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    RAD18 RAD18 E3 ubiquitin protein ligase [ Homo sapiens (human) ]

    Gene ID: 56852, updated on 10-Dec-2024

    Summary

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    Official Symbol
    RAD18provided by HGNC
    Official Full Name
    RAD18 E3 ubiquitin protein ligaseprovided by HGNC
    Primary source
    HGNC:HGNC:18278
    See related
    Ensembl:ENSG00000070950 MIM:605256; AllianceGenome:HGNC:18278
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    RNF73
    Summary
    The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in duodenum (RPKM 3.3), lymph node (RPKM 2.7) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See RAD18 in Genome Data Viewer
    Location:
    3p25.3
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (8877075..8963472, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (8868283..8960543, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (8918759..9005156, complement)

    Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107986061 Neighboring gene uncharacterized LOC107984112 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr3:8911034-8912233 Neighboring gene small nucleolar RNA SNORA43 Neighboring gene Sharpr-MPRA regulatory region 4351 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19395 Neighboring gene Sharpr-MPRA regulatory region 3594 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9029229-9029729 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9028728-9029228 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9038453-9038952 Neighboring gene SLIT-ROBO Rho GTPase activating protein 3 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9119367-9119867 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:9146925-9147681 Neighboring gene uncharacterized LOC124909343 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19396 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:9221274-9221774 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14030 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14031 Neighboring gene SRGAP3 antisense RNA 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. circRAD18 sponges miR-208a/3164 to promote triple-negative breast cancer progression through regulating IGF1 and FGF2 expression. Zou Y, et al. Carcinogenesis, 2019 Dec 31. PMID 31001629
    2. Dynamic localization of human RAD18 during the cell cycle and a functional connection with DNA double-strand break repair. Inagaki A, et al. DNA Repair (Amst), 2009 Feb 1. PMID 19013543
    3. Elevated expression of Rad18 regulates melanoma cell proliferation. Wong RP, et al. Pigment Cell Melanoma Res, 2012 Mar. PMID 22145991
    4. RAD18 promotes colorectal cancer metastasis by activating the epithelial‑mesenchymal transition pathway. Li P, et al. Oncol Rep, 2020 Jul. PMID 32319669, Free PMC Article
    5. High RAD18 Expression is Associated with Disease Progression and Poor Prognosis in Patients with Gastric Cancer. Baatar S, et al. Ann Surg Oncol, 2020 Oct. PMID 32356270

    See all (190) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Transcription Factor E2F7 Hampers the Killing Effect of NK Cells against Colorectal Cancer Cells via Activating RAD18 Transcription.
      Title: Transcription Factor E2F7 Hampers the Killing Effect of NK Cells against Colorectal Cancer Cells via Activating RAD18 Transcription.
    2. Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
      Title: Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
    3. Analysis of the Mechanism of RAD18 in Glioma.
      Title: Analysis of the Mechanism of RAD18 in Glioma.
    4. RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
      Title: RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
    5. RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
      Title: RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
    6. Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
      Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
    7. UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
      Title: UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
    8. REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
      Title: REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
    9. RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
      Title: RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
    10. Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
      Title: Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
    Submit: New GeneRIF Correction See all GeneRIFs (83)

    Phenotypes

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    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    integrase gag-pol HIV-1 Integrase interacts with (co-immunoprecipitates) and co-localizes with hRad18 suggesting a role for hRad18 in the DNA repair events associated with the HIV-1 integration process PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables Y-form DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables damaged DNA binding NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables polyubiquitin modification-dependent protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-containing complex binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA damage response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of chromosome segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in postreplication repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein autoubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein monoubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of Rad6-Rad18 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nuclear body IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nuclear inclusion body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in replication fork IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in site of double-strand break IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase RAD18
    Names
    RAD18 homolog
    RAD18, S. cerevisiae, homolog
    RING finger protein 73
    RING-type E3 ubiquitin transferase RAD18
    hHR18
    hRAD18
    postreplication repair protein RAD18
    postreplication repair protein hRAD18p
    NP_064550.3
    XP_016862362.1
    XP_054203240.1
    XP_054203241.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_020165.4 → NP_064550.3  E3 ubiquitin-protein ligase RAD18

      See identical proteins and their annotated locations for NP_064550.3

      Status: REVIEWED

      Source sequence(s)
      AC008151, AC034186, AK023075, DW433240
      Consensus CDS
      CCDS2571.1
      UniProtKB/Swiss-Prot
      Q58F55, Q9NRT6, Q9NS91
      UniProtKB/TrEMBL
      Q53H10
      Related
      ENSP00000264926.2, ENST00000264926.7
      Conserved Domains (4) summary
      smart00734
      Location:201 → 224
      ZnF_Rad18; Rad18-like CCHC zinc finger
      cd00162
      Location:25 → 63
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      TIGR00599
      Location:13 → 363
      rad18; DNA repair protein rad18
      pfam02037
      Location:248 → 282
      SAP; SAP domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

      Range
      8877075..8963472 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017006873.2 → XP_016862362.1  E3 ubiquitin-protein ligase RAD18 isoform X1

      UniProtKB/TrEMBL
      Q53H10

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060927.1 Alternate T2T-CHM13v2.0

      Range
      8868283..8960543 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054347265.1 → XP_054203240.1  E3 ubiquitin-protein ligase RAD18 isoform X2

      UniProtKB/TrEMBL
      Q53H10

    2. XM_054347266.1 → XP_054203241.1  E3 ubiquitin-protein ligase RAD18 isoform X1

      UniProtKB/TrEMBL
      Q53H10
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9NS91.2 GenPept UniProtKB/Swiss-Prot:Q9NS91

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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