JEL0, C0Z304, Q03250, Q8LEV4, Q94B62

UniProtKB/TrEMBL

A0A5S9WZY2, A0A654EUX5

Conserved Domains (1) summary

cd21608
Location:9 → 84

RRM2_NsCP33_like; RNA recognition motif 2 (RRM2) found in Nicotiana sylvestris chloroplastic 33 kDa ribonucleoprotein (NsCP33) and similar proteins
NM_179686.2 → NP_850017.1 cold, circadian rhythm, and rna binding 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850017.1

Status: REVIEWED

UniProtKB/TrEMBL

F4IHK9

Conserved Domains (1) summary

cl17169
Location:11 → 85

RRM_SF; RNA recognition motif (RRM) superfamily